Abstract
Skin sensitizing human antibodies were conjugated with various fluorescent dyes without significant loss in their ability to combine with specific antigen in vitro. A biopsy of the skin site challenged with egg albumin in a patient sensitive to this antigen could be stained specifically by the fluorescent reagins. The epithelial cells of the epidermis, sweat glands, hair follicles, and sebaceous glands in such a challenged site showed specific staining. In addition to the epithelial cells, the most intense staining was in macrophages and in pericapillary cells. The endothelium of the small blood vessels stained less intensely. Fibrous tissue bundles were specifically stained. The immunologic staining with the conjugated reagins was similar to but more intense than that obtained with conjugated rabbit anti-egg albumin globulins.
Full Text
The Full Text of this article is available as a PDF (749.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BUCKLEY S. M., WHITNEY E., RAPP F. Identification by fluorescent antibody of developmental forms of psittacosis virus in tissue culture. Proc Soc Exp Biol Med. 1955 Oct;90(1):226–230. doi: 10.3181/00379727-90-21990. [DOI] [PubMed] [Google Scholar]
- CHADWICK C. S., McENTEGART M. G., NAIRN R. C. Fluorescent protein tracers; a trial of new fluorochromes and the development of an alternative to fluorescein. Immunology. 1958 Oct;1(4):315–327. [PMC free article] [PubMed] [Google Scholar]
- COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DINEEN J. K., ADA G. L. Rapid extraction with ethyl acetate of free fluorescein derivatives from fluorescein isocyanate-globulin conjugates. Nature. 1957 Dec 7;180(4597):1284–1284. doi: 10.1038/1801284a0. [DOI] [PubMed] [Google Scholar]
- GITIL D., LANDING B. H., WHIPPLE A. The localization of homolgous plasma proteins in the tissues of young human beings as demonstrated with fluorescent antibodies. J Exp Med. 1953 Feb 1;97(2):163–176. doi: 10.1084/jem.97.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KING E. S., HUGHES P. E., LOUIS C. J. The species non-specificity of globulins in the globulin-fluorescein staining of tissues. Br J Cancer. 1958 Mar;12(1):5–13. doi: 10.1038/bjc.1958.2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARSHALL J. M., Jr Localization of adrenocorticotropic hormone by histochemical and immunochemical methods. J Exp Med. 1951 Jul 1;94(1):21–30. doi: 10.1084/jem.94.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RIGGS J. L., SEIWALD R. J., BURCKHALTER J. H., DOWNS C. M., METCALF T. G. Isothiocyanate compounds as fluorescent labeling agents for immune serum. Am J Pathol. 1958 Nov-Dec;34(6):1081–1097. [PMC free article] [PubMed] [Google Scholar]
- WELLER T. H., COONS A. H. Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro. Proc Soc Exp Biol Med. 1954 Aug-Sep;86(4):789–794. doi: 10.3181/00379727-86-21235. [DOI] [PubMed] [Google Scholar]