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. 1959 Feb 28;109(3):311–324. doi: 10.1084/jem.109.3.311

A HISTOLOGIC STUDY OF THE AUTO-ALLERGIC TESTIS LESION IN THE GUINEA PIG

Byron H Waksman 1
PMCID: PMC2136948  PMID: 13620856

Abstract

The auto-allergic lesion in guinea pigs inoculated with homologous testis plus the Freund adjuvant was investigated histologically. The lesion was found to consist of disseminated foci of perivenous inflammation, lymphocytes and histiocytes predominating in the cellular infiltrate, with invasion of epididymal, rete, and seminiferous tubules and destruction of tubular contents. Guinea pigs up to 800 gm. showed a rapidly progressing diffuse hypo- or aspermatogenesis, which appeared to be secondary to the inflammatory disease. In these animals, the process resolved leaving an atrophic testis with few or no indications of the preceding inflammation and fibrotic scarring only in the rare instances in which actual necrosis of connective tissue elements had occurred. In 1200 gm. animals there was no general hypospermatogenesis and the late findings were limited to foci of aspermatogenesis. This disease then is an experimental auto-allergic orchitis followed by testicular atrophy without scarring. Its morphologic similarity to mumps orchitis and to sterility with "germinal cell aplasia" in man is commented on.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DELAUNAY A., VOISIN G. Sur des lésions testiculaires provoquées chez le cobaye et chez le rat par l'endotoxine typhique. C R Hebd Seances Acad Sci. 1952 Jan 2;234(1):158–160. [PubMed] [Google Scholar]
  2. FREUND J., LIPTON M. M., THOMPSON G. E. Aspermatogenesis in the guinea pig induced by testicular tissue and adjuvants. J Exp Med. 1953 May;97(5):711–726. doi: 10.1084/jem.97.5.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FREUND J., LIPTON M. M., THOMPSON G. E. Impairment of spermatogenesis in the rat after cutaneous injection of testicular suspension with complete adjuvants. Proc Soc Exp Biol Med. 1954 Nov;87(2):408–411. doi: 10.3181/00379727-87-21396. [DOI] [PubMed] [Google Scholar]
  4. FREUND J., THOMPSON G. E., LIPTON M. M. Aspermatogenesis, anaphylaxis, and cutaneous sensitization induced in the guinea pig by homologous testicular extract. J Exp Med. 1955 Jun 1;101(6):591–604. doi: 10.1084/jem.101.6.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farber S., Wolbach S. B. Intranuclear and Cytoplasmic Inclusions ("Protozoan-Like Bodies") in the Salivary Glands and Other Organs of Infants. Am J Pathol. 1932 Mar;8(2):123–136.3. [PMC free article] [PubMed] [Google Scholar]
  6. Gall E. A. The Histopathology of Acute Mumps Orchitis. Am J Pathol. 1947 Jul;23(4):637–651. [PMC free article] [PubMed] [Google Scholar]
  7. HURST E. W. Experimental demyelination in relation to human and animal disease. Am J Med. 1952 May;12(5):547–560. doi: 10.1016/0002-9343(52)90235-0. [DOI] [PubMed] [Google Scholar]
  8. LUMSDEN C. E., KABAT E. A., WOLF A., BEZER A. E. Studies on acute disseminated encephalomyelitis produced experimentally in Rhesus monkeys; complement-fixing antibodies. J Exp Med. 1950 Sep;92(3):253–270. doi: 10.1084/jem.92.3.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RAFFEL S. Bacterial hypersensitivity. J Allergy. 1956 May;27(3):199–212. doi: 10.1016/0021-8707(56)90050-8. [DOI] [PubMed] [Google Scholar]
  10. Stewart F. W. Observations on Focal Tuberculin Hypersensitiveness in an Infected Organ Previous to a General Sensitization. Am J Pathol. 1925 Sep;1(5):495–502.7. [PMC free article] [PubMed] [Google Scholar]
  11. VOISIN G., DELAUNAY A., BARBER M. Sur des lesions testiculaires provoquées chez le cobaye par iso- et autosensibilisation. Ann Inst Pasteur (Paris) 1951 Jul;81(1):48–63. [PubMed] [Google Scholar]
  12. VOISIN G., DELAUNAY A. Mise en évidence d'un état d'iso- ou d'auto-sensibilisation au moyen du choc anaphylactique. Ann Inst Pasteur (Paris) 1955 Nov;89(5):556–581. [PubMed] [Google Scholar]
  13. VOISIN G., DELAUNAY A. Sur les lésions testiculaires observées chez des animaux soumis a des injections de substances adjuvantes seules ou mélangées avec des extraits de tissus homologues. Ann Inst Pasteur (Paris) 1955 Sep;89(3):307–317. [PubMed] [Google Scholar]
  14. WAKSMAN B. H. Further study of skin reactions in rabbits with experimental allergic encephalomyelitis: reactions to tests with purified white matter fractions; use of a quantitative technique for evaluating sensitivity production of sensitization and disease by intradermal injection of antigen without adjuvants. J Infect Dis. 1956 Nov-Dec;99(3):258–269. doi: 10.1093/infdis/99.3.258. [DOI] [PubMed] [Google Scholar]
  15. WAKSMAN B. H., MORRISON L. R. Tuberculin type sensitivity to spinal cord antigen in rabbits with isoallergic encephalomyelitis. J Immunol. 1951 Apr;66(4):421–444. [PubMed] [Google Scholar]
  16. WAKSMAN B. H., PORTER H., LEES M. D., ADAMS R. D., FOLCH J. A study of the chemical nature of components of bovine white matter effective in producing allergic encephalomyelitis in the rabbit. J Exp Med. 1954 Nov 1;100(5):451–471. doi: 10.1084/jem.100.5.451. [DOI] [PMC free article] [PubMed] [Google Scholar]

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