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. 1958 Feb 28;107(3):451–474. doi: 10.1084/jem.107.3.451

STUDIES ON THE ACTIVATION OF A PROESTERASE ASSOCIATED WITH PARTIALLY PURIFIED FIRST COMPONENT OF HUMAN COMPLEMENT

Irwin H Lepow 1, Oscar D Ratnoff 1, Lawrence R Levy 1
PMCID: PMC2136687  PMID: 13513912

Abstract

It has been found that under a wide range of physico-chemical conditions a positive correlation exists between the rate of disappearance of hemolytically active, partially purified first component of human complement and the rate of activation of an esterase hydrolyzing N-acetyl-L-tyrosine ethyl ester. Both reactions follow the kinetic equation for second order autocatalysis, with an apparent energy of activation of 31,000 calories per mol. They occur optimally at pH 7.3–7.7 and are inhibited by ionic strengths greater than 0.15, by 5 x 105 M ethylenediaminetetraacetic acid, and by a heat-labile serum inhibitor which appears unrelated to any component of complement. The activation of first component to esterase resembles closely the activation of trypsinogen to trypsin. Partially purified first component, containing plasminogen, may also be activated to esterase by addition of streptokinase. The significance of these data with respect to the postulated existence of first component as a proesterase and its possible role in complement-"fixation" is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AGGELER P. M., WHITE S. G., GLENDENING M. B., PAGE E. W., LEAKE T. B., BATES G. Plasma thromboplastin component (PTC) deficiency; a new disease resembling hemophilia. Proc Soc Exp Biol Med. 1952 Apr;79(4):692–694. doi: 10.3181/00379727-79-19488. [DOI] [PubMed] [Google Scholar]
  2. BECKER E. L. Concerning the mechanism of complement action. I. Inhibition of complement activity by diisopropyl fluophosphate. J Immunol. 1956 Dec;77(6):462–468. [PubMed] [Google Scholar]
  3. Bier O. G., Leyton G., Mayer M. M., Heidelberger M. A COMPARISON OF HUMAN AND GUINEA PIG COMPLEMENTS AND THEIR COMPONENT FRACTIONS. J Exp Med. 1945 May 1;81(5):449–468. doi: 10.1084/jem.81.5.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LEPOW I. H., PILLEMER L., RATNOFF O. D. The influence of calcium ions on the inactivation of human complement and its components by plasmin. J Exp Med. 1953 Sep;98(3):277–289. doi: 10.1084/jem.98.3.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LEPOW I. H., RATNOFF O. D., PILLEMER L. Elution of an esterase from antigen-antibody aggregates treated with human complement. Proc Soc Exp Biol Med. 1956 May;92(1):111–114. doi: 10.3181/00379727-92-22401. [DOI] [PubMed] [Google Scholar]
  6. LEPOW I. H., WURZ L., RATNOFF O. D., PILLEMER L. Studies on the mechanism of inactivation of human complement by plasmin and by antigen-antibody aggregates. I. The requirement for a factor resembling C'1 and the role of Ca++. J Immunol. 1954 Sep;73(3):146–158. [PubMed] [Google Scholar]
  7. LEVINE L. Inhibition of immune hemolysis by diisopropyl fluorophosphate. Biochim Biophys Acta. 1955 Oct;18(2):283–284. doi: 10.1016/0006-3002(55)90069-x. [DOI] [PubMed] [Google Scholar]
  8. PILLEMER L., BLUM L., LEPOW I. H., ROSS O. A., TODD E. W., WARDLAW A. C. The properdin system and immunity. I. Demonstration and isolation of a new serum protein, properdin, and its role in immune phenomena. Science. 1954 Aug 20;120(3112):279–285. doi: 10.1126/science.120.3112.279. [DOI] [PubMed] [Google Scholar]
  9. PILLEMER L., RATNOFF O. D., BLUM L., LEPOW I. H. The inactivation of complement and its components by plasmin. J Exp Med. 1953 Apr;97(4):573–589. doi: 10.1084/jem.97.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. RATNOFF O. D., COLOPY J. E. A familial hemorrhagic trait associated with a deficiency of a clot-promoting fraction of plasma. J Clin Invest. 1955 Apr;34(4):602–613. doi: 10.1172/JCI103109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RATNOFF O. D., LEPOW I. H. Some properties of an esterase derived from preparations of the first component of complement. J Exp Med. 1957 Aug 1;106(2):327–343. doi: 10.1084/jem.106.2.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. TROLL W., SHERRY S., WACHMAN J. The action of plasmin on synthetic substrates. J Biol Chem. 1954 May;208(1):85–93. [PubMed] [Google Scholar]

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