Abstract
An Hfr strain of E. coli K-12 has been shown to mate at low frequency with a number of strains of S. typhosa. The hybrids, selected as lactose positives, retained all other antigenic and biochemical manifestations of the S. typhosa parent. A Lac+ hybrid, S. typhosa strain 643L+ was remated with the E. coli Hfr and plated on minimal media containing L-arabinose, D-xylose, L-rhamnose, or L-fucose as the sole carbon sources. Hybrids of the remated strain appeared at high frequency on the plates containing L-arabinose, and could be detected at lower frequencies on plates containing D-xylose, L-rhamnose, and L-fucose. Those selected for D-xylose or L-rhamnose utilization possessed the attributes of segregating diploid heterozygotes being highly unstable and continually segregating a cultural form typical of the S. typhosa parent. The unstable type exhibited most of the biochemical characteristics of the E. coli parent including the ability to produce indol, and also reacted with antiserum to both the E. coli and S. typhosa parent strains, owing to the acquisition of a thermolabile antigen from the E. coli parent. The parent and hybrid strains were examined in detail for changes in patterns of phage susceptibility and virulence. Acquisition of susceptibility to a number of the T phages, a characteristic of the E. coli parent, was observed in one of the hybrid types. A decrease in virulence of the diploid hybrid form of S. typhosa in the mouse virulence test was found.
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Selected References
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- BARON L. S., FORMAL S. B., SPILMAN W. Vi phage-host interaction in Salmonella typhosa. J Bacteriol. 1955 Feb;69(2):177–183. doi: 10.1128/jb.69.2.177-183.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BARON L. S., SPILMAN W. M., CAREY W. F. Hybridization of Salmonella species by mating with Escherichia coli. Science. 1959 Sep 4;130(3375):566–567. doi: 10.1126/science.130.3375.566. [DOI] [PubMed] [Google Scholar]
- BRINTON C. C., Jr Non-flagellar appendages of bacteria. Nature. 1959 Mar 21;183(4664):782–786. doi: 10.1038/183782a0. [DOI] [PubMed] [Google Scholar]
- Baron L. S., Carey W. F., Spilman W. M. GENETIC RECOMBINATION BETWEEN ESCHERICHIA COLI AND SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1959 Jul;45(7):976–984. doi: 10.1073/pnas.45.7.976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CAVALLI L. L., LEDERBERG J., LEDERBERG E. M. An infective factor controlling sex compatibility in Bacterium coli. J Gen Microbiol. 1953 Feb;8(1):89–103. doi: 10.1099/00221287-8-1-89. [DOI] [PubMed] [Google Scholar]
- FURNESS G. The transfer of motility and tyrosine requirement to Escherichia coli strain B by recombination with E. coli strain K12. J Gen Microbiol. 1958 Jun;18(3):782–786. doi: 10.1099/00221287-18-3-782. [DOI] [PubMed] [Google Scholar]
- LEDERBERG J., EDWARDS P. R. Sero-typic recombination in Salmonella. J Immunol. 1953 Oct;71(4):232–240. [PubMed] [Google Scholar]
- LEDERBERG J. Streptomycin resistance; a genetically recessive mutation. J Bacteriol. 1951 May;61(5):549–550. doi: 10.1128/jb.61.5.549-550.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LURIA S. E., BURROUS J. W. Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957 Oct;74(4):461–476. doi: 10.1128/jb.74.4.461-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tatum E. L., Lederberg J. Gene Recombination in the Bacterium Escherichia coli. J Bacteriol. 1947 Jun;53(6):673–684. doi: 10.1128/jb.53.6.673-684.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WOLLMAN E. L., JACOB F., HAYES W. Conjugation and genetic recombination in Escherichia coli K-12. Cold Spring Harb Symp Quant Biol. 1956;21:141–162. doi: 10.1101/sqb.1956.021.01.012. [DOI] [PubMed] [Google Scholar]