Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1960 Aug 1;112(2):403–417. doi: 10.1084/jem.112.2.403

IN VITRO STUDIES ON THE INTERACTION BETWEEN MOUSE PERITONEAL MACROPHAGES AND STRAINS OF SALMONELLA AND ESCHERICHIA COLI

Charles Jenkin 1, Baruj Benacerraf 1
PMCID: PMC2137224  PMID: 13789820

Abstract

Virulent strains of Salmonella opsonized with normal mouse plasma are never phagocytosed as well as avirulent strains. The virulent strains of Salmonella phagocytosed after opsonization with normal mouse plasma are able to multiply within normal mouse peritoneal macrophages, whereas under similar experimental conditions the avirulent strains are killed. When virulent strains of Salmonella are opsonized with specific antiserum or plasma from BCG-infected mice, they are treated by normal mouse macrophages as if they were avirulent. Virulent bacteria opsonized with BCG plasma are phagocytosed and killed better by peritoneal macrophages from BCG-infected mice, than peritoneal macrophages from normal mice.

Full Text

The Full Text of this article is available as a PDF (759.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENACERRAF B., SEBESTYEN M. M., SCHLOSSMAN S. A quantitative study of the kinetics of blood clearance of P32-labelled Escherichia coli and Staphylococci by the reticuloendothelial system. J Exp Med. 1959 Jul 1;110(1):27–48. doi: 10.1084/jem.110.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DUBOS R. J., SCHAEDLER R. W. Effects of cellular constituents of mycobacteria on the resistance of mice to heterologous infections I. Protective effects. J Exp Med. 1957 Nov 1;106(5):703–717. doi: 10.1084/jem.106.5.703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. ELBERG S. S., FONG J., SCHNEIDER P. Studies on tubercle bacillus-monocyte relationship. I. Quantitative analysis of effect of serum of animals vaccinated with BCG upon bacterium-monocyte system. J Exp Med. 1956 Oct 1;104(4):455–465. doi: 10.1084/jem.104.4.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FURNESS G. Interaction between Salmonella typhimurium and phagocytic cells in cell culture. J Infect Dis. 1958 Nov-Dec;103(3):272–277. doi: 10.1093/infdis/103.3.272. [DOI] [PubMed] [Google Scholar]
  5. GELZER J., SUTER E. The effect of antibody on intracellular parasitism of Salmonella typhimurium in mononuclear phagocytes in vitro: prolonged survival of infected monocytes in presence of antibody. J Exp Med. 1959 Nov 1;110:715–730. doi: 10.1084/jem.110.5.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HOWARD J. G., BIOZZI G., HALPERN B. N., STIFFEL C., MOUTON D. The effect of Mycobacterium tuberculosis (BCG) infection on the resistance of mice to bacterial endotoxin and Salmonella enteritidis infection. Br J Exp Pathol. 1959 Jun;40(3):281–290. [PMC free article] [PubMed] [Google Scholar]
  7. HOWARD J. G., ROWLEY D., WARDLAW A. C. Investigations on the mechanism of stimulation of non-specific immunity by bacterial lipopolysaccharides. Immunology. 1958 Jul;1(3):181–203. [PMC free article] [PubMed] [Google Scholar]
  8. Lurie M. B., Collaboration of Peter Zappasodi STUDIES ON THE MECHANISM OF IMMUNITY IN TUBERCULOSIS : THE FATE OF TUBERCLE BACILLI INGESTED BY MONONUCLEAR PHAGOCYTES DERIVED FROM NORMAL AND IMMUNIZED ANIMALS. J Exp Med. 1942 Mar 1;75(3):247–268. doi: 10.1084/jem.75.3.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MACKANESS G. B., SMITH N., WELLS A. Q. The growth of intracellular tubercle bacilli in relation to their virulence. Am Rev Tuberc. 1954 Apr;69(4):479–494. doi: 10.1164/art.1954.69.4.479. [DOI] [PubMed] [Google Scholar]
  10. MACKANESS G. B. The growth of tubercle bacilli in monocytes from normal and vaccinated rabbits. Am Rev Tuberc. 1954 Apr;69(4):495–504. doi: 10.1164/art.1954.69.4.495. [DOI] [PubMed] [Google Scholar]
  11. MARCUS S., ESPLIN D. W., DONALDSON D. M. Lack of bactericidal effect of mouse serum on a number of common microorganisms. Science. 1954 Jun 18;119(3103):877–877. doi: 10.1126/science.119.3103.877. [DOI] [PubMed] [Google Scholar]
  12. MORGAN J. F., MORTON H. J., PARKER R. C. Nutrition of animal cells in tissue culture; initial studies on a synthetic medium. Proc Soc Exp Biol Med. 1950 Jan;73(1):1–8. doi: 10.3181/00379727-73-17557. [DOI] [PubMed] [Google Scholar]
  13. Pike R. M., Mackenzie G. M. Virulence of Salmonella typhimurium: I. Analysis of Experimental Infection in Mice with Strains of High and Low Virulence. J Bacteriol. 1940 Aug;40(2):171–195. doi: 10.1128/jb.40.2.171-195.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Porter K. R., Claude A., Fullam E. F. A STUDY OF TISSUE CULTURE CELLS BY ELECTRON MICROSCOPY : METHODS AND PRELIMINARY OBSERVATIONS. J Exp Med. 1945 Mar 1;81(3):233–246. doi: 10.1084/jem.81.3.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ROWLEY D. Rapidly induced changes in the level of non-specific immunity in laboratory animals. Br J Exp Pathol. 1956 Jun;37(3):223–234. [PMC free article] [PubMed] [Google Scholar]
  16. SCHNEIDER H. A., ZINDER N. D. Nutrition of the host and natural resistance to infection. V. An improved assay employing genetic markers in the double strain inoculation test. J Exp Med. 1956 Feb 1;103(2):207–223. doi: 10.1084/jem.103.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SHEPARD C. C. Phagocytosis by HeLa cells and their susceptibility to infection by human tubercle bacilli. Proc Soc Exp Biol Med. 1955 Nov;90(2):392–396. doi: 10.3181/00379727-90-22043. [DOI] [PubMed] [Google Scholar]
  18. SUTER E. Multiplication of tubercle bacilli within mononuclear phagocytes in tissue cultures derived from normal animals and animals vaccinated with BCG. J Exp Med. 1953 Feb 1;97(2):235–245. doi: 10.1084/jem.97.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WHITBY J. L., ROWLEY D. The role of macrophages in the elimination of bacteria from the mouse peritoneum. Br J Exp Pathol. 1959 Aug;40:358–370. [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES