Abstract
Brucella suis, Brucella abortus, and Brucella melitensis were shown by microscopic and cultural procedures to multiply extensively within normal rat, mouse, and guinea pig monocytes maintained in vitro in cell cultures for 3 days. Intracellular growth of brucellae had no observable toxic effects on most monocytes, although many of the cells became completely engorged with brucellae within 3 days. Non-smooth brucellae and strain 19 multiplied slowly within normal monocytes. In contrast, "immune" monocytes) i.e. those derived from animals previously infected with smooth brucellae, greatly restricted the intracellular growth of smooth and non-smooth brucellae and strain 19. Growth of smooth Brucella, within either normal or "immune" monocytes, was not influenced by addition of Brucella antiserum to the culture medium. Desensitization of immunized guinea pigs did not diminish the refractory state of their monocytes. Cellular resistance did not develop when animals were vaccinated with heat-killed brucellae, though these animals did produce agglutinating antibody. Similarly, vaccination of animals with living, rough B. suis failed to induce a refractory state in their monocytes, even though the vaccinated animals developed delayed hypersensitivity to smooth Brucella antigen. In vivo studies of Brucella survival in the spleens of normal and vaccinated mice (treated with streptomycin to prevent extracellular survival) gave strong support to the in vitro demonstrations of acquired "cellular immunity." Some implications of these results are discussed.
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Selected References
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- BARSKI G., LAMY M., LEPINE P. Culture de cellules trypsinées de rein de lapin et leur application a l'étude des virus du groupe herpétique. Ann Inst Pasteur (Paris) 1955 Oct;89(4):415–427. [PubMed] [Google Scholar]
- BARSKI G., MESSORE G., LEPINE P. L'exsudat péritonéal artificiel, source de cellules pour la culture des virus in vitro. Ann Inst Pasteur (Paris) 1955 Sep;89(3):366–371. [PubMed] [Google Scholar]
- BERMAN D. T., REDFEARN M. S., SIMON E. M. The establishment of colonial variants in guinea pigs infected with smooth Brucella suis. J Infect Dis. 1956 Nov-Dec;99(3):234–240. doi: 10.1093/infdis/99.3.234. [DOI] [PubMed] [Google Scholar]
- BRAUDE A. I. Studies in the pathology and pathogenesis of experimental brucellosis. II. The formation of the hepatic granuloma and its evolution. J Infect Dis. 1951 Jul-Aug;89(1):87–94. doi: 10.1093/infdis/89.1.87. [DOI] [PubMed] [Google Scholar]
- BRAUN W., POMALES-LEBRON A., STINEBRING W. R. Interactions between mononuclear phagocytes and Brucella abortus strains of different virulence. Proc Soc Exp Biol Med. 1958 Feb;97(2):393–397. doi: 10.3181/00379727-97-23752. [DOI] [PubMed] [Google Scholar]
- BRAUN W. Studies on bacterial variation and selective environments; the effects of sera from brucella-infected animals and from normal animals of different species upon the variation of Brucella abortus. J Bacteriol. 1949 Sep;58(3):299–305. doi: 10.1128/JB.58.3.299-305.1949. [DOI] [PubMed] [Google Scholar]
- DUBOS R. J., SCHAEDLER R. W. Effects of cellular constituents of mycobacteria on the resistance of mice to heterologous infections I. Protective effects. J Exp Med. 1957 Nov 1;106(5):703–717. doi: 10.1084/jem.106.5.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ELBERG S. S., SCHNEIDER P., FONG J. Cross-immunity between Brucella melitensis and Mycobacterium tuberculosis; intracellular behavior of Brucella melitensis in monocytes from vaccinated animals. J Exp Med. 1957 Oct 1;106(4):545–554. doi: 10.1084/jem.106.4.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodpasture E. W., Anderson K. The Problem of Infection as Presented by Bacterial Invasion of the Chorio-Allantoic Membrane of Chick Embryos. Am J Pathol. 1937 Mar;13(2):149–174.7. [PMC free article] [PubMed] [Google Scholar]
- HOLLAND J. J., PICKETT M. J. Intracellular behavior of Brucella variants in chick embryo cells in tissue culture. Proc Soc Exp Biol Med. 1956 Dec;93(3):476–479. doi: 10.3181/00379727-93-22792. [DOI] [PubMed] [Google Scholar]
- JONES L. M. Further studies of the pathogenicity and immunogenicity of mucoid variants of Brucella abortus for guinea pigs. J Infect Dis. 1953 Jan-Feb;92(1):26–32. doi: 10.1093/infdis/92.1.26. [DOI] [PubMed] [Google Scholar]
- Lurie M. B., Collaboration of Peter Zappasodi STUDIES ON THE MECHANISM OF IMMUNITY IN TUBERCULOSIS : THE FATE OF TUBERCLE BACILLI INGESTED BY MONONUCLEAR PHAGOCYTES DERIVED FROM NORMAL AND IMMUNIZED ANIMALS. J Exp Med. 1942 Mar 1;75(3):247–268. doi: 10.1084/jem.75.3.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACKANESS G. B. The growth of tubercle bacilli in monocytes from normal and vaccinated rabbits. Am Rev Tuberc. 1954 Apr;69(4):495–504. doi: 10.1164/art.1954.69.4.495. [DOI] [PubMed] [Google Scholar]
- NYKA W., FAHERTY J. F., MALONE L. C., KISER J. S. A histological study of the pathogenesis of tuberculosis in mice experimentally infected with bacilli of human type. Exp Med Surg. 1954;12(3):367–429. [PubMed] [Google Scholar]
- SCHAEDLER R. W., DUBOS R. J. Effects of cellular constituents of mycobacteria on the resistance of mice to heterologous infections. II. Enhancement of infection. J Exp Med. 1957 Nov 1;106(5):719–726. doi: 10.1084/jem.106.5.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SIMON E. M., REDFEARN M. S., BERMAN D. T. Competitive values of smooth and mucoid Brucella abortus in mixed infections in mice. J Infect Dis. 1955 May-Jun;96(3):268–278. doi: 10.1093/infdis/96.3.268. [DOI] [PubMed] [Google Scholar]