Abstract
Crystalline ovalbumin, when heated under controlled conditions, promoted growth of Streptococcus hemolyticus in a synthetic medium. A similar activation of albumin also was produced by ultraviolet irradiation or by shaking under nitrogen. Untreated albumin failed to permit growth under the same conditions. The activity of treated albumin was destroyed by aeration or by reaction with iodoacetate. The data suggest that the activity of ovalbumin for the streptococcus depends on the presence of "exposed" SH groups. However, other sulfhydryl-containing compounds (thioglycolate, glutathione, cysteine) could not replace ovalbumin. A number of crude and crystalline proteins, and di- and tripeptides were inactive. Strepogenin and crystalline bovine serum albumin produced a weak growth response on extended incubation. Approximately 50 per cent of the original protein in the culture disappeared during growth. Ovalbumin labelled with C14 was added to the synthetic medium. After growth of the culture the isotope was found associated with the cells and in the culture fluid in both dialyzable and non-dialyzable forms. Under the conditions described, luxuriant growth of 15 strains (10 serological types) of Group A streptococci was obtained in 10 hours from small inocula. The growth was capable of repeated subcultivation.
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Selected References
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- ANFINSEN C. B., STEINBERG D. Studies on the biosynthesis of ovalbumin. J Biol Chem. 1951 Apr;189(2):739–744. [PubMed] [Google Scholar]
- Bernheimer A. W., Pappenheimer A. M. Factors Necessary for Massive Growth of Group A Hemolytic Streptococcus. J Bacteriol. 1942 Apr;43(4):481–494. doi: 10.1128/jb.43.4.481-494.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HESS E. L., SLADE H. D. An electrophoretic examination of cell-free extracts from various serological types of group A hemolytic streptococci. Biochim Biophys Acta. 1955 Mar;16(3):346–353. doi: 10.1016/0006-3002(55)90237-7. [DOI] [PubMed] [Google Scholar]
- Neuberger A. Carbohydrates in protein: The carbohydrate component of crystalline egg albumin. Biochem J. 1938 Sep;32(9):1435–1451. doi: 10.1042/bj0321435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SLADE H. D., KNOX G. A. Nutrition and the role of reducing agents in the formation of streptolysin O by a group A hemolytic streptococcus. J Bacteriol. 1950 Sep;60(3):301–310. doi: 10.1128/jb.60.3.301-310.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SLADE H. D., KNOX G. A., SLAMP W. C. The amino acid nutrition of group A hemolytic Streptococci, with reference to the effect of glutathione on the cystine requirement. J Bacteriol. 1951 Nov;62(5):669–675. doi: 10.1128/jb.62.5.669-675.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STEINBERG D., ANFINSEN C. B. Evidence for intermediates in ovalbumin synthesis. J Biol Chem. 1952 Nov;199(1):25–42. [PubMed] [Google Scholar]
- SUTHERLAND E. W., CORI C. F. Purification of the hyperglycemic-glycogenolytic factor from insulin and from gastric mucosa. J Biol Chem. 1949 Sep;180(2):825–837. [PubMed] [Google Scholar]
- Todrick A., Walker E. Sulphydryl groups in proteins. Biochem J. 1937 Feb;31(2):292–296. doi: 10.1042/bj0310292. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woolley D. W. A NEW GROWTH FACTOR REQUIRED BY CERTAIN HEMOLYTIC STREPTOCOCCI. J Exp Med. 1941 Mar 31;73(4):487–492. doi: 10.1084/jem.73.4.487. [DOI] [PMC free article] [PubMed] [Google Scholar]