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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1956 May 1;103(5):553–575. doi: 10.1084/jem.103.5.553

THE PROPERDIN SYSTEM AND IMMUNITY

V. THE BACTERICIDAL ACTIVITY OF THE PROPERDIN SYSTEM

Alastair C Wardlaw 1, Louis Pillemer 1
PMCID: PMC2136629  PMID: 13319578

Abstract

Methods for the preparation and standardization of reagents suitable for studies on the bactericidal action of the properdin system are described. The preparation and properties of serum free of properdin (RPb) are presented in detail because of the necessity for a suitable RPb in these studies. The properdin system is responsible for the bactericidal action of normal human serum against a variety of microorganisms. The present work shows that the removal of properdin from serum also removes bactericidal activity. Addition of properdin to properdin-deficient serum restores bactericidal activity. A quantitative relationship exists between the final properdin concentration and bactericidal activity against sensitive organisms. The possibilities of a bactericidal assay for properdin are discussed. It is demonstrated that, in addition to properdin, the four components of complement (present in RPb) are necessary for the destruction of properdinsensitive bacteria. If any component is missing, bactericidal activity is lost; when the component is replaced, bactericidal activity is restored. Magnesium is also necessary for the bactericidal activity of the properdin system. Maximal bactericidal activity is obtained with magnesium concentrations similar to that of normal human serum (10–3 to 10–4 M). The bactericidal activity of the properdin system occurs only at temperatures above 15°. Resistant strains have been encountered in species of bacteria sensitive to the properdin system. Resistance or sensitivity is a characteristic of the individual strain and not of the species. The widespread occurrence of the properdin system in normal mammalian serum and the variety of bacteria destroyed by it suggest that the properdin system is a factor in natural resistance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FRANK E., FINE J., PILLEMER L. Serum properdin levels in hemorrhagic shock. Proc Soc Exp Biol Med. 1955 Jun;89(2):223–225. doi: 10.3181/00379727-89-21764. [DOI] [PubMed] [Google Scholar]
  2. GINSBERG H. S., HORSFALL F. L., Jr A labile component of normal serum which combines with various viruses; neutralization of infectivity and inhibition of hemagglutination by the component. J Exp Med. 1949 Nov;90(5):475–495. doi: 10.1084/jem.90.5.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LEPOW I. H., WURZ L., RATNOFF O. D., PILLEMER L. Studies on the mechanism of inactivation of human complement by plasmin and by antigen-antibody aggregates. I. The requirement for a factor resembling C'1 and the role of Ca++. J Immunol. 1954 Sep;73(3):146–158. [PubMed] [Google Scholar]
  4. PILLEMER L., BLUM L., LEPOW I. H., ROSS O. A., TODD E. W., WARDLAW A. C. The properdin system and immunity. I. Demonstration and isolation of a new serum protein, properdin, and its role in immune phenomena. Science. 1954 Aug 20;120(3112):279–285. doi: 10.1126/science.120.3112.279. [DOI] [PubMed] [Google Scholar]
  5. PILLEMER L., BLUM L., LEPOW I. H., WURZ L., TODD E. W. The properdin system and immunity. III. The zymosan assay of properdin. J Exp Med. 1956 Jan 1;103(1):1–13. doi: 10.1084/jem.103.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. PILLEMER L., RATNOFF O. D., BLUM L., LEPOW I. H. The inactivation of complement and its components by plasmin. J Exp Med. 1953 Apr;97(4):573–589. doi: 10.1084/jem.97.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. PILLEMER L., SCHOENBERG M. D., BLUM L., WURZ L. Properdin system and immunity. II. Interaction of the properdin system with polysaccharides. Science. 1955 Sep 23;122(3169):545–549. doi: 10.1126/science.122.3169.545. [DOI] [PubMed] [Google Scholar]
  8. ROWLEY D. Stimulation of natural immunity to Escherichia coli infection: observations on mice. Lancet. 1955 Jan 29;268(6857):232–234. doi: 10.1016/s0140-6736(55)90163-x. [DOI] [PubMed] [Google Scholar]

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