Abstract
Studies of virulent, attenuated, and avirulent strains of tubercle bacilli have demonstrated the proficiency of virulent strains to effect degeneration of normal monocytes cultivated in the presence of normal serum. Attenuated strains were less active in this respect, and avirulent bacilli failed to induce monocytic degeneration. Comparison of the effects of virulent H37Rv with O.T., P.P.D., and a culture filtrate of H37Rv revealed a similarity in action of H37Rv and its filtrate. The action of O.T. and P.P.D. differed from that of H37Rv in that the greatest effect of H37Rv was upon normal cells as opposed to the effect of O.T. and P.P.D. upon immune cells. Additionally, it was demonstrated that immune serum (anti-BCG) protected immune cells against H37Rv but not against O.T. or P.P.D. The protection of immune cells by heterologous antisera (anti-Salmonella and anti-ovalbumin) as well as by homologous antiserum (anti-BCG) against the degenerative effects of H37Rv indicated a non-specificity in action of serum factors. The ability of the monocytes of animals immunized with BCG and the failure of monocytes of animals immunized with Salmonella rutgers to withstand parasitization with H37Rv, when both types of monocytes were cultivated in immune (anti-BCG) serum, indicated a specificity of cellular resistance.
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Selected References
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- Aronson J. D. THE SPECIFIC CYTOTOXIC ACTION OF TUBERCULIN IN TISSUE CULTURE. J Exp Med. 1931 Jul 31;54(3):387–397. doi: 10.1084/jem.54.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BLOCH H. Studies on the virulence of tubercle bacilli; isolation and biological properties of a constituent of virulent organisms. J Exp Med. 1950 Feb;91(2):197-218, pl. doi: 10.1084/jem.91.2.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BLOCH H. Studies on the virulence of tubercle bacilli; the relationship of the physiological state of the organisms to their pathogenicity. J Exp Med. 1950 Dec;92(6):507–526. doi: 10.1084/jem.92.6.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baldwin E. R. Studies on Tuberculous Serums and the Bacteriolysis of Bacillus Tuberculosis. J Med Res. 1904 Aug;12(2):215–233. [PMC free article] [PubMed] [Google Scholar]
- Choucroun N. BIOLOGICAL EFFECTS OF A TOXIC AND A SENSITIZING SUBSTANCE ISOLATED FROM PARAFFIN OIL EXTRACT OF DEAD TUBERCLE BACILLI. Science. 1943 Oct 8;98(2545):327–329. doi: 10.1126/science.98.2545.327. [DOI] [PubMed] [Google Scholar]
- ELBERG S. S., FONG J., SCHNEIDER P. Studies on tubercle bacillus-monocyte relationship. I. Quantitative analysis of effect of serum of animals vaccinated with BCG upon bacterium-monocyte system. J Exp Med. 1956 Oct 1;104(4):455–465. doi: 10.1084/jem.104.4.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACKANESS G. B., SMITH N., WELLS A. Q. The growth of intracellular tubercle bacilli in relation to their virulence. Am Rev Tuberc. 1954 Apr;69(4):479–494. doi: 10.1164/art.1954.69.4.479. [DOI] [PubMed] [Google Scholar]
- MACKANESS G. B. The action of drugs on intracellular tubercle bacilli. J Pathol Bacteriol. 1952 Jul;64(3):429–446. doi: 10.1002/path.1700640302. [DOI] [PubMed] [Google Scholar]
- Manwaring W. H., Bronfenbrenner J. INTRAPERITONEAL LYSIS OF TUBERCLE BACILLI. J Exp Med. 1913 Dec 1;18(6):601–617. doi: 10.1084/jem.18.6.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moen J. K. TISSUE CULTURE STUDIES ON BACTERIAL HYPERSENSITIVITY : III. THE PERSISTENCE IN VITRO OF THE INHERENT SENSITIVITY TO TUBERCULIN OF CELLS FROM TUBERCULOUS ANIMALS. J Exp Med. 1936 Nov 30;64(6):943–951. doi: 10.1084/jem.64.6.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PIERCE C. H., DUBOS R. J., SCHAEFER W. B. Multiplication and survival of tubercle bacilli in the organs of mice. J Exp Med. 1953 Feb 1;97(2):189–206. doi: 10.1084/jem.97.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pierce C., Dubos R. J., Middlebrook G. INFECTION OF MICE WITH MAMMALIAN TUBERCLE BACILLI GROWN IN TWEEN-ALBUMIN LIQUID MEDIUM. J Exp Med. 1947 Jul 31;86(2):159–174. doi: 10.1084/jem.86.2.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SPITZNAGEL J. K., DUBOS R. J. A fraction of tubercle bacilli possessing primary toxicity. J Exp Med. 1955 Mar 1;101(3):291–311. doi: 10.1084/jem.101.3.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SUTER E. The multiplication of tubercle bacilli within normal phagocytes in tissue culture. J Exp Med. 1952 Aug;96(2):137–150. doi: 10.1084/jem.96.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]