Abstract
A technique has been described whereby tuberculin PPD is coupled, via tetrazotized benzidne, onto the surfaces of formalinized red blood cells. A stable antigen preparation is thereby obtained, which may be stored at –40°C. The purpose of the present method is to facilitate the study of antibody to tuberculoprotein.
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Selected References
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- BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baldwin E. R., Trudeau E. L. Hypersusceptibility or Anaphylaxis. J Med Res. 1910 Apr;22(2):189–256. [PMC free article] [PubMed] [Google Scholar]
- COLE L. R., FAVOUR C. B. Correlations between plasma protein fractions, antibody titers, and the passive transfer of delayed and immediate cutaneous reactivity to tuberculin PPD and tuberculopolysaccharides. J Exp Med. 1955 Apr 1;101(4):391–420. doi: 10.1084/jem.101.4.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COOMBS R. R. A., HOWARD A. N., WILD F. Titration of antisera to soluble proteins on the basis of an agglutination reaction: conjugation of egg albumin and chicken serum globulin to the incomplete Rh antibody and the subsequent use of Rh-positive cells, sensitized by such conjugated incomplete antibodies, to titrate antisera against egg albumin and chicken globulin. Br J Exp Pathol. 1952 Aug;33(4):390–397. [PMC free article] [PubMed] [Google Scholar]
- Enders J. F. ANAPHYLACTIC SHOCK WITH THE PARTIAL ANTIGEN OF THE TUBERCLE BACILLUS. J Exp Med. 1929 Nov 30;50(6):777–786. doi: 10.1084/jem.50.6.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRABAR P., BOYDEN S., TAQUET A., BORDUAS A. Mise en évidence de deux anticorps différents dans les sérums tuberculeux par hémagglutination passive. C R Hebd Seances Acad Sci. 1952 Feb 18;234(8):899–901. [PubMed] [Google Scholar]
- ISLIKER H. C. Purification of antibodies by means of antigens linked to ion exchange resins. Ann N Y Acad Sci. 1953 Nov 11;57(3):225–238. doi: 10.1111/j.1749-6632.1953.tb36397.x. [DOI] [PubMed] [Google Scholar]
- MIDDLEBROOK G. Antigens of tubercle bacillus involved in hemagglutination and hemolysis reactions. Bull N Y Acad Med. 1952 Jul;28(7):474–475. [PMC free article] [PubMed] [Google Scholar]
- MIDDLEBROOK G., DUBOS R. J. Specific serum agglutination of erythrocytes sensitized with extracts of tubercle bacilli. J Exp Med. 1948 Nov;88(5):521–528. doi: 10.1084/jem.88.5.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zinsser H., Parker J. T. FURTHER STUDIES ON BACTERIAL HYPERSUSCEPTIBILITY. II. J Exp Med. 1923 Jan 31;37(2):275–302. doi: 10.1084/jem.37.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]