Abstract
Studies on the fate of type 1 polioviruses in two common species of flies were carried out. The amount of virus in carcasses and excreta at different times was determined by the plaque assay method. Flies and their excreta remained infective for 11 days when kept at room temperature or when incubated at 36°C. for 2 hours a day. Flies remained infective for 3 months when kept in hibernation. A relative increase in titer was found to occur between 9 and 18 hours after feeding if the flies were incubated at 36°C. for 5 to 15 hours a day. The peak occurred later, at 40 to 52 hours after feeding if less incubation was used. Titers in excreta were parallel to titers in carcasses. A twofold increase in titer over the initial feeding was observed on 3 occasions with type 1 Mahoney but not with the LSc strain of virus.
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Selected References
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- HSIUNG G. D., MELNICK J. L. Morphologic characteristics of plaques produced on monkey kidney monolayer cultures by enteric viruses (poliomyelitis, Coxsackie, and echo groups. J Immunol. 1957 Feb;78(2):128–135. [PubMed] [Google Scholar]
- MELNICK J. L., DOW R. P. Poliomyelitis in Hidalgo County, Texas, 1948; poliomyelitis and Coxsackie viruses from flies. Am J Hyg. 1953 Nov;58(3):288–309. doi: 10.1093/oxfordjournals.aje.a119606. [DOI] [PubMed] [Google Scholar]
- MELNICK J. L. Isolation of poliomyelitis virus from single species of flies collected during an urban epidemic. Am J Hyg. 1949 Jan;49(1):8–16. doi: 10.1093/oxfordjournals.aje.a119262. [DOI] [PubMed] [Google Scholar]
- MELNICK J. L., PENNER L. R. The survival of poliomyelitis and Coxsackie viruses following their ingestion by flies. J Exp Med. 1952 Sep;96(3):255–271. doi: 10.1084/jem.96.3.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paul J. R., Trask J. D., Bishop M. B., Melnick J. L., Casey A. E. THE DETECTION OF POLIOMYELITIS VIRUS IN FLIES. Science. 1941 Oct 24;94(2443):395–396. doi: 10.1126/science.94.2443.395. [DOI] [PubMed] [Google Scholar]
- Sabin A. B., Ward R. FLIES AS CARRIERS OF POLIOMYELITIS VIRUS IN URBAN EPIDEMICS. Science. 1941 Dec 19;94(2451):590–591. doi: 10.1126/science.94.2451.590. [DOI] [PubMed] [Google Scholar]
- Sabin A. B., Ward R. INSECTS AND EPIDEMIOLOGY OF POLIOMYELITIS. Science. 1942 Mar 20;95(2464):300–301. doi: 10.1126/science.95.2464.300. [DOI] [PubMed] [Google Scholar]
- Trask J. D., Paul J. R., Melnick J. L., Technical Assistance of John T. Riordan and Marshall Bishop THE DETECTION OF POLIOMYELITIS VIRUS IN FLIES COLLECTED DURING EPIDEMICS OF POLIOMYELITIS : I. METHODS, RESULTS, AND TYPES OF FLIES INVOLVED. J Exp Med. 1943 Jun 1;77(6):531–544. doi: 10.1084/jem.77.6.531. [DOI] [PMC free article] [PubMed] [Google Scholar]