Abstract
Ribonucleic acid extracted with phenol from Type 1 poliovirus, Coxsackie A-9, Coxsackie B-1, and ECHO 8 viruses infected non-primate cells and animals insusceptible to whole virus as such. Viral RNA was proved infectious for insusceptible cells in test systems of established cell lines, primary monolayer cultures, Maitland type cultures, and living animals inoculated intracerebrally. Cells of rabbit, swine, mouse, guinea pig, chicken, and hamster were infected. Each virus produced was identical with the virus donating RNA, in (a) neutralization by homotypic antiserum, (b) resistance to ribonuclease treatment, and (c) failure to be adsorbed or replicated by nonprimate cells, even of the strain producing the virus from RNA. Produced virus was adsorbed and replicated by susceptible primate cells as usual. Virus in RNA-infected cell cultures was produced in a single cycle unaccompanied by overt cytopathic effect on non-primate cells or disease of intracerebrally inoculated animals. By drastic elution of infective poliovirus associated with rabbit cells exposed to massive inocula of intact virus, intact poliovirus was shown to infect insusceptible non-primate cells to produce progeny indistinguishable from the parent virus population. Under these conditions, infection was accomplished by about 10 virus plaque-forming units per billion inoculated.
Full Text
The Full Text of this article is available as a PDF (857.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ALEXANDER H. E., KOCH G., MOUNTAIN I. M., VAN DAMME O. Infectivity of ribonucleic acid from poliovirus in human cell monolayers. J Exp Med. 1958 Oct 1;108(4):493–506. doi: 10.1084/jem.108.4.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COLTER J. S., BIRD H. H., MOYER A. W., BROWN R. A. Infectivity of ribonucleic acid isolated from virus-infected tissues. Virology. 1957 Dec;4(3):522–532. doi: 10.1016/0042-6822(57)90084-3. [DOI] [PubMed] [Google Scholar]
- Fraser D., Mahler H. R., Shug A. L., Thomas C. A. THE INFECTION OF SUB-CELLULAR ESCHERICHIA COLI, STRAIN B, WITH A DNA PREPARATION FROM T2 BACTERIOPHAGE. Proc Natl Acad Sci U S A. 1957 Nov 15;43(11):939–947. doi: 10.1073/pnas.43.11.939. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GAREN A., PUCK T. T. The first two steps of the invasion of host cells by bacterial viruses. II. J Exp Med. 1951 Sep;94(3):177–189. doi: 10.1084/jem.94.3.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOLLAND J. J., McLAREN L. C., SYVERTON J. T. Mammalian cell-virus relationship. III. Poliovirus production by non-primate cells exposed to poliovirus ribonucleic acid. Proc Soc Exp Biol Med. 1959 Apr;100(4):843–845. doi: 10.3181/00379727-100-24798. [DOI] [PubMed] [Google Scholar]
- HOLLAND J. J., McLAREN L. C. The mammalian cell-virus relationship. II. Adsorption, reception, and eclipse of poliovirus by HeLa cells. J Exp Med. 1959 May 1;109(5):487–504. doi: 10.1084/jem.109.5.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McLAREN L. C., HOLLAND J. J., SYVERTON J. T. The mammalian cell-virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non-primate origin. J Exp Med. 1959 May 1;109(5):475–485. doi: 10.1084/jem.109.5.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PRINCE A. M., GINSBERG H. S. Immunohistochemical studies on the interaction between Ehrlich ascites tumor cells and Newcastle disease virus. J Exp Med. 1957 Feb 1;105(2):177–188. doi: 10.1084/jem.105.2.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROIZMAN B., HOPKEN W., MAYER M. M. Immunochemical studies of poliovirus. II. Kinetics of the formation of infectious and noninfectious type 1 poliovirus in three cell strains of human derivation. J Immunol. 1958 May;80(5):386–395. [PubMed] [Google Scholar]
- SCHAFFER F. L., SCHWERDT C. E. Purification of poliomyelitis viruses propagated in tissue culture. Virology. 1956 Oct;2(5):665–678. doi: 10.1016/0042-6822(56)90046-0. [DOI] [PubMed] [Google Scholar]
- SCHERER W. F. Studies on the propagation in vitro of poliomyelitis viruses. VI. Effect on virus yield of cell population, virus inoculum and temperature of incubation. J Immunol. 1954 Nov;73(5):331–336. [PubMed] [Google Scholar]
- TAKAHASHI W. N., KARLER A., KNIGHT C. A. Electrochromatographic studies on infections nucleic acid from tobacco mosaic virus. Virology. 1958 Dec;6(3):637–648. doi: 10.1016/0042-6822(58)90112-0. [DOI] [PubMed] [Google Scholar]
- YOUNGNER J. S. Virus yield per cell in monkey kidney cultures inoculated with different strains of poliomyelitis virus. J Immunol. 1956 Jan;76(1):50–53. [PubMed] [Google Scholar]