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. 1987 Sep;169(9):3945–3951. doi: 10.1128/jb.169.9.3945-3951.1987

Membrane-murein attachment at the leading edge of the division septum: a second membrane-murein structure associated with morphogenesis of the gram-negative bacterial division septum.

T J MacAlister, W R Cook, R Weigand, L I Rothfield
PMCID: PMC213692  PMID: 3305476

Abstract

Electron microscopy of plasmolyzed cells of Salmonella typhimurium revealed a continuous zone of membrane-murein attachment at the leading edge of the division septum at all stages of septal invagination. The membrane-murein attachment site had a characteristic ultrastructural appearance and remained as a bacterial birth scar at the new pole of each of the two daughter cells after cell separation. The continuous zone of membrane-murein attachment at the leading septal edge represents the second organelle based on a topologically ordered domain of membrane-murein adhesion to be described at the site of cell division.

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Selected References

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  1. Bayer M. E. Areas of adhesion between wall and membrane of Escherichia coli. J Gen Microbiol. 1968 Oct;53(3):395–404. doi: 10.1099/00221287-53-3-395. [DOI] [PubMed] [Google Scholar]
  2. Burdett I. D., Murray R. G. Electron microscope study of septum formation in Escherichia coli strains B and B-r during synchronous growth. J Bacteriol. 1974 Sep;119(3):1039–1056. doi: 10.1128/jb.119.3.1039-1056.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cook W. R., MacAlister T. J., Rothfield L. I. Compartmentalization of the periplasmic space at division sites in gram-negative bacteria. J Bacteriol. 1986 Dec;168(3):1430–1438. doi: 10.1128/jb.168.3.1430-1438.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fung J. C., MacAlister T. J., Weigand R. A., Rothfield L. I. Morphogenesis of the bacterial division septum: identification of potential sites of division in lkyD mutants of Salmonella typhimurium. J Bacteriol. 1980 Aug;143(2):1019–1024. doi: 10.1128/jb.143.2.1019-1024.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gilleland H. E., Jr, Murray R. G. Demonstration of cell division by septation in a variety of gram-negative rods. J Bacteriol. 1975 Feb;121(2):721–725. doi: 10.1128/jb.121.2.721-725.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MacAlister T. J., Costerton J. W., Thompson L., Thompson J., Ingram J. M. Distribution of alkaline phosphatase within the periplasmic space of gram-negative bacteria. J Bacteriol. 1972 Sep;111(3):827–832. doi: 10.1128/jb.111.3.827-832.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Macalister T. J., Macdonald B., Rothfield L. I. The periseptal annulus: An organelle associated with cell division in Gram-negative bacteria. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1372–1376. doi: 10.1073/pnas.80.5.1372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Weigand R. A., Vinci K. D., Rothfield L. I. Morphogenesis of the bacterial division septum: a new class of septation-defective mutants. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1882–1886. doi: 10.1073/pnas.73.6.1882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wetzel B. K., Spicer S. S., Dvorak H. F., Heppel L. A. Cytochemical localization of certain phosphatases in Escherichia coli. J Bacteriol. 1970 Oct;104(1):529–542. doi: 10.1128/jb.104.1.529-542.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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