Abstract
A methodology designed to eliminate mitotic inhibitor action and involving use of pretested fetal calf serum and careful pH and temperature control has been described by which cells from normal human and animal tissue can be maintained in active growth for long periods in vitro without development of aneuploidy. By means of this procedure, it is possible reliably to establish cell cultures from minute skin biopsies which can be taken from any individual. Clones of mammalian cells with chromosomal markers have been isolated by this means from x-irradiated non-irradiated cell cultures. Application of these techniques to chromosome delineation in large numbers of human subjects; determination of chromosomal sex in patients; spontaneuos and induced genetic changes in somatic mammalian cells in vivo and in vitro; comparison of metabolic differences between normal and cancerous cells and other problems have been indicated.
Full Text
The Full Text of this article is available as a PDF (834.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARR M. L., BERTRAM E. G. A morphological distinction between neurones of the male and female, and the behaviour of the nucleolar satellite during accelerated nucleoprotein synthesis. Nature. 1949 Apr 30;163(4148):676–676. doi: 10.1038/163676a0. [DOI] [PubMed] [Google Scholar]
- GEY G. O. Some aspects of the constitution and behavior of normal and malignant cells maintained in continuous culture. Harvey Lect. 1954;50:154–229. [PubMed] [Google Scholar]
- MOORE A. E., SOUTHAM C. M., STERNBERG S. S. Neoplastic changes developing in epithelial cell lines derived from normal persons. Science. 1956 Jul 20;124(3212):127–129. doi: 10.1126/science.124.3212.127-a. [DOI] [PubMed] [Google Scholar]
- PUCK T. T., CIECIURA S. J., FISHER H. W. Clonal growth in vitro of human cells with fibroblastic morphology; comparison of growth and genetic characteristics of single epithelioid and fibroblast-like cells from a variety of human organs. J Exp Med. 1957 Jul 1;106(1):145–158. doi: 10.1084/jem.106.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PUCK T. T., MARCUS P. I., CIECIURA S. J. Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer. J Exp Med. 1956 Feb 1;103(2):273–283. doi: 10.1084/jem.103.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Puck T. T. ACTION OF RADIATION ON MAMMALIAN CELLS III. RELATIONSHIP BETWEEN REPRODUCTIVE DEATH AND INDUCTION OF CHROMOSOME ANOMALIES BY X-IRRADIATION OF EUPLOID HUMAN CELLS IN VITRO. Proc Natl Acad Sci U S A. 1958 Aug 15;44(8):772–780. doi: 10.1073/pnas.44.8.772. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Puck T. T., Fisher H. W. GENETICS OF SOMATIC MAMMALIAN CELLS : I. DEMONSTRATION OF THE EXISTENCE OF MUTANTS WITH DIFFERENT GROWTH REQUIREMENTS IN A HUMAN CANCER CELL STRAIN (HELA). J Exp Med. 1956 Sep 1;104(3):427–434. doi: 10.1084/jem.104.3.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sato G., Fisher H. W., Puck T. T. Molecular Growth Requirements of Single Mammalian Cells. Science. 1957 Nov 8;126(3280):961–964. doi: 10.1126/science.126.3280.961. [DOI] [PubMed] [Google Scholar]
- THOMSON R. Y., FRAZER S. C. The deoxyribonucleic acid content of individual rat cell nuclei. Exp Cell Res. 1954 May;6(2):367–383. doi: 10.1016/0014-4827(54)90185-8. [DOI] [PubMed] [Google Scholar]
- TJIO J. H., PUCK T. T. Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. J Exp Med. 1958 Aug 1;108(2):259–268. doi: 10.1084/jem.108.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]