Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1961 Mar 31;113(4):713–734. doi: 10.1084/jem.113.4.713

THE INACTIVATION OF ENTEROVIRUS INFECTIVITY BY THE SULFHYDRYL REAGENT p-CHLOROMERCURIBENZOATE

Purnell W Choppin 1, Lennart Philipson 1
PMCID: PMC2137380  PMID: 13693271

Abstract

The infectivity of several enteroviruses was inactivated by the sulfhydryl reagent p-chloromercuribenzoate (PCMB). The rate of inactivation was dependent on the ionic environment in which the reaction was carried out. Inactivation of infectivity was reversed by the thiol compound, reduced glutathione. Under certain conditions, PCMB prevented the adsorption of some enteroviruses to monolayer cultures of monkey kidney cells. The results suggest that enterovirus sulfhydryl groups are involved in the establishment of infection, and that they play a role in the adsorption of virus to host cells.

Full Text

The Full Text of this article is available as a PDF (1.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLISON A. C., VALENTINE R. C. Virus particle adsorption, III. Adsorption of viruses by cell monolayers and effects of some variables on adsorption. Biochim Biophys Acta. 1960 Jun 3;40:400–410. doi: 10.1016/0006-3002(60)91380-9. [DOI] [PubMed] [Google Scholar]
  2. CECIL R., McPHEE J. R. The sulfur chemistry of proteins. Adv Protein Chem. 1959;14:255–389. doi: 10.1016/s0065-3233(08)60613-0. [DOI] [PubMed] [Google Scholar]
  3. CHOPPIN P. W., OSTERHOUT S., TAMM I. Immunological characteristics of N.Y. strains of influenza A virus from the 1957 pandemic. Proc Soc Exp Biol Med. 1958 Jul;98(3):513–520. doi: 10.3181/00379727-98-24092. [DOI] [PubMed] [Google Scholar]
  4. CHOPPIN P. W., TAMM I. Two kinds of particles with contrasting properties in influenza A virus strains from the 1957 pandemic. Virology. 1959 Aug;8:539–542. doi: 10.1016/0042-6822(59)90059-5. [DOI] [PubMed] [Google Scholar]
  5. Choppin P. W., Tamm I. STUDIES OF TWO KINDS OF VIRUS PARTICLES WHICH COMPRISE INFLUENZA A2 VIRUS STRAINS : I. CHARACTERIZATION OF STABLE HOMOGENEOUS SUBSTRAINS IN REACTIONS WITH SPECIFIC ANTIBODY, MUCOPROTEIN INHIBITORS, AND ERYTHROCYTES. J Exp Med. 1960 Oct 31;112(5):895–920. doi: 10.1084/jem.112.5.895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. EAGLE H. The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure. J Exp Med. 1955 Nov 1;102(5):595–600. doi: 10.1084/jem.102.5.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EGGERS H. J., SABIN A. B. Factors determining pathogenicity of variants of ECHO 9 virus for newborn mice. J Exp Med. 1959 Dec 1;110:951–967. doi: 10.1084/jem.110.6.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HANKS J. H., WALLACE R. E. Relation of oxygen and temperature in the preservation of tissues by refrigeration. Proc Soc Exp Biol Med. 1949 Jun;71(2):196–200. doi: 10.3181/00379727-71-17131. [DOI] [PubMed] [Google Scholar]
  10. HOLLAND J. J., McLAREN L. C., SYVERTON J. T. Mammalian cell-virus relationship. III. Poliovirus production by non-primate cells exposed to poliovirus ribonucleic acid. Proc Soc Exp Biol Med. 1959 Apr;100(4):843–845. doi: 10.3181/00379727-100-24798. [DOI] [PubMed] [Google Scholar]
  11. HOLLAND J. J., McLAREN L. C., SYVERTON J. T. The mammalian cell-virus relationship. IV. Infection of naturally insusceptible cells with enterovirus ribonucleic acid. J Exp Med. 1959 Jul 1;110(1):65–80. doi: 10.1084/jem.110.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HOLLAND J. J., McLAREN L. C. The mammalian cell-virus relationship. II. Adsorption, reception, and eclipse of poliovirus by HeLa cells. J Exp Med. 1959 May 1;109(5):487–504. doi: 10.1084/jem.109.5.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. JENSEN E. V. Sulfhydryl-disulfide interchange. Science. 1959 Nov 13;130(3385):1319–1323. doi: 10.1126/science.130.3385.1319. [DOI] [PubMed] [Google Scholar]
  14. McLAREN L. C., HOLLAND J. J., SYVERTON J. T. The mammalian cell-virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non-primate origin. J Exp Med. 1959 May 1;109(5):475–485. doi: 10.1084/jem.109.5.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McLaren L. C., Holland J. J., Syverton J. T. THE MAMMALIAN CELL-VIRUS RELATIONSHIP : V. SUSCEPTIBILITY AND RESISTANCE OF CELLS IN VITRO TO INFECTION BY COXSACKIE A9 VIRUS. J Exp Med. 1960 Sep 30;112(4):581–594. doi: 10.1084/jem.112.4.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PEREZ J. E., BARALT-PEREZ J., KLEIN M. The reversal in vivo by BAL of HgCI2-inactivated influenza A virus in the chick embryo. J Immunol. 1949 Aug;62(4):405–413. [PubMed] [Google Scholar]
  17. PHILIPSON L., CHOPPIN P. W. On the role of virus sulfhydryl groups in the attachment of enteroviruses to erythrocytes. J Exp Med. 1960 Sep 1;112:455–478. [PubMed] [Google Scholar]
  18. PHILIPSON L. Separation on DEAE cellulose of components associated with adenovirus reproduction. Virology. 1960 Apr;10:459–465. doi: 10.1016/0042-6822(60)90129-x. [DOI] [PubMed] [Google Scholar]
  19. PHILIPSON L. Studies on the mechanism of haemagglutination by Echo-viruses. I. Evidence for enzymatic degradation by chymotrypsin of the receptor for Echo-viruses. Arch Gesamte Virusforsch. 1959;9:251–260. doi: 10.1007/BF01240722. [DOI] [PubMed] [Google Scholar]
  20. TAMM I. Enhancement of influenza virus multiplication by 5-methyl-2-D-ribobenzimidazole. Virology. 1956 Aug;2(4):517–531. doi: 10.1016/0042-6822(56)90008-3. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES