Abstract
The Rostock strain of fowl plague, the swine, A, A', and Asian strains of influenza A as well as their hemagglutinin and internal s antigen subunits obtained after ether splitting, were found to be morphologically indistinguishable when examined simultaneously. Hemagglutinin fractions reacted in a highly strain specific manner when tested by hemagglutination inhibition or by complement fixation using sera obtained after infection. With the same sera internal s antigen fractions were shown to be serologically distinguishable by complement fixation. This observation may stimulate interest in the feasibility of employing immunologic techniques for the study of nucleoproteins. The significance of the findings reported is discussed.
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- ANDREWES C. H., WORTHINGTON G. Some new or little-known respiratory viruses. Bull World Health Organ. 1959;20(2-3):435–443. [PMC free article] [PubMed] [Google Scholar]
- BERLIN B. S., McKINNEY R. W. A simple device for making emulsified vaccines. J Lab Clin Med. 1958 Oct;52(4):657–658. [PubMed] [Google Scholar]
- FABIYI A., LIEF F. S., HENLE W. Antigenic analysis of influenza viruses by complement fixation. II. The production of antisera to strain-specific V antigens in guinea pigs. J Immunol. 1958 Dec;81(6):467–477. [PubMed] [Google Scholar]
- FRISCH-NIGGEMEYER W., HOYLE L. The nucleic acid and carbohydrate content of influenza virus A and of virus fractions produced by ether disintegration. J Hyg (Lond) 1956 Jun;54(2):201–212. doi: 10.1017/s0022172400044466. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRIST N. R. The serological diagnosis of influenza in infancy. Scott Med J. 1957 Jun;2(6):249–254. doi: 10.1177/003693305700200603. [DOI] [PubMed] [Google Scholar]
- HENLE W., LIEF F. S., PAUCKER K. Studies on the soluble antigen of influenza virus. IV. Fractionation of elementary bodies labeled with radioactive phosphorus. Virology. 1956 Dec;2(6):798–810. doi: 10.1016/0042-6822(56)90059-9. [DOI] [PubMed] [Google Scholar]
- HENLE W., LIEF F. S. Studies on the soluble antigen of influenza virus. II. A comparison of the effects of sonic vibration and ether treatment of elementary bodies. Virology. 1956 Dec;2(6):772–781. doi: 10.1016/0042-6822(56)90057-5. [DOI] [PubMed] [Google Scholar]
- HENLE W., LIEF F. S. Studies on the soluble antigen of influenza virus. III. The decreased incorporation of S antigen into elementary bodies of increasing incompleteness. Virology. 1956 Dec;2(6):782–797. doi: 10.1016/0042-6822(56)90058-7. [DOI] [PubMed] [Google Scholar]
- HOYLE L., JOLLES B., MITCHELL R. G. The incorporation of radioactive phosphorus in the influenza virus and its distribution in serologically active virus fractions. J Hyg (Lond) 1954 Mar;52(1):119–127. doi: 10.1017/s0022172400027315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOYLE L., REED R., ASTBURY W. T. Electron microscope studies of the structure of the influenza virus. Nature. 1953 Feb 7;171(4345):256–257. doi: 10.1038/171256b0. [DOI] [PubMed] [Google Scholar]
- HOYLE L. Structure of the influenza virus; the relation between biological activity and chemical structure of virus fractions. J Hyg (Lond) 1952 Jun;50(2):229–245. doi: 10.1017/s0022172400019562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LIEF F. S., FABIYI A., HENLE W. Antigenic analyses of influenza viruses by complement fixation. I. The production of antibodies to the soluble antigen in guinea pigs. J Immunol. 1958 Jan;80(1):53–65. [PubMed] [Google Scholar]
- Lennette E. H., Horsfall F. L. STUDIES ON INFLUENZA VIRUS : THE COMPLEMENT-FIXING ANTIGEN OF INFLUENZA A AND SWINE INFLUENZA VIRUSES. J Exp Med. 1941 Apr 30;73(5):581–599. doi: 10.1084/jem.73.5.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wiener M., Henle W., Henle G. STUDIES ON THE COMPLEMENT FIXATION ANTIGENS OF INFLUENZA VIRUSES TYPES A AND B. J Exp Med. 1946 Mar 31;83(4):259–279. [PMC free article] [PubMed] [Google Scholar]