Abstract
It has been shown by use of isolated, perfused rat kidneys that hypertensin II is a potent vasoconstrictor substance while hypertensin I is not. Hence it would appear that in intact animals the pressor activity of hypertensin I results from its rapid conversion to hypertensin II. An enzyme which effects this conversion has been procured from horse plasma in a semipurified form by means of ammonium sulfate fractionation and isoelectric precipitation. A method is described for estimating the activity of the enzyme. An example of the use of the preparation in converting purified hypertensin I to hypertensin II has been described.
Full Text
The Full Text of this article is available as a PDF (353.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- MOORE S., STEIN W. H. A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J Biol Chem. 1954 Dec;211(2):907–913. [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, KAHN J. R., MARSH W. H. A method of assaying small amounts of hypertensin. Lab Invest. 1953 Mar-Apr;2(2):109–114. [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, LEONARDS J. R., HEISLER C. R. Artificial kidney; construction and operation of an improved continuous dialyzer. Proc Soc Exp Biol Med. 1949 Dec;72(3):539–543. doi: 10.3181/00379727-72-17491. [DOI] [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, MARSH W. H., KAHN J. R., SHUMWAY N. P. Amino acid composition and electrophoretic properties of hypertensin I. J Exp Med. 1955 Oct 1;102(4):435–440. doi: 10.1084/jem.102.4.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, MARSH W. H., KAHN J. R., SHUMWAY N. P. The existence of two forms of hypertensin. J Exp Med. 1954 Mar;99(3):275–282. doi: 10.1084/jem.99.3.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, MARSH W. H., KAHN J. R., SHUMWAY N. P. The purification of hypertensin I. J Exp Med. 1954 Oct 1;100(4):363–370. doi: 10.1084/jem.100.4.363. [DOI] [PMC free article] [PubMed] [Google Scholar]