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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1958 May 1;107(5):643–652. doi: 10.1084/jem.107.5.643

THE AMINO ACID REQUIREMENTS OF MONKEY KIDNEY CELLS IN FIRST CULTURE PASSAGE

Harry Eagle 1, A E Freeman 1, M Levy 1
PMCID: PMC2136844  PMID: 13525576

Abstract

Monkey kidney cells tested in their first culture passage, 24 hours after their isolation from the animal host, required the same 13 amino acids for survival and growth as cell lines serially propagated in culture for years. Under the conditions of the present experiments, arginine, cystine, glutamine, histidine, and tyrosine proved necessary, over and above the 8 amino acids required for nitrogen balance in man. With the serially propagated lines, glutamic acid substituted for glutamine only at extremely high and non-physiological levels. In the monkey kidney cell cultures, however, glutamic acid and glutamine were interchangeable, mole for mole; and aspartic acid and asparagine were also effective as glutamine substitutes. Glycine was growth-stimulatory for monkey kidney cells in primary culture, and the cells grown in a glycine-deficient medium usually failed to survive subculture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EAGLE H., OYAMA V. I., LEVY M. Amino acid requirements of normal and malignant human cells in tissue culture. Arch Biochem Biophys. 1957 Apr;67(2):432–446. doi: 10.1016/0003-9861(57)90298-9. [DOI] [PubMed] [Google Scholar]
  2. EAGLE H., OYAMA V. I., LEVY M., FREEMAN A. E. Myo-Inositol as an essential growth factor for normal and malignant human cells in tissue culture. J Biol Chem. 1957 May;226(1):191–205. [PubMed] [Google Scholar]
  3. EAGLE H., OYAMA V. I., LEVY M., HORTON C. L., FLEISCHMAN R. The growth response of mammalian cells in tissue culture to L-glutamine and L-glutamic acid. J Biol Chem. 1956 Feb;218(2):607–616. [PubMed] [Google Scholar]
  4. EAGLE H. The specific amino acid requirements of a mammalian cell (strain L) in tissue culture. J Biol Chem. 1955 Jun;214(2):839–852. [PubMed] [Google Scholar]
  5. MAXWELL M., MCCOY T. A., NEUMAN R. E. The amino acid requirements of the Walker carcinosarcoma 256 in vitro. Cancer Res. 1956 Nov;16(10 Pt 1):979–984. [PubMed] [Google Scholar]
  6. OYAMA V. I., EAGLE H. Measurement of cell growth in tissue culture with a phenol reagent (folin-ciocalteau). Proc Soc Exp Biol Med. 1956 Feb;91(2):305–307. doi: 10.3181/00379727-91-22245. [DOI] [PubMed] [Google Scholar]
  7. ROSE W. C., WIXOM R. L., LOCKHART H. B., LAMBERT G. F. The amino acid requirements of man. XV. The valine requirement; summary and final observations. J Biol Chem. 1955 Dec;217(2):987–995. [PubMed] [Google Scholar]

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