Skip to main content
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1958 Jul 1;108(1):121–130. doi: 10.1084/jem.108.1.121

THE FORMATION OF MYELOMA PROTEIN BY A MOUSE PLASMA CELL TUMOR

Daniel Nathans 1, John L Fahey 1, Michael Potter 1
PMCID: PMC2136887  PMID: 13549645

Abstract

The origin of the myeloma protein found in mice bearing the plasma cell tumor X5563 has been investigated. Specific activity-time curves of the myeloma proteins isolated from the tumor and from the plasma of these animals were compared following intravenous injection of L-lysine-C14. The results indicate that myeloma protein is synthesized in the plasma cell tumor.

Full Text

The Full Text of this article is available as a PDF (487.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLACKBURN S., LOWTHER A. G. The separation of N-2:4-dinitrophenly amino-acids on paper chromatograms. Biochem J. 1951 Jan;48(1):126–128. doi: 10.1042/bj0480126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BORSOOK H., DEASY C. L., HAAGENSMIT A. J., KEIGHLEY G., LOWY P. H. Metabolism of C14 labeled glycine, L-histidine, L-leucine, and L-lysine. J Biol Chem. 1950 Dec;187(2):839–848. [PubMed] [Google Scholar]
  3. FAHEY J. L., McCOY P. F., GOULIAN M. Chromatography of serum proteins in normal and pathologic sera: the distribution of protein-bound carbohydrate and cholesterol, siderophilin, thyroxin-binding protein, B12-binding protein, alkaline and acid phosphatases, radio-iodinated albumin and myeloma proteins. J Clin Invest. 1958 Feb;37(2):272–284. doi: 10.1172/JCI103606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FRAENKEL-CONRAT H., HARRIS J. I., LEVY A. L. Recent developments in techniques for terminal and sequence studies in peptides and proteins. Methods Biochem Anal. 1955;2:359–425. doi: 10.1002/9780470110188.ch12. [DOI] [PubMed] [Google Scholar]
  5. GREEN H., ANKER H. S. Kinetics of amino acid incorporation into serum proteins. J Gen Physiol. 1955 Jan 20;38(3):283–293. doi: 10.1085/jgp.38.3.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goodwin T. W., Morton R. A. The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochem J. 1946;40(5-6):628–632. doi: 10.1042/bj0400628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JENSEN D., TARVER H. Protein synthesis in the perfused rat liver. J Gen Physiol. 1956 Mar 20;39(4):567–583. doi: 10.1085/jgp.39.4.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MILLER L. L., BLY C. G., WATSON M. L., BALE W. F. The dominant role of the liver in plasma protein synthesis; a direct study of the isolated perfused rat liver with the aid of lysine-epsilon-C14. J Exp Med. 1951 Nov;94(5):431–453. doi: 10.1084/jem.94.5.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. POTTER M., FAHEY J. L., PILGRIM H. I. Abnormal serum protein and bone destruction in transmissible mouse plasma cell neoplasm (multiple myeloma). Proc Soc Exp Biol Med. 1957 Feb;94(2):327–333. doi: 10.3181/00379727-94-22035. [DOI] [PubMed] [Google Scholar]
  10. RUNDLES R. W., DILLON M. L., DILLON E. S. Multiple myeloma. III. Effect of urethane therapy on plasma cell growth, abnormal serum protein components and Bence Jones proteinuria. J Clin Invest. 1950 Sep;29(9):1243–1260. doi: 10.1172/JCI102362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SOONS J. B. Localization of pathological protein synthesis in multiple myelomatosis. Acta Physiol Pharmacol Neerl. 1956 Jul;5(1):114–114. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES