Abstract
Although induction of contact skin sensitivity by low molecular weight 2,4-dinitrobenzenes requires the formation in vivo of 2,4-dinitrophenyl-proteins, analogous protein conjugates prepared in vitro are unable to induce this hypersensitive state. Low molecular weight 2,4-dinitrobenzenes are concentrated by isolated lymph node cells, but a representative 2,4-dinitrophenyl-protein conjugate (2,4-dinitrophenyl-bovine serum albumin) was not taken up to a detectable extent by these cells. It is inferred that there exist large quantitative differences in the extent to which dinitrophenyl-proteins are localized within cells following the administration to an intact animal of (a) those simple dinitrobenzenes which are both concentrated by lymph node cells and have the capacity to form protein conjugates in vivo, and (b) 2,4-dinitrophenyl-protein conjugates prepared in vitro. It is suggested that this difference could account for the fact that a varietyof 2,4-dinitrophenyl-proteins prepared in vitro are unable to induce contact skin sensitivity.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHASE M. W. Experimental sensitization with particular reference to picryl chloride. Int Arch Allergy Appl Immunol. 1954;5(2):163–191. doi: 10.1159/000228095. [DOI] [PubMed] [Google Scholar]
- COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE CARVALHO C. A., POGELL B. M. Modified skatole method for microdetermination of fructose and inulin. Biochim Biophys Acta. 1957 Oct;26(1):206–206. doi: 10.1016/0006-3002(57)90075-6. [DOI] [PubMed] [Google Scholar]
- EAGLE H. Nutrition needs of mammalian cells in tissue culture. Science. 1955 Sep 16;122(3168):501–514. doi: 10.1126/science.122.3168.501. [DOI] [PubMed] [Google Scholar]
- EISEN H. N., BELMAM S. Studies of hypersensitivity to low molecular weight substances. II. Reactions of some allergenic substituted dinitrobenzenes with cysteine or cystine of skin proteins. J Exp Med. 1953 Dec;98(6):533–549. doi: 10.1084/jem.98.6.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EISEN H. N., CARSTEN M. E., BELMAN S. Studies of hypersensitivity to low molecular weight substances. III. The 2,4-dinitrophenyl group as a determinant in the preciptin reaction. J Immunol. 1954 Nov;73(5):296–308. [PubMed] [Google Scholar]
- EISEN H. N., ORRIS L., BELMAN S. Elicitation of delayed allergic skin reactions with haptens; the dependence of elicitation on hapten combination with protein. J Exp Med. 1952 May 1;95(5):473–487. doi: 10.1084/jem.95.5.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EISEN H. N., TABACHNICK M. Elicitation of allergic contact dermatitis in the guinea pig; the distribution of bound dinitrobenzene groups within the skin and quantitative determination of the extent of combination of 2,4-dinitro-chlorobenzene with epidermal protein in vivo. J Exp Med. 1958 Dec 1;108(6):773–796. doi: 10.1084/jem.108.6.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HELMREICH E., CORI C. F. Studies of tissue permeability. II. The distribution of pentoses between plasma and muscle. J Biol Chem. 1957 Feb;224(2):663–679. [PubMed] [Google Scholar]
- KABAT E. A. Heterogeneity in extent of the combining regions of human antidextran. J Immunol. 1956 Dec;77(6):377–385. [PubMed] [Google Scholar]
- KEILIN D., HARTREE E. F. Specificity of glucose oxidase (notatin). Biochem J. 1952 Jan;50(3):331–341. doi: 10.1042/bj0500331. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KERN M., EISEN H. N. The effect of antigenic stimulation on incorporation of phosphate and methionine into proteins of isolated lymph node cells. J Exp Med. 1959 Aug 1;110(2):207–219. doi: 10.1084/jem.110.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landsteiner K., Chase M. W. STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS : IV. ANAPHYLAXIS INDUCED BY PICRYL CHLORIDE AND 2:4 DINITROCHLOROBENZENE. J Exp Med. 1937 Aug 31;66(3):337–351. doi: 10.1084/jem.66.3.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landsteiner K., Chase M. W. STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS : IX. SKIN SENSITIZATION INDUCED BY INJECTION OF CONJUGATES. J Exp Med. 1941 Feb 28;73(3):431–438. doi: 10.1084/jem.73.3.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landsteiner K., Jacobs J. STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS. II. J Exp Med. 1936 Sep 30;64(4):625–639. doi: 10.1084/jem.64.4.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landsteiner K., Jacobs J. STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS. J Exp Med. 1935 Apr 30;61(5):643–656. doi: 10.1084/jem.61.5.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAYER R. L. The role of the carrier in the formation of complete antigens. J Allergy. 1957 May;28(3):191–205. doi: 10.1016/0021-8707(57)90124-7. [DOI] [PubMed] [Google Scholar]
- PAPPENHEIMER A. M., SALVIN S. B., UHR J. W. Delayed hypersensitivity. II. Induction of hypersensitivity in guinea pigs by means of antigen-antibody complexes. J Exp Med. 1957 Jan 1;105(1):11–24. doi: 10.1084/jem.105.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROE J. H., EPSTEIN J. H., GOLDSTEIN N. P. A photometric method for the determination of insulin in plasma and urine. J Biol Chem. 1949 Apr;178(2):839–845. [PubMed] [Google Scholar]
- Sanger F. The free amino groups of insulin. Biochem J. 1945;39(5):507–515. doi: 10.1042/bj0390507. [DOI] [PMC free article] [PubMed] [Google Scholar]