Abstract
A culture of HeLa cells has been subjected to prolonged observation with the finding that periodically Type III poliomyelitis virus could be isolated from it. A requirement of the culture for survival was the presence in it of serum of certain individuals who had had previous experience with poliomyelitis virus. In the presence of serum containing no antibodies to poliomyelitis virus, the culture demonstrated spontaneous cytopathology. From certain series of passages virus could be isolated while attempts were unsuccessful from others also showing cellular disintegration. The conclusion is reached that the virus does not persist in the culture always in a state exhibiting the infectious property, rather what persists is the potentiality of the culture to give rise to fully active virus. The immune serum could inhibit the cytopathogenic effect of the virus without eliminating the infection.
Full Text
The Full Text of this article is available as a PDF (606.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ACKERMANN W. W., FRANCIS T., Jr Characteristics of viral development in isolated animal tissues. Adv Virus Res. 1954;2:81–108. doi: 10.1016/s0065-3527(08)60530-8. [DOI] [PubMed] [Google Scholar]
- ACKERMANN W. W., RABSON A., KURTZ H. Growth characteristics of poliomyelitis virus in HeLa cell cultures; lack of parallelism in cellular injury and virus increase. J Exp Med. 1954 Nov 1;100(5):437–450. doi: 10.1084/jem.100.5.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coggeshall L. T., Eaton M. D. THE COMPLEMENT FIXATION REACTION IN MONKEY MALARIA. J Exp Med. 1938 May 31;67(6):871–882. doi: 10.1084/jem.67.6.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Francis T., Jr MECHANISMS OF INFECTION AND IMMUNITY IN VIRUS DISEASES OF MAN. Bacteriol Rev. 1947 Sep;11(3):147–156. doi: 10.1128/br.11.3.147-156.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HENLE G., GIRARDI A., HENLE W. A non-transmissible cytopathogenic effect of influenza virus in tissue culture accompanied by formation of non-infectious hemagglutinins. J Exp Med. 1955 Jan 1;101(1):25–41. doi: 10.1084/jem.101.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ISAACS A., FULTON F. Growth of influenza virus in the chick chorion. Nature. 1953 Jan 10;171(4341):90–90. doi: 10.1038/171090a0. [DOI] [PubMed] [Google Scholar]
- ISAACS A., FULTON F. Interference in the chick chorion. J Gen Microbiol. 1953 Aug;9(1):132–139. doi: 10.1099/00221287-9-1-132. [DOI] [PubMed] [Google Scholar]
- SYVERTON J. T., SCHERER W. F., ELWOOD P. M. Studies on the propagation in vitro of poliomyelitis viruses. V. The application of strain HeLa human epithelial cells for isolation and typing. J Lab Clin Med. 1954 Feb;43(2):286–302. [PubMed] [Google Scholar]