Abstract
The enzymatic conversion of hypertensin I to hypertensin II is described together with the subsequent purification of the product by means of counter-current distribution. Improved methods are also presented for the preparation of renin and its substrate, as well as in methods for the reaction of these materials and the purification of the resulting hypertensin I.
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Selected References
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- CRAIG L. C. Countercurrent distribution. Methods Med Res. 1952;5:3–24. [PubMed] [Google Scholar]
- Dexter L., Haynes F. W., Bridges W. C. THE RENAL HUMORAL PRESSOR MECHANISM IN MAN. I. PREPARATION AND ASSAY OF HUMAN RENIN, HUMAN HYPERTENSINOGEN, AND HYPERTENSIN. J Clin Invest. 1945 Jan;24(1):62–68. doi: 10.1172/JCI101579. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAHN J. R., SKEGGS L. T., Jr, SHUMWAY N. P., WISENBAUGH P. E. The assay of hypertensin from the arterial blood of normotensive and hypertensive human beings. J Exp Med. 1952 Jun;95(6):523–529. doi: 10.1084/jem.95.6.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Katz Y. J., Goldblatt H. STUDIES ON EXPERIMENTAL HYPERTENSION : XXI. THE PURIFICATION OF RENIN. J Exp Med. 1943 Jul 1;78(1):67–74. doi: 10.1084/jem.78.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, KAHN J. R., MARSH W. H. A method of assaying small amounts of hypertensin. Lab Invest. 1953 Mar-Apr;2(2):109–114. [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, KAHN J. R., SHUMWAY N. P. The isolation and assay of hypertensin from blood. J Exp Med. 1952 Mar;95(3):241–246. doi: 10.1084/jem.95.3.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, KAHN J. R., SHUMWAY N. P. The preparation and function of the hypertensin-converting enzyme. J Exp Med. 1956 Mar 1;103(3):295–299. doi: 10.1084/jem.103.3.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, MARSH W. H., KAHN J. R., SHUMWAY N. P. Amino acid composition and electrophoretic properties of hypertensin I. J Exp Med. 1955 Oct 1;102(4):435–440. doi: 10.1084/jem.102.4.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, MARSH W. H., KAHN J. R., SHUMWAY N. P. The existence of two forms of hypertensin. J Exp Med. 1954 Mar;99(3):275–282. doi: 10.1084/jem.99.3.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SKEGGS L. T., Jr, MARSH W. H., KAHN J. R., SHUMWAY N. P. The purification of hypertensin I. J Exp Med. 1954 Oct 1;100(4):363–370. doi: 10.1084/jem.100.4.363. [DOI] [PMC free article] [PubMed] [Google Scholar]