Abstract
The interaction of sensitized sheep crythrocytes (EA) with guinea pig complement inactivated by treatment with hydrazine or ether has been studied. In both cases EA is converted into EAC'1. The hemolytic reactivity of this complex with respect to a complement reagent that contains C'4, C'2, and C'3 (R1) decays rapidly in the presence of hydrazine-treated complement (R4) or decomplemented guinea pig serum, but not in the presence of ether-treated guinea pig serum or of saline. The rate of decay of EAC'1 is dependent on the temperature, the reaction volume, and the amount of the inactivating reagent. The capacity of R4 and of decomplemented serum to inactivate EAC'1 can be markedly reduced by treatment with ether. The essential conditions for obtaining an optimally reactive EAC'1 consist in exposing EA to ether-treated guinea pig serum in the cold. This procedure yields a highly reactive EAC'1, even when the cells are sensitized with suboptimal amounts of amboceptor.
Full Text
The Full Text of this article is available as a PDF (702.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- KLEIN P. G., BURKHOLDER P. M. Studies on the antigenic properties of complement. II. Analysis of specific agglutinins against certain components of guinea pig complement fixed on sensitized sheep erythrocytes. J Exp Med. 1960 Jan 1;111:107–117. doi: 10.1084/jem.111.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEPOW I. H., PILLEMER L. Studies on the mechanism of inactivation of human complement by plasmin and by antigen-antibody aggregates. II. Demonstration of two distinct reaction stages in complement fixation. J Immunol. 1955 Jul;75(1):63–70. [PubMed] [Google Scholar]
- MAYER M. M., CROFT C. C., GRAY M. M. Kinetic studies on immune hemolysis; a method. J Exp Med. 1948 Oct 1;88(4):427–444. doi: 10.1084/jem.88.4.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAYER M. M., LEVINE L., RAPP H. J., MARUCCI A. A. Kinetic studies on immune hemolysis. VII. Decay of EAC'1, 4, 2, fixation of C'3, and other factors influencing the hemolytic action of complement. J Immunol. 1954 Dec;73(6):443–454. [PubMed] [Google Scholar]
- Pillemer L., Seifter S., Chu F., Ecker E. E. FUNCTION OF COMPONENTS OF COMPLEMENT IN IMMUNE HEMOLYSIS. J Exp Med. 1942 Jul 1;76(1):93–101. doi: 10.1084/jem.76.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]