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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1958 Sep 30;108(4):475–491. doi: 10.1084/jem.108.4.475

ACCESSORY PLASMA FACTORS INVOLVED IN THE BACTERICIDAL TEST FOR TYPE-SPECIFIC ANTIBODY TO GROUP A STREPTOCOCCI

I. ATYPICAL BEHAVIOR OF SOME HUMAN AND RABBIT BLOODS

G H Stollerman 1, F S Kantor 1, B D Gordon 1
PMCID: PMC2136903  PMID: 13575679

Abstract

The bloods of two apparently healthy human beings, of 25 studied, failed to produce a strong bactericidal test for type-specific antibody to the M protein of group A streptococci under in vitro conditions wherein most human blood leukocytes rapidly phagocytize and destroy virulent organisms in the presence of anti-M antibody and accessory plasma factors. The defect in bactericidal activity of these two individuals is associated with the plasma rather than with the blood leukocytes. Leukocytes suspended in these atypical plasmas showed a characteristic delay in the rate of activation of phagocytosis. Although previously the bloods of laboratory animals (except monkeys) had been reported to be much less active than human blood in this system, occasional exceptions were encountered in rabbits in this study. Two rabbits were found whose bloods were as strongly bactericidal against streptococci, in the presence of type-specific antibody, as the blood of the average "normal" human being. The atypical behavior of some human and rabbit bloods in the bactericidal test may be explained by variations in accessory plasma factors that are as yet unidentified and that influence the rate of phagocytosis of virulent streptococci in vitro in the presence of type-specific antibody.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DENNY F. W., Jr, PERRY W. D., WANNAMAKER L. W. Type-specific streptococcal antibody. J Clin Invest. 1957 Jul;36(7):1092–1100. doi: 10.1172/JCI103504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FLECK D. G. Mouse protection and enhancement of phagocytosis by antisera to Streptococcus pyogenes. Br J Exp Pathol. 1956 Aug;37(4):406–414. [PMC free article] [PubMed] [Google Scholar]
  3. Kuttner A. G., Lenert T. F. THE OCCURRENCE OF BACTERIOSTATIC PROPERTIES IN THE BLOOD OF PATIENTS AFTER RECOVERY FROM STREPTOCOCCAL PHARYNGITIS. J Clin Invest. 1944 Mar;23(2):151–161. doi: 10.1172/JCI101478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LANCEFIELD R. C. Differentiation of group A streptococci with a common R antigen into three serological types, with special reference to the bactericidal test. J Exp Med. 1957 Oct 1;106(4):525–544. doi: 10.1084/jem.106.4.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MAXTED W. R. The indirect bactericidal test as a means of identifying antibody to the M antigen of Streptococcus pyogenes. Br J Exp Pathol. 1956 Aug;37(4):415–422. [PMC free article] [PubMed] [Google Scholar]
  6. Rothbard S. BACTERIOSTATIC EFFECT OF HUMAN SERA ON GROUP A STREPTOCOCCI : II. COMPARATIVE BACTERIOSTATIC EFFECT OF NORMAL WHOLE BLOOD FROM DIFFERENT ANIMAL SPECIES IN THE PRESENCE OF HUMAN CONVALESCENT SERA. J Exp Med. 1945 Aug 1;82(2):107–118. doi: 10.1084/jem.82.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. SKARNES R. C., WATSON D. W. Antimicrobial factors of normal tissues and fluids. Bacteriol Rev. 1957 Dec;21(4):273–294. doi: 10.1128/br.21.4.273-294.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. STOLLERMAN G. H., EKSTEDT R. Long chain formation by strains of group A streptococci in the presence of homologous antiserum: a type-specific reaction. J Exp Med. 1957 Sep 1;106(3):345–356. doi: 10.1084/jem.106.3.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Swift H. F., Wilson A. T., Lancefield R. C. TYPING GROUP A HEMOLYTIC STREPTOCOCCI BY M PRECIPITIN REACTIONS IN CAPILLARY PIPETTES. J Exp Med. 1943 Aug 1;78(2):127–133. doi: 10.1084/jem.78.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]

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