Abstract
Serum and serum fractions from patients with rheumatoid arthritis have been subjected to anion exchange chromatography on diethylaminoethyl cellulose. The rheumatoid factor activity was clearly separated from the bulk of the gamma globulin by virtue of the anomalous behavior of macroglobulins on this column. Purification of rheumatoid factor by precipitation with fraction II, resolution in 4 M urea, and two successive fractionations on an anion exchanger yielded a highly active preparation consisting of approximately 95 per cent 19S gamma globulin with no detectable amount of 7S contaminant. Evidence was obtained for the existence of two factors by cation exchange chromatography (carboxymethyl cellulose) of rheumatoid serum and of macroglobulin previously separated by anion exchange chromatography. One factor (factor I) agglutinated sensitized sheep cells and latex particles and also precipitated with human fraction II. The second (factor II) agglutinated latex partides and precipitated with fraction II but did not agglutinate sensitized sheep cells. A substance with the properties of factor II was demonstrated in two of four normal sera by cation exchange chromatography. It was suggested that the agglutination of latex particles and precipitation with human fraction II, observed in the case of certain abnormal sera, may be due to elevated concentrations of factor II.
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Selected References
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- EDELMAN G. M., KUNKEL H. G., FRANKLIN E. C. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. doi: 10.1084/jem.108.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EPSTEIN W., JOHNSON A., RAGAN C. Observations on a precipitin reaction between serum of patients with rheumatoid arthritis and a preparation (Cohn fraction II) of human gamma globulin. Proc Soc Exp Biol Med. 1956 Feb;91(2):235–237. doi: 10.3181/00379727-91-22223. [DOI] [PubMed] [Google Scholar]
- FALLET G. H., LOSPALLUTO J., ZIFF M. Chromatographic and electrophoretic studies of the L.E. factor. Arthritis Rheum. 1958 Oct;1(5):419–434. doi: 10.1002/art.1780010506. [DOI] [PubMed] [Google Scholar]
- FRANKLIN E. C., KUNKEL H. G., MULLER-EBERHARD H. J. Two types of gamma-globulin differing in carbohydrate content. Proc Soc Exp Biol Med. 1956 Oct;93(1):146–150. doi: 10.3181/00379727-93-22690. [DOI] [PubMed] [Google Scholar]
- KUNKEL H. G., SIMON H. J., FUDENBERG H. Observations concerning positive serologic reactions for rheumatoid factor in certain patients with sarcoidosis and other hyperglobulinemic states. Arthritis Rheum. 1958 Aug;1(4):289–296. doi: 10.1002/art.1780010402. [DOI] [PubMed] [Google Scholar]
- LAMONT-HAVERS R. W. Nature of serum factors causing agglutination of sensitized sheep cells and group A hemolytic streptococci. Proc Soc Exp Biol Med. 1955 Jan;88(1):35–38. doi: 10.3181/00379727-88-21485. [DOI] [PubMed] [Google Scholar]
- MOKRASCH L. C. Analysis of hexose phosphates and sugar mixtures with the anthrone reagent. J Biol Chem. 1954 May;208(1):55–59. [PubMed] [Google Scholar]
- PLOTZ C. M., SINGER J. M. The latex fixation test. I. Application to the serologic diagnosis of rheumatoid arthritis. Am J Med. 1956 Dec;21(6):888–892. [PubMed] [Google Scholar]
- PREER J. R., Jr A quantitative study of a technique of double diffusion in agar. J Immunol. 1956 Jul;77(1):52–60. [PubMed] [Google Scholar]
- SVARTZ N., CARLSON L. A., SCHLOSSMANN K., EHRENBERG A. Isolation of the rheumatoid factor; preliminary report. Acta Med Scand. 1958 Mar 7;160(2):87–90. doi: 10.1111/j.0954-6820.1958.tb10330.x. [DOI] [PubMed] [Google Scholar]
- SVARTZ N., SCHLOSSMANN K. A serum precipitable hemagglutinating factor in rheumatoid arthritis. Acta Med Scand. 1954;149(2):83–89. [PubMed] [Google Scholar]
- ZIFF M., BROWN P., LOSPALLUTO J., BADIN J., MCEWEN C. Agglutination and inhibition by serum globulin in the sensitized sheep cell agglutination reaction in rheumatoid arthritis. Am J Med. 1956 Apr;20(4):500–509. doi: 10.1016/0002-9343(56)90134-6. [DOI] [PubMed] [Google Scholar]
- ZIFF M. The agglutination reaction in rheumatoid arthritis. J Chronic Dis. 1957 Jun;5(6):644–667. doi: 10.1016/0021-9681(57)90074-7. [DOI] [PubMed] [Google Scholar]