Abstract
Mouse fibroblasts (L cells) fail to support the growth of psittacosis virus (6BC strain) if they are maintained on a medium containing only inorganic salts and glucose for 2 days prior to infection. Virus propagation can be stimulated by the addition of a synthetic medium containing amino acids, water-soluble vitamins, glutamine, glucose, and inorganic salts. By omitting single amino acids from the complete synthetic medium, tyrosine, threonine, methionine, isoleucine, phenylalanine, tryptophan, leucine, valine, and cysteine or cystine were found to be essential for stimulation, while lysine, arginine, histidine, hydroxyproline, proline, glutamic acid, aspartic acid, serine, alanine, and glycine were not essential. The cells on deficient media showed varying degrees of degenerative changes, but there was little correlation between ability to support psittacosis virus growth and morphologic condition of the cells. Glucose is also an essential component of the medium for viral growth, but the absence of glutamine had no effect on stimulation of virus propagation. L cell cultures maintained on media deficient in phenylalanine or tryptophan for 2 days before infection were also found to be incapable of supporting virus growth. The implications of this study in latent viral infections are discussed.
Full Text
The Full Text of this article is available as a PDF (887.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALLEN E. G., BOVARNICK M. R. Association of reduced diphosphopyridine nucleotide cytochrome c reductase activity with meningopneumonitis virus. J Exp Med. 1957 Jun 1;105(6):539–547. doi: 10.1084/jem.105.6.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EAGLE H., HABEL K. The nutritional requirements for the propagation of poliomyelitis virus by the HeLa cell. J Exp Med. 1956 Aug 1;104(2):271–287. doi: 10.1084/jem.104.2.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EATON M. D. Observation on growth of virus and the energy-yielding activities of the host cell. Arch Gesamte Virusforsch. 1952;5(1):53–72. doi: 10.1007/BF01245139. [DOI] [PubMed] [Google Scholar]
- HEALY G. M., FISHER D. C., PARKER R. C. Nutrition of animal cells in tissue culture. IX. Synthetic medium No. 703. Can J Biochem Physiol. 1954 May;32(3):327–337. [PubMed] [Google Scholar]
- HEGGIE A. D., MORGAN H. R. Latent viral infection of cells in tissue culture. III. Role of certain amino acids. Proc Soc Exp Biol Med. 1956 Jul;92(3):506–509. doi: 10.3181/00379727-92-22526. [DOI] [PubMed] [Google Scholar]
- JOHNSON K. M., MORGAN H. R. Latent viral infection of cells in tissue culture. II. Relationship of cell nutrition to initiation of growth of psittacosis virus. J Exp Med. 1956 Jun 1;103(6):765–775. doi: 10.1084/jem.103.6.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUWATA T., SHIBA S. Influence of glucose on the multiplication of ornithosis virus in vitro. Experientia. 1955 Jul 15;11(7):269–269. doi: 10.1007/BF02161249. [DOI] [PubMed] [Google Scholar]
- MORGAN H. R. Factors related to the growth of psittacosis virus (strain 6BC). IV. Certain amino acids, vitamins, and other substances. J Exp Med. 1954 May 1;99(5):451–460. doi: 10.1084/jem.99.5.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MORGAN H. R. Studies on the relationship of pteroylglutamic acid to the growth of psittacosis virus, strain 6BC. J Exp Med. 1948 Sep 1;88(3):285–294. doi: 10.1084/jem.88.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]