Abstract
Leucocytes were obtained from the blood of rabbits, incubated in vitro with soluble antigenic material derived from Shigella paradysenteriae, washed, and transferred to irradiated recipient animals. On the 4th day after cell transfer, agglutinins to Shigella appeared in the sera of the majority of such recipients. The distribution of maximum agglutinin titers was substantially lower in these animals than in recipients of lymph node cells similarly incubated, as reported in earlier studies. Recipients of iodoacetate-treated cells showed no agglutinins. Similar results were obtained with cells of peritoneal exudates obtained 9 days after the intraperitoneal injection of lanolin and light mineral oil. When cells of peritoneal exudates obtained 1 or 2 days after the injection of this irritant were incubated with the antigenic material and transferred to irradiated recipients, agglutinins did not appear in the sera of the latter. Similar results were obtained with cells from peritoneal exudates obtained a few days after the intraperitoneal injection of heavy mineral oil. The preponderant cell types in the suspensions from these two sources were the polymorphonuclear neutrophils and monocytes, respectively.
Full Text
The Full Text of this article is available as a PDF (674.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ERSLEV A. Antibody in lymphocytes from hyperimmunized rabbits. J Immunol. 1951 Oct;67(4):281–288. [PubMed] [Google Scholar]
- Ehrich W. E., Harris T. N., Mertens E. THE ABSENCE OF ANTIBODY IN THE MACROPHAGES DURING MAXIMUM ANTIBODY FORMATION. J Exp Med. 1946 Apr 30;83(5):373–381. [PMC free article] [PubMed] [Google Scholar]
- FARBER M. B., HARRIS S., HARRIS T. N., OGBURN C. A. Studies on the transfer of lymph node cells. VII. Transfer of cells incubated in vitro with filtrates of trypsin-treated suspensions of Shigella paradysenteriae. J Exp Med. 1956 Nov 1;104(5):645–661. doi: 10.1084/jem.104.5.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARRIS S., HARRIS T. N. Studies on the transfer of lymph node cells. V. Transfer of cells incubated in vitro with suspensions of Shigella paradysenteriae. J Immunol. 1955 Apr;74(4):318–328. [PubMed] [Google Scholar]
- HARRIS T. N., HARRIS S., FARBER M. B. Studies on the transfer of lymph node cells. II. Effects of experimental manipulation of the donor system. J Immunol. 1954 Feb;72(2):161–171. [PubMed] [Google Scholar]
- ROBERTS K. B. The failure of macrophages to produce antibodies. Br J Exp Pathol. 1955 Apr;36(2):199–204. [PMC free article] [PubMed] [Google Scholar]