Abstract
By maintaining L cells in a balanced salt solution of inorganic salts and glucose (BSS) for 2 days or more, they are rendered incapable of supporting the growth of psittacosis virus (6BC), though it infects such cells and is present intracellularly for as long as 3 days in a non-infectious phase. The addition of an enriched medium to such a culture of cells at any time up to 4 days after infection results in the appearance of infectious virus within these cells, which multiplies and is released from the cells, providing the entire period of exposure of such cells to the BSS does not exceed 6 days, following which the cells die. A latent infection with psittacosis virus in a non-infectious phase has been established in a pure line of cells which possess properties of malignancy.
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Selected References
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- DULBECCO R. Interaction of viruses and animal cells; a study of facts and interpretations. Physiol Rev. 1955 Apr;35(2):301–335. doi: 10.1152/physrev.1955.35.2.301. [DOI] [PubMed] [Google Scholar]
- EAGLE H. The specific amino acid requirements of a mammalian cell (strain L) in tissue culture. J Biol Chem. 1955 Jun;214(2):839–852. [PubMed] [Google Scholar]
- EARLE W., HIGHHOUSE F. Culture flasks for use with plane surface substrate tissue cultures. J Natl Cancer Inst. 1954 Feb;14(4):841–851. [PubMed] [Google Scholar]
- HARE J. D., MORGAN H. R. Studies on the factors essential to the initiation and maintenance of multiplication of psittacosis virus (6BC strain) in deficient cells in tissue culture. J Exp Med. 1954 May 1;99(5):461–479. doi: 10.1084/jem.99.5.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HEGGIE A. D., MORGAN H. R. Latent viral infection of cells in tissue culture. III. Role of certain amino acids. Proc Soc Exp Biol Med. 1956 Jul;92(3):506–509. doi: 10.3181/00379727-92-22526. [DOI] [PubMed] [Google Scholar]
- JOHNSON K. M., MORGAN H. R. Latent viral infection of cells in tissue culture. II. Relationship of cell nutrition to initiation of growth of psittacosis virus. J Exp Med. 1956 Jun 1;103(6):765–775. doi: 10.1084/jem.103.6.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MORGAN H. R. Latent viral infection of cells in tissue culture. I. Studies on latent infection of chick embryo tissues with psittacosis virus. J Exp Med. 1956 Jan 1;103(1):37–47. doi: 10.1084/jem.103.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SANFORD K. K., HOBBS G. L., EARLE W. R. The tumor-producing capacity of strain L mouse cells after 10 years in vitro. Cancer Res. 1956 Feb;16(2):162–166. [PubMed] [Google Scholar]