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. 1961 Mar 31;113(4):735–757. doi: 10.1084/jem.113.4.735

TRITON HYPERLIPEMIA IN DOGS

I. IN VITRO EFFECTS OF THE DETERGENT ON SERUM LIPOPROTEINS AND CHYLOMICRONS

Angelo Scanu 1, Pasquale Oriente 1
PMCID: PMC2137371  PMID: 13747054

Abstract

Triton WR-1339, a non-ionic detergent, added to canine serum or to ultracentrifugally separated lipoproteins, induced changes in the lipoproteins which were dependent upon concentration of detergent and class of lipoproteins. D 1.063 to 1.21 lipoprotein (α-LP) was especially sensitive to the action of triton. Addition of 2 mg. of triton to 1 mg. of α-LP (based on protein content), induced only slight changes in the electrophoretic and flotation characteristics of the lipoprotein. With a tenfold increase of the detergent (triton:α-LP, 20:1), the mixture, analyzed by starch gel and paper electrophoresis, yielded a tritonlipid complex which remained close to the origin, and a nearly lipid-free protein with electrophoretic mobility higher (starch gel) or lower (paper) than native α-LP. The splitting of the lipid and protein moieties of α-LP could not be clearly shown when the same mixture was analyzed by free boundary electrophoresis. Triton alone moved only slightly in an electrical field (paper, starch gel, Tiselius); it sedimented during ultracentrifugation at D 1.006 and D 1.063 and floated at D 1.21. D 1.006 to 1.063 lipoproteins (β-LP), required larger amounts of triton to show changes. These were evident in 40 to 80:1 mixtures of triton and β-LP. In starch gel and paper electrophoresis triton retained, in a position close to the origin, part of the lipids of β-LP; the remaining β-LP fraction, impoverished of lipids, had electrophoretic mobility similar to native β-LP. The triton-lipid complex sedimented at D 1.063. After addition of triton to complexes [chylomicron-α-P-I131] or [lipomul-α-LP-1131], the electrophoretic and ultracentrifugal analyses of these mixtures revealed that the labeled protein was removed from the triglyceride component. Triton also prevented the occurrence of the interaction between lipomul and α-LP and the hydrolysis of both chylomicrons and lipomul-α-LP by lipoprotein lipase. It is postulated that, if the changes in lipoproteins and chylomicrons observed in vitro occur in vivo, they could account, at least in part, for the hyperlipemia which develops in animals following administration of triton.

