Abstract
A study has been made of the immunospecific, apple-green staining patterns imparted by fluorescent antibody to Rous virus in frozen sections of wing tumors produced in young chickens by various initiating doses of virus. Variations in the cellular site (cytoplasm, cell membrane, and nucleus), the distribution (particulate or homogeneous), and the quantity (little or much) of Rous viral antigens were seen. The per cent of sarcoma cells containing viral antigens was related to the tumor initiating dose of virus and to the infectivity titer of virus extracted from the wing tumor. With certain assumptions it was estimated that a quantity of virus approaching one tumor-producing dose (1 TD50) per tissue section was detectable with fluorescent antibody. Sarcoma cells containing viral antigens were identified as early as 4 days after inoculation of Rous virus. At this time viral antigens were also localized in the skeletal muscle of the inoculated wing in a characteristic focal pattern of deposition near or on the sarcolemma. This association between viral antigens and skeletal muscle fibers was observed also at 3 days after virus inoculation, when sarcoma cells were not yet demonstrable in the tissue sections. Viral antigens were detected in sarcoma cells in lung tumors of young chickens and in tumors of the chorioallantoic membrane of chick embryos.
Full Text
The Full Text of this article is available as a PDF (975.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BRYAN W. R., CALNAN D., MOLONEY J. B. Biological studies on the Rous sarcoma virus. III. The recovery of virus from experimental tumors in relation to initiating dose. J Natl Cancer Inst. 1955 Aug;16(1):317–335. [PubMed] [Google Scholar]
- DEUTSCH H. F. II. Separation of antibody-active proteins from various animal sera by ethanol fractionation techniques. Methods Med Res. 1952;5:284–300. [PubMed] [Google Scholar]
- EPSTEIN M. A. The identification of the Rous virus; a morphological and biological study. Br J Cancer. 1956 Mar;10(1):33–48. doi: 10.1038/bjc.1956.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAGUENAU F., DALTON A. J., MOLONEY J. B. A preliminary report of electron microscopic and bioassay studies on the Rous sarcoma I virus. J Natl Cancer Inst. 1958 Mar;20(3):633–649. [PubMed] [Google Scholar]
- MALMGREN R. A., FINK M. A., MILLS W. Demonstration of the intracellular location of Rous sarcoma virus antigen by fluorescein-labeled antiserums. J Natl Cancer Inst. 1960 Apr;24:995–1001. [PubMed] [Google Scholar]
- MUNROE J. S., SOUTHAM C. M. Tissue distribution of the Rous sarcoma virus during the incubation period. Cancer Res. 1959 Apr;19(3 Pt 1):303–306. [PubMed] [Google Scholar]
- Mellors R. C., Heimer R., Corcos J., Korngold L. CELLULAR ORIGIN OF RHEUMATOID FACTOR. J Exp Med. 1959 Nov 30;110(6):875–886. doi: 10.1084/jem.110.6.875. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RIGGS J. L., SEIWALD R. J., BURCKHALTER J. H., DOWNS C. M., METCALF T. G. Isothiocyanate compounds as fluorescent labeling agents for immune serum. Am J Pathol. 1958 Nov-Dec;34(6):1081–1097. [PMC free article] [PubMed] [Google Scholar]
- RUBIN H. The production of virus by Rous sarcoma cells. Ann N Y Acad Sci. 1957 Oct 21;68(2):459–472. doi: 10.1111/j.1749-6632.1957.tb56100.x. [DOI] [PubMed] [Google Scholar]