Abstract
Hemolysis of chicken red blood cells by mumps virus is associated with the release of sphingomyelin from the stromal lipoprotein and the destruction of 65 per cent of the sphingomyelin of the red cell stroma. However, the virus had no effect on isolated phosphatides extracted from the erythrocytes. The hemolytic action of the virus and changes in sphingomyelin content of the erythrocytes fail to occur at a pH of 6.0. The viral hemolysis of human erythrocytes is not associated with similar alterations in their content of sphingomyelin. The absence of lecithin from sheep erythrocytes, which are also lysed by mumps virus, is additional evidence that a viral lecithinase is not associated with the hemolytic property of mumps virus. Mumps virus concentrated from the amniotic fluid of viral infected chick embryos contains about 7 per cent phosphatide, 60 per cent of which is sphingomyelin.
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Selected References
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- Chu L. W., Morgan H. R. STUDIES OF THE HEMOLYSIS OF RED BLOOD CELLS BY MUMPS VIRUS : I. THE DEVELOPMENT OF MUMPS VIRUS HEMOLYSIN AND ITS INACTIVATION BY CERTAIN PHYSICAL AND CHEMICAL AGENTS. J Exp Med. 1950 Mar 31;91(4):393–402. doi: 10.1084/jem.91.4.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FROMMHAGEN L. H., FREEMAN N. K., KNIGHT C. A. The lipid constituents of influenza virus, chick allantoic membrane and sedimentable allantoic protein. Virology. 1958 Feb;5(1):173–175. doi: 10.1016/0042-6822(58)90015-1. [DOI] [PubMed] [Google Scholar]
- GARDNER B. J., MORGAN H. R. Effect of certain enzyme inhibitors on hemolytic and hemagglutinating activity of mumps virus. Proc Soc Exp Biol Med. 1952 Jan;79(1):133–135. doi: 10.3181/00379727-79-19297. [DOI] [PubMed] [Google Scholar]
- HENLE G., DEINHARDT F. Propagation and primary isolation of mumps virus in tissue culture. Proc Soc Exp Biol Med. 1955 Aug;89(4):556–560. doi: 10.3181/00379727-89-21873. [DOI] [PubMed] [Google Scholar]
- HOYER B. H., BOLTON E. T., ORMSBEE R. A., LEBOUVIER G., RITTER D. B., LARSON C. L. Mammalian viruses and Rickettsiae; their purification and recovery by cellulose anion exchange columns has significant implications. Science. 1958 Apr 18;127(3303):859–863. doi: 10.1126/science.127.3303.859. [DOI] [PubMed] [Google Scholar]
- KORN E. D. Clearing factor, a heparin-activated lipoprotein lipase. II. Substrate specificity and activation of coconut oil. J Biol Chem. 1955 Jul;215(1):15–26. [PubMed] [Google Scholar]
- MANSON L. A., ROTHSTEIN E. L., RAKE G. W. Purification of poliovirus with fluorocarbon. Science. 1957 Mar 22;125(3247):546–547. doi: 10.1126/science.125.3247.546-a. [DOI] [PubMed] [Google Scholar]
- MARINETTI G. V., ERBLAND J., KOCHEN J. Quantitative chromatography of phosphatides. Fed Proc. 1957 Sep;16(3):837–844. [PubMed] [Google Scholar]
- MARINETTI G. V., WITTER R. F., STOTZ E. The incorporation in vivo of P32-labeled orthophosphate into individual phosphatides of rat tissues. J Biol Chem. 1957 May;226(1):475–483. [PubMed] [Google Scholar]
- MOBERLY M. L., MARINETTI G. V., WITTER R. F., MORGAN H. R. Studies of hemolysis of red blood cells by mumps virus. III. Alterations in lipoproteins of the red blood cell wall. J Exp Med. 1958 Jan 1;107(1):87–94. doi: 10.1084/jem.107.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MORGAN H. R., ENDERS J. F., WAGLEY P. F. A hemolysin associated with the mumps virus. J Exp Med. 1948 Nov;88(5):503–514. doi: 10.1084/jem.88.5.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MORGAN H. R. Factors interfering with the hemolytic property of mumps virus. Proc Soc Exp Biol Med. 1951 Jun;77(2):276–278. doi: 10.3181/00379727-77-18748. [DOI] [PubMed] [Google Scholar]
- POLLARD M., CONNOLLY J., FROMM S. The precipitating effect of methanol on viruses. Proc Soc Exp Biol Med. 1949 Jun;71(2):290–293. doi: 10.3181/00379727-71-17167. [DOI] [PubMed] [Google Scholar]
- TURNER J. C. Absence of lecithin from the stromata of the red cells of certain animals (ruminants), and its relation to venom hemolysis. J Exp Med. 1957 Mar 1;105(3):189–193. doi: 10.1084/jem.105.3.189. [DOI] [PMC free article] [PubMed] [Google Scholar]