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Selected References

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  1. ABEL L. L., LEVY B. B., BRODIE B. B., KENDALL F. E. A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J Biol Chem. 1952 Mar;195(1):357–366. [PubMed] [Google Scholar]
  2. AVIGAN J., REDFIELD R., STEINBERG D. N-terminal residues of serum lipoproteins. Biochim Biophys Acta. 1956 Jun;20(3):557–558. doi: 10.1016/0006-3002(56)90354-7. [DOI] [PubMed] [Google Scholar]
  3. AYRAULT-JARRIER M., WALD R., POLONOVSKI J. [Influence of detergents on the electrophoretic mobility of isolated serum lipoproteins]. Bull Soc Chim Biol (Paris) 1959;41:753–760. [PubMed] [Google Scholar]
  4. BROWN R. K., BOYLE E., ANFINSEN C. B. The enzymatic transformation of lipoproteins. J Biol Chem. 1953 Sep;204(1):423–434. [PubMed] [Google Scholar]
  5. DELALLA L., LEVINE L., BROWN R. K. Immunologic studies of human high density lipoproteins. J Exp Med. 1957 Aug 1;106(2):261–271. doi: 10.1084/jem.106.2.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DOLE V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest. 1956 Feb;35(2):150–154. doi: 10.1172/JCI103259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FLORSHEIM W. H., MORTON M. E. Stability of phospholipid binding in human serum lipoproteins. J Appl Physiol. 1957 Mar;10(2):301–304. doi: 10.1152/jappl.1957.10.2.301. [DOI] [PubMed] [Google Scholar]
  8. FRIEDMAN M., BYERS S. O. Mechanism underlying hypercholesteremia induced by triton WR-1339. Am J Physiol. 1957 Sep;190(3):439–445. doi: 10.1152/ajplegacy.1957.190.3.439. [DOI] [PubMed] [Google Scholar]
  9. FRIEDMAN M., BYERS S. O. The mechanism responsible for the hypercholesteremia induced by triton WR-1339. J Exp Med. 1953 Jan;97(1):117–130. doi: 10.1084/jem.97.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GOFMAN J. W., LINDGREN F. The role of lipids and lipoproteins in atherosclerosis. Science. 1950 Feb 17;111(2877):166–171. doi: 10.1126/science.111.2877.166. [DOI] [PubMed] [Google Scholar]
  11. GORDON R. S., Jr Interaction between oleate and the lipoproteins of human serum. J Clin Invest. 1955 Mar;34(3):477–484. doi: 10.1172/JCI103097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HIRSCH R. L., KELLNER A. The pathogenesis of hyperlipemia induced by means of surface-active agents. I. Increased total body cholesterol in mice given triton WR 1339 parenterally. J Exp Med. 1956 Jul 1;104(1):1–13. doi: 10.1084/jem.104.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HIRSCH R. L., KELLNER A. The pathogenesis of hyperlipemia induced by means of surface-active agents. II. Failure of exchange of cholesterol between the plasma and the liver in rabbits given triton WR 1339. J Exp Med. 1956 Jul 1;104(1):15–24. doi: 10.1084/jem.104.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. JARRIGE P., LAFOSCADE G. [Elution of proteins after separation by electrophoresis on starch gel]. Bull Soc Chim Biol (Paris) 1959;41:1197–1206. [PubMed] [Google Scholar]
  16. KELLNER A., CORRELL J. W., LADD A. T. Sustained hyperlipemia induced in rabbits by means of intravenously injected surface-active agents. J Exp Med. 1951 Apr 1;93(4):373–384. doi: 10.1084/jem.93.4.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. KORN E. D. Clearing factor, a heparin-activated lipoprotein lipase. II. Substrate specificity and activation of coconut oil. J Biol Chem. 1955 Jul;215(1):15–26. [PubMed] [Google Scholar]
  18. LEVINE L., KAUFFMAN D. L., BROWN R. K. The antigenic similarity of human low density lipoproteins. J Exp Med. 1955 Aug 1;102(2):105–118. doi: 10.1084/jem.102.2.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LEWIS L. A., GREEN A. A., PAGE I. H. Ultracentrifuge lipoprotein pattern of serum of normal, hypertensive and hypothyroid animals. Am J Physiol. 1952 Nov;171(2):391–400. doi: 10.1152/ajplegacy.1952.171.2.391. [DOI] [PubMed] [Google Scholar]
  20. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  21. PETHICA B. A., SCHULMAN J. H. The physical chemistry of haemolysis by surface-active agents. Biochem J. 1953 Jan;53(2):177–185. doi: 10.1042/bj0530177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. POULIK M. D. Starch gel electrophoresis in a discontinous system of buffers. Nature. 1957 Dec 28;180(4600):1477–1479. doi: 10.1038/1801477a0. [DOI] [PubMed] [Google Scholar]
  23. SCANU A., HUGHES W. L. Recombining capacity toward lipids of the protein moiety of human serum alpha 1-lipoprotein. J Biol Chem. 1960 Oct;235:2876–2883. [PubMed] [Google Scholar]
  24. SCANU A., LEWIS L. A., BUMPUS F. M. Separation and characterization of the protein moiety of human alpha1-lipoprotein. Arch Biochem Biophys. 1958 Apr;74(2):390–397. doi: 10.1016/0003-9861(58)90009-2. [DOI] [PubMed] [Google Scholar]
  25. SCANU A., LEWIS L. A., PAGE I. H. Studies on the antigenicity of beta- and alpha-lipoproteins of human serum. J Exp Med. 1958 Aug 1;108(2):185–196. doi: 10.1084/jem.108.2.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. SCHOTZ M. C., SCANU A., PAGE I. H. Effect of triton on lipoprotein lipase of rat plasma. Am J Physiol. 1957 Feb;188(2):399–402. doi: 10.1152/ajplegacy.1957.188.2.399. [DOI] [PubMed] [Google Scholar]
  27. SHORE B. C-and N-terminal amino acids of human serum lipoproteins. Arch Biochem Biophys. 1957 Sep;71(1):1–10. doi: 10.1016/0003-9861(57)90002-4. [DOI] [PubMed] [Google Scholar]
  28. SMITHIES O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J. 1955 Dec;61(4):629–641. doi: 10.1042/bj0610629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. VAN HANDEL E., ZILVERSMIT D. B. Micromethod for the direct determination of serum triglycerides. J Lab Clin Med. 1957 Jul;50(1):152–157. [PubMed] [Google Scholar]

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