Abstract
Purified levan is antigenic in man and subcutaneous injection of 1 mg. leads to the production of precipitins and skin sensitivity. Apple amylopectin, maize glycogen, and synthetic polyglucose did not stimulate antibody production in small numbers of individuals injected. Quantitative precipitin studies carried out with several levan preparations employing human antilevan show variations in immunochemical behavior indicating structural differences among levans. Evidence that precipitins formed in response to levan injection are antilevan antibodies was obtained by analysis of specific precipitates formed in the region of antibody excess for ketosugar. Injection of levan giving rise to production of antilevan failed in one individual to give a non-specific anamnestic rise in either antidextran or antiblood group A precipitins. Laminarin, a neutral polysaccharide, was found to give precipitation with normal human sera.
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- BEISER S. B., KABAT E. A., SCHOR J. M. Immunochemical studies on the specific polysaccharide of type II Pneumococcus. J Immunol. 1952 Sep;69(3):297–309. [PubMed] [Google Scholar]
- BULL J. P., RICKETTS C. Dextran as a plasma substitute. Lancet. 1949 Jan 22;1(6543):134–143. doi: 10.1016/s0140-6736(49)90423-7. [DOI] [PubMed] [Google Scholar]
- Bendich A., Kabat E. A., Bezer A. E., With an Appendix by Erwin Brand, Ph.D., and Leo J. Saidel IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : III. PROPERTIES OF PURIFIED BLOOD GROUP A SUBSTANCES FROM INDIVIDUAL HOG STOMACH LININGS. J Exp Med. 1946 May 31;83(6):485–497. [PMC free article] [PubMed] [Google Scholar]
- DAVIES A. M., SHILO M., HESTRIN S. The influence of Aerobacter levan on the permeability of the blood vessels of the skin: studies with antibody globulins and trypan blue. Br J Exp Pathol. 1955 Oct;36(5):500–506. [PMC free article] [PubMed] [Google Scholar]
- Francis T., Tillett W. S. CUTANEOUS REACTIONS IN PNEUMONIA. THE DEVELOPMENT OF ANTIBODIES FOLLOWING THE INTRADERMAL INJECTION OF TYPE-SPECIFIC POLYSACCHARIDE. J Exp Med. 1930 Sep 30;52(4):573–585. doi: 10.1084/jem.52.4.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRABAR P. Reactions de divers sérums normaux avec des substances macromoléculaires naturelles ou synthétiques. Ann Inst Pasteur (Paris) 1955 Jan;88(1):11–23. [PubMed] [Google Scholar]
- HORSFALL F. L., Jr Studies on non-hemolytic streptococci isolated from the respiratory tract of man; the antigenic basis for type specific reactions with streptococcus salivarius and non-levan-forming streptococci. J Exp Med. 1951 Mar;93(3):229–245. doi: 10.1084/jem.93.3.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hehre E. J., Neill J. M. FORMATION OF SEROLOGICALLY REACTIVE DEXTRANS BY STREPTOCOCCI FROM SUBACUTE BACTERIAL ENDOCARDITIS. J Exp Med. 1946 Jan 31;83(2):147–162. [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Aisenberg A. C. Serological Reactivity of Synthetic Polyglucoses. Proc Natl Acad Sci U S A. 1953 Jun;39(6):453–459. doi: 10.1073/pnas.39.6.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Macleod C. M., Kaiser S. J., Robinson B. ANTIBODY FORMATION IN VOLUNTEERS FOLLOWING INJECTION OF PNEUMOCOCCI OR THEIR TYPE-SPECIFIC POLYSACCHARIDES. J Exp Med. 1946 Mar 31;83(4):303–320. [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Macpherson C. F. QUANTITATIVE MICRO-ESTIMATION OF ANTIBODIES IN THE SERA OF MAN AND OTHER ANIMALS. Science. 1943 Apr 30;97(2522):405–406. doi: 10.1126/science.97.2522.405. [DOI] [PubMed] [Google Scholar]
- Hestrin S., Avineri-Shapiro S., Aschner M. The enzymic production of levan. Biochem J. 1943 Oct;37(4):450–456. doi: 10.1042/bj0370450. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JOHNS R. G. S., MARRACK J. R. The reaction of H-substance with rabbit antisera. J Hyg (Lond) 1953 Mar;51(1):55–63. doi: 10.1017/s0022172400015497. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KABAT E. A., BAER H., KNAUB V. Immunochemical studies on blood groups; specific precipitation of the fucose of hog and human blood group A substances by anti-A. J Exp Med. 1949 Jan;89(1):1–10. doi: 10.1084/jem.89.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KABAT E. A., BERG D. Dextran; an antigen in man. J Immunol. 1953 Jun;70(6):514–532. [PubMed] [Google Scholar]
- Kabat E. A., Baer H., Day R. L., Knaub V. IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : XI. SPECIES DIFFERENCES AMONG BLOOD GROUP A SUBSTANCES. J Exp Med. 1950 Mar 31;91(4):433–445. doi: 10.1084/jem.91.4.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kabat E. A., Bezer A. E. IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : I. ESTIMATION OF A AND B ISOANTIBODIES IN HUMAN SERUM BY THE QUANTITATIVE PRECIPITIN METHOD. J Exp Med. 1945 Sep 1;82(3):207–215. doi: 10.1084/jem.82.3.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAURER P. H. Dextran, an antigen in man. Proc Soc Exp Biol Med. 1953 Aug-Sep;83(4):879–884. doi: 10.3181/00379727-83-20521. [DOI] [PubMed] [Google Scholar]
- MAURER P. H., LEBOVITZ H. Studies on the antigenicity of modified fluid gelatin. J Immunol. 1956 May;76(5):335–341. [PubMed] [Google Scholar]
- Stokinger H. E., Heidelberger M. A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VI. THE REACTION BETWEEN MAMMALIAN THYROGLOBULINS AND ANTIBODIES TO HOMOLOGOUS AND HETEROLOGOUS PREPARATIONS. J Exp Med. 1937 Jul 31;66(2):251–272. doi: 10.1084/jem.66.2.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILHAM C. A., ALEXANDER B. H., JEANES A. Heterogeneity in dextran preparations. Arch Biochem Biophys. 1955 Nov;59(1):61–75. doi: 10.1016/0003-9861(55)90463-x. [DOI] [PubMed] [Google Scholar]