Skip to main content
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1957 May 1;105(5):403–415. doi: 10.1084/jem.105.5.403

QUANTITATIVE STUDIES ON THE PROPERDIN-COMPLEMENT SYSTEM

II. KINETICS OF THE REACTION BETWEEN PROPERDIN AND ZYMOSAN

Myron A Leon 1; With the Technical Assistance of Edna Chapman and Arnold Ransby1
PMCID: PMC2136705  PMID: 13428911

Abstract

The reaction of human, bovine, and pig properdin with excess zymosan exhibits an induction period followed by a rapidly accelerating formation of the properdin-zymosan (PZ) complex. Two methods for determining the quantity of PZ complex are given, one using human serum lacking properdin, the other using a fraction of pig serum containing complement components, C'1, C'3 and C'4.

Full Text

The Full Text of this article is available as a PDF (448.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bier O. G., Leyton G., Mayer M. M., Heidelberger M. A COMPARISON OF HUMAN AND GUINEA PIG COMPLEMENTS AND THEIR COMPONENT FRACTIONS. J Exp Med. 1945 May 1;81(5):449–468. doi: 10.1084/jem.81.5.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HEIDELBERGER M., JONSEN J., WAKSMAN B. H., MANSKI W. Attempts at a quantitative estimation of the second component of complement. J Immunol. 1951 Dec;67(6):449–462. [PubMed] [Google Scholar]
  3. LEON M. A. Effect of temperature on activity of guinea pig complement. Proc Soc Exp Biol Med. 1956 Jan;91(1):150–152. [PubMed] [Google Scholar]
  4. LEON M. A. Kinetics of human complement. II. Separation of the reaction between human complement and sensitized cells into two steps. J Immunol. 1956 Jun;76(6):428–432. [PubMed] [Google Scholar]
  5. LEON M. A. Kinetics of human complement. Proc Soc Exp Biol Med. 1955 Aug;89(4):560–561. doi: 10.3181/00379727-89-21874. [DOI] [PubMed] [Google Scholar]
  6. LEON M. A., PLESCIA O. J., HEIDELBERGER M. The preparation and properties of fractions of pig complement. II. J Immunol. 1955 Apr;74(4):313–317. [PubMed] [Google Scholar]
  7. LEON M. A. Quantitative studies on the properdin-complement system. J Exp Med. 1956 Mar 1;103(3):285–293. doi: 10.1084/jem.103.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MAYER M. M., LEVINE L., RAPP H. J., MARUCCI A. A. Kinetic studies on immune hemolysis. VII. Decay of EAC'1, 4, 2, fixation of C'3, and other factors influencing the hemolytic action of complement. J Immunol. 1954 Dec;73(6):443–454. [PubMed] [Google Scholar]
  9. Mayer M. M., Osler A. G., Bier O. G., Heidelberger M. THE ACTIVATING EFFECT OF MAGNESIUM AND OTHER CATIONS ON THE HEMOLYTIC FUNCTION OF COMPLEMENT. J Exp Med. 1946 Nov 30;84(6):535–548. doi: 10.1084/jem.84.6.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. OSLER A. G., STRAUSS J. H., MAYER M. M. Diagnostic complement fixation. I. A method. Am J Syph Gonorrhea Vener Dis. 1952 Mar;36(2):140–153. [PubMed] [Google Scholar]
  11. PILLEMER L., BLUM L., LEPOW I. H., ROSS O. A., TODD E. W., WARDLAW A. C. The properdin system and immunity. I. Demonstration and isolation of a new serum protein, properdin, and its role in immune phenomena. Science. 1954 Aug 20;120(3112):279–285. doi: 10.1126/science.120.3112.279. [DOI] [PubMed] [Google Scholar]
  12. PILLEMER L., BLUM L., LEPOW I. H., WURZ L., TODD E. W. The properdin system and immunity. III. The zymosan assay of properdin. J Exp Med. 1956 Jan 1;103(1):1–13. doi: 10.1084/jem.103.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PILLEMER L., SCHOENBERG M. D., BLUM L., WURZ L. Properdin system and immunity. II. Interaction of the properdin system with polysaccharides. Science. 1955 Sep 23;122(3169):545–549. doi: 10.1126/science.122.3169.545. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES