Abstract
Polysaccharides extracted from cell walls of 4 serological types of Group D streptococci were serologically type-specific. Acid hydrolysates of all 4 contained hexosamine (glucosamine), rhamnose and glucose. A polysaccharide extracted from 2 serological types by either alkaline treatment or mechanical disruption was serologically group-specific. Acid hydrolysates contained glucose and no other single component in comparable amount. The group-specific polysaccharide, which may be of a dextran-like nature, is probably situated wholly or in part at the surface of the streptococcus.
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- ABRAMS A. O-acetyl groups in the cell wall of Streptococcus faecalis. J Biol Chem. 1958 Feb;230(2):949–959. [PubMed] [Google Scholar]
- BAUMGARTEN W., JOHNSON R. F., FINGER R. F., PAGENKEMPER F. E. The isolation and purification of pancreatic deoxyribonuclease. Arch Biochem Biophys. 1958 Sep;77(1):206–215. doi: 10.1016/0003-9861(58)90056-0. [DOI] [PubMed] [Google Scholar]
- CUMMINS C. S., HARRIS H. The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character. J Gen Microbiol. 1956 Jul;14(3):583–600. doi: 10.1099/00221287-14-3-583. [DOI] [PubMed] [Google Scholar]
- ELLIOTT S. D. Group and type-specific polysaccharides of group D Streptococci. Nature. 1959 Oct 24;184(Suppl 17):1342–1342. doi: 10.1038/1841342a0. [DOI] [PubMed] [Google Scholar]
- ELLIOTT S. D. The crystallization and serological differentiation of a streptococcal proteinase and its precursor. J Exp Med. 1950 Sep;92(3):201–218. doi: 10.1084/jem.92.3.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elson L. A., Morgan W. T. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. doi: 10.1042/bj0271824. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keilin D., Hartree E. F. Properties of glucose oxidase (notatin): Addendum. Sedimentation and diffusion of glucose oxidase (notatin). Biochem J. 1948;42(2):221–229. [PMC free article] [PubMed] [Google Scholar]
- Lancefield R. C. A SEROLOGICAL DIFFERENTIATION OF HUMAN AND OTHER GROUPS OF HEMOLYTIC STREPTOCOCCI. J Exp Med. 1933 Mar 31;57(4):571–595. doi: 10.1084/jem.57.4.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MCCARTY M. The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus. II. Nature of the cellular substrate attacked by the lytic enzymes. J Exp Med. 1952 Dec;96(6):569–580. doi: 10.1084/jem.96.6.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCARTY M., LANCEFIELD R. C. Variation in the group-specific carbohydrate of group A streptococci. I. Immunochemical studies on the carbohydrates of variant strains. J Exp Med. 1955 Jul 1;102(1):11–28. doi: 10.1084/jem.102.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PETERSON R. G., HARTSELL S. E. The lysozyme spectrum of the gram-negative bacteria. J Infect Dis. 1955 Jan-Feb;96(1):75–81. doi: 10.1093/infdis/96.1.75. [DOI] [PubMed] [Google Scholar]
- Partridge S. M. Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall. Biochem J. 1948;42(2):238–250. doi: 10.1042/bj0420238. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALTON M. R. J., HORNE R. W. Studies of the bacterial cell wall. II. Methods of preparation and some properties of cell walls. Biochim Biophys Acta. 1951 Jul;7(2):177–197. doi: 10.1016/0006-3002(51)90017-0. [DOI] [PubMed] [Google Scholar]
- SALTON M. R. Studies of the bacterial cell wall. IV. The composition of the cell walls of some Gram-positive and Gram-negative bacteria. Biochim Biophys Acta. 1953 Apr;10(4):512–523. doi: 10.1016/0006-3002(53)90296-0. [DOI] [PubMed] [Google Scholar]
- SCHMIDT W. C. Group A streptococcus polysaccharide: studies on its preparation, chemical composition, and cellular localization after intravenous injection into mice. J Exp Med. 1952 Jan;95(1):105–118. doi: 10.1084/jem.95.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swift H. F., Wilson A. T., Lancefield R. C. TYPING GROUP A HEMOLYTIC STREPTOCOCCI BY M PRECIPITIN REACTIONS IN CAPILLARY PIPETTES. J Exp Med. 1943 Aug 1;78(2):127–133. doi: 10.1084/jem.78.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]