Abstract
When one or two drops of a dilute, non-irritating solution of 2,4-dinitrochlorobenzene (DNCB) is applied to a small area of skin of the intact guinea pig, about 20 per cent of the applied material, or some derivative of it, is soon excreted in urine. In normal, as well as in specifically sensitized guinea pigs, DNCB at the site of local application becomes rapidly bound to skin protein through primary chemical bonds. Twenty-four hours after application roughly half of the material present at the local skin site is still extractable with organic solvents. Of the non-extractable dinitrophenyl groups, about 99 per cent are in epidermis, and about 85 per cent are substituted in ε-NH2 groups of lysine residues. Only traces of bound dinitrophenyl groups were observed in the corium. It is uncertain whether these are formed in situ, or are experimental contaminants, or are migratory epidermally formed conjugates. Even when DNCB is injected intradermally it combines predominantly with overlying epidermis and with epidermal components of hair follicles, but only slightly with corium. The 2,4-dinitrophenyl conjugates which are localized in the deeper, viable half of the epidermis, close to the epidermal-dermal junction, are inferred to be the agents responsible for specifically evoking the allergic response in sensitized animals. Conjugates which are situated in the outer, cornified half of the epidermis are shown to be incapable of eliciting the allergic response. The results furnish a basis for interpreting a common pattern of lesions in allergic contact dermatitis as it occurs spontaneously in man.
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Selected References
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- DANIELLI J. F. Studies on the cytochemistry of proteins. Cold Spring Harb Symp Quant Biol. 1950;14:32–39. doi: 10.1101/sqb.1950.014.01.006. [DOI] [PubMed] [Google Scholar]
- Dienes L., Mallory T. B. Histological Studies of Hypersensitive Reactions. Am J Pathol. 1932 Nov;8(6):689–710.3. [PMC free article] [PubMed] [Google Scholar]
- EISEN H. N., CARSTEN M. E., BELMAN S. Studies of hypersensitivity to low molecular weight substances. III. The 2,4-dinitrophenyl group as a determinant in the preciptin reaction. J Immunol. 1954 Nov;73(5):296–308. [PubMed] [Google Scholar]
- EISEN H. N. Delayed allergic skin responses of the contact dermatitis type. Bull N Y Acad Med. 1956 Mar;32(3):239; discussion, 240-3. [PubMed] [Google Scholar]
- EISEN H. N., ORRIS L., BELMAN S. Elicitation of delayed allergic skin reactions with haptens; the dependence of elicitation on hapten combination with protein. J Exp Med. 1952 May 1;95(5):473–487. doi: 10.1084/jem.95.5.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LI C. H., ASH L. The nitrogen terminal end-groups of hypophyseal growth hormone. J Biol Chem. 1953 Jul;203(1):419–424. [PubMed] [Google Scholar]
- Landsteiner K., Jacobs J. STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS. J Exp Med. 1935 Apr 30;61(5):643–656. doi: 10.1084/jem.61.5.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MIDDLEBROOK W. R. The chain weight of wool keratin. Biochim Biophys Acta. 1951 Nov;7(4):547–562. doi: 10.1016/0006-3002(51)90068-6. [DOI] [PubMed] [Google Scholar]
- PAPPENHEIMER A. M., Jr Hypersensitivity of the delayed type. Harvey Lect. 1956;(SERIES):100–118. [PubMed] [Google Scholar]
- PAPPENHEIMER A. M., SALVIN S. B., UHR J. W. Delayed hypersensitivity. II. Induction of hypersensitivity in guinea pigs by means of antigen-antibody complexes. J Exp Med. 1957 Jan 1;105(1):11–24. doi: 10.1084/jem.105.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porter R. R., Sanger F. The free amino groups of haemoglobins. Biochem J. 1948;42(2):287–294. doi: 10.1042/bj0420287. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAFFEL S., ARNAUD L. E. The role of the wax of the tubercle bacillus in establishing delayed hypersensitivity, hypersensitivity to a protein antigen, egg albumin. J Exp Med. 1949 Jul;90(1):53–72. doi: 10.1084/jem.90.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHEELDON L. W., COLLINS F. D. Studies on phospholipids. I. The determination of amino nitrogen in unhydrolysed phospholipids. Biochem J. 1957 Jul;66(3):435–441. doi: 10.1042/bj0660435. [DOI] [PMC free article] [PubMed] [Google Scholar]