Abstract
Total virus particle counts, infectivity titrations and the ratios between particles and infective units have been determined for vaccinia virus infected tissues. Growth curves of vaccinia in the chorioallantoic membrane are characterized by relatively low ratios from 1 to 4 days after inoculation and a marked rise in the ratio at more prolonged intervals. Ratio determinations of vaccinia virus passages in the egg, rabbit skin, and guinea pig skin have been made to study the phenomenon of adaptation in different hosts. The embryonated egg chorioallantoic membrane shows no variation in the ratio of particles to infectious units during passage and it is concluded that this host is completely susceptible to vaccinia. During adaptive passages on the skin of rabbits and guinea pigs relatively large amounts of non-infective virus appear as indicated by a rise in the particle-infectivity ratios. The extent of ratio increase appears related to the general resistance of the host to the virus. Finally, treatment of crude tissue extracts with sonic vibration is described as an aid in dispersing the virus particles for quantitative particle counts.
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Selected References
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- CROCKER T. T. The number of elementary bodies per 50% lethal dose of meningopneumonitis virus as determined by electron microscopic counting. J Immunol. 1954 Jul;73(1):1–7. [PubMed] [Google Scholar]
- DUMBELL K. R., DOWNIE A. W., VALENTINE R. C. The ratio of the number of virus particles to infective titer of cowpox and vaccinia virus suspensions. Virology. 1957 Dec;4(3):467–482. doi: 10.1016/0042-6822(57)90080-6. [DOI] [PubMed] [Google Scholar]
- ECKERT E. A., SHARP D. G., BEARD D., GREEN I., BEARD J. W. Neutralization and precipitation of the virus of avian erythromyeloblastosis with serum of hyperimmunized chickens. Proc Soc Exp Biol Med. 1955 Feb;88(2):181–187. doi: 10.3181/00379727-88-21530. [DOI] [PubMed] [Google Scholar]
- LURIA S. E., WILLIAMS R. C., BACKUS R. C. Electron micrographic counts of bacteriophage particles. J Bacteriol. 1951 Feb;61(2):179–188. doi: 10.1128/jb.61.2.179-188.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NADEJE T., TAMM I., OVERMAN J. R. A new technique for dropping the chorioallantoic membrane in embryonated chicken eggs. J Lab Clin Med. 1955 Oct;46(4):648–650. [PubMed] [Google Scholar]
- OVERMAN J. R., TAMM I. Equivalence between vaccinia particles counted by electron microscopy and infectious units of the virus. Proc Soc Exp Biol Med. 1956 Aug-Sep;92(4):806–810. doi: 10.3181/00379727-92-22621. [DOI] [PubMed] [Google Scholar]
- OVERMAN J. R., TAMM I. Quantitative titration of vaccinia virus on the chorioallantoic membrane. J Immunol. 1956 Mar;76(3):228–236. [PubMed] [Google Scholar]
- Rivers T. M., Smadel J. E., Chambers L. A. EFFECT OF INTENSE SONIC VIBRATIONS ON ELEMENTARY BODIES OF VACCINIA. J Exp Med. 1937 Apr 30;65(5):677–685. doi: 10.1084/jem.65.5.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHARP D. G., OVERMAN J. R. Enumeration of vaccinia virus particles in crude extracts of infected tissues by electron microscopy. Proc Soc Exp Biol Med. 1958 Nov;99(2):409–413. doi: 10.3181/00379727-99-24366. [DOI] [PubMed] [Google Scholar]
- Smadel J. E., Rivers T. M., Pickels E. G. ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA. J Exp Med. 1939 Sep 30;70(4):379–385. doi: 10.1084/jem.70.4.379. [DOI] [PMC free article] [PubMed] [Google Scholar]