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. 1958 Nov 30;108(6):803–821. doi: 10.1084/jem.108.6.803

STUDIES ON THE BACTERIOPHAGES OF HEMOLYTIC STREPTOCOCCI

II. ANTIGENS RELEASED FROM THE STREPTOCOCCAL CELL WALL BY A PHAGE-ASSOCIATED LYSIN

Richard M Krause 1
PMCID: PMC2136927  PMID: 13598814

Abstract

The lysis of cell walls of hemolytic streptococci by a phage-associated lysin has been described. A method is presented for preparing the lysin from Group C streptococcal phage lysates. Following lysis almost all of the cell wall carbohydrate is recovered in solution. This material has the serological reactivity, physical-chemical properties, and values for nitrogen, rhamnose, and glucosamine similar to those of the carbohydrate isolated from the cell walls by the Streptomyces albus enzyme. Group C carbohydrate isolated by either enzyme inactivates Group C bacteriophage. The protein liberated by the lysin from Group A Type 6 cell walls gives a type-specific precipitin reaction with homologous rabbit antiserum. Preliminary data are presented on the ammonium sulfate fractionation and the electrophoretic separation of a protein fraction with the serological reactivity of M protein.

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Selected References

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