Abstract
A protein of unusual characteristics has been identified in normal rat sera by electrophoretic separation at pH 8.6, followed by precipitation of each fraction with a cationic detergent. This protein, which is closely identified with albumin after electrophoresis at pH 8.6, precipitates with cationic detergent at a low pH in contrast to albumin which precipitates with detergent only at a high pH. This protein has characteristics of a globulin and is designated as a "fast alpha fraction." This fraction and albumin are present in about equal amounts in sera of normal rats. A separation of the fast alpha fraction can be made by electrophoresis in an acetate buffer of pH 4.25 and by precipitation according to a modified Cohn, cold-alcohol technique. A protein of similar characteristics has not been found in either human or rabbit sera studied by the same method of electrophoresis and subsequent sub-fractionation with cationic detergent.
Full Text
The Full Text of this article is available as a PDF (637.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DE LAMIRANDE G., CANTERO A. Electrophoretic analysis of plasma protein constituents in rats bearing regenerating and pre-neoplastic livers. Cancer Res. 1952 May;12(5):330–333. [PubMed] [Google Scholar]
- HOCH-LIGETI C., HOCH H., GOODALL K. Investigations on the serum proteins in rats during the development of hepatic tumours due to feeding p-dimethylaminoazobenzene. Br J Cancer. 1949 Mar;3(1):140–147. doi: 10.1038/bjc.1949.16. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JACOX R. F., FELDMAHN A. A study of plasma protein alterations in rabbits infected with type I pneumococci. J Exp Med. 1956 May 1;103(5):633–652. doi: 10.1084/jem.103.5.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JACOX R. F. Quantitative fractionation of component proteins of human serum with cationic detergents. J Clin Invest. 1953 Jul;32(7):661–673. doi: 10.1172/JCI102779. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUNKEL H. G. Zone electrophoresis. Methods Biochem Anal. 1954;1:141–170. doi: 10.1002/9780470110171.ch6. [DOI] [PubMed] [Google Scholar]
- SCHEIFFARTH F., BERG G. Serumeiweissbestimmungen bei Ratten und Kaninchen mit der Papierelektrophorese. I. Die Normalwerte bei Ratten und Kaninchen. Z Gesamte Exp Med. 1952;119(5):550–558. [PubMed] [Google Scholar]
- WESTPHAL U., PRIEST S. G., STETS J. F., SELDEN G. L. Influence of whole body X-irradiation, cold exposure and experimental acidosis on protein composition and azorubin-binding capacity of rat serum. Am J Physiol. 1953 Dec;175(3):424–428. doi: 10.1152/ajplegacy.1953.175.3.424. [DOI] [PubMed] [Google Scholar]