Abstract
1. In guinea pigs, aminomethylpteroylglutamic acid (methotrexate) is capable of blocking the development of delayed skin hypersensitivity, the primary antibody response, and the specific febrile response to ovalbumin and diphtheria toxoid. The primary antibody response is more easily inhibited than is the development of delayed skin hypersensitivity. 2. The effect of methotrexate on immunologic responses depended upon the dose of methotrexate employed and the strength of the antigenic stimulus.
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Selected References
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- ALGIRE G. H., WEAVER J. M., PREHN R. T. Growth of cells in vivo in diffusion chambers. I. Survival of homografts in immunized mice. J Natl Cancer Inst. 1954 Dec;15(3):493–507. [PubMed] [Google Scholar]
- DUTTON R. W., DUTTON A. H., GEORGE M. Effect of 8-azaguanine on antibody synthesis in vitro. Nature. 1958 Nov 15;182(4646):1377–1378. doi: 10.1038/1821377a0. [DOI] [PubMed] [Google Scholar]
- DUTTON R. W., DUTTON A. H., VAUGHAN J. H. The effect of 5-bromouracil deoxyriboside on the synthesis of antibody in vitro. Biochem J. 1960 May;75:230–235. doi: 10.1042/bj0750230. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAAS V. H., STEWART S. E. Sparing effect of A-methopterin and guanazolo in mice infected with virus of lymphocytic choriomeningitis. Virology. 1956 Aug;2(4):511–516. doi: 10.1016/0042-6822(56)90007-1. [DOI] [PubMed] [Google Scholar]
- HUENNEKENS F. M., OSBORN M. J., WHITELEY H. R. Folic acid coenzymes. Science. 1958 Jul 18;128(3316):120–124. doi: 10.1126/science.128.3316.120. [DOI] [PubMed] [Google Scholar]
- LERNER E. M., 2nd, HAAS V. H. Histopathology of lymphocytic choriomeningitis in mice spared by amethopterin. Proc Soc Exp Biol Med. 1958 Jun;98(2):395–399. doi: 10.3181/00379727-98-24055. [DOI] [PubMed] [Google Scholar]
- LITTLE P. A., OLESON J. J., ROESCH P. K. The effect of pteroylglutamic acid on some immune responses of chicks. J Immunol. 1950 Nov;65(5):491–498. [PubMed] [Google Scholar]
- LUDOVICI P. P., AXELROD A. E. Circulating antibodies in vitamin-deficiency states; pteroylglutamic acid, niacin-tryptophan, vitamins B12, A, and D deficiencies. Proc Soc Exp Biol Med. 1951 Jul;77(3):526–530. doi: 10.3181/00379727-77-18836. [DOI] [PubMed] [Google Scholar]
- MALMGREN R. A., BENNISON B. E., McKINLEY T. W. The effect of guanazolo on antibody formation. J Natl Cancer Inst. 1952 Feb;12(4):807–818. [PubMed] [Google Scholar]
- MEEKER W. R., Jr, CONDIE R. M., GOOD R. A., VARCO R. L. Alteration of the homograft response by antimetabolites. Ann N Y Acad Sci. 1960 May 31;87:203–213. doi: 10.1111/j.1749-6632.1960.tb23193.x. [DOI] [PubMed] [Google Scholar]
- POTTER M., HAAS V. H. Relationships between lymphocytic choriomeningitis virus, amethopterin, and an amethopterin-resistent lymphocytic neoplasm in mice. J Natl Cancer Inst. 1959 Apr;22(4):801–809. [PubMed] [Google Scholar]
- SALVIN S. B. Occurrence of delayed hypersensitivity during the development of Arthus type hypersensitivity. J Exp Med. 1958 Jan 1;107(1):109–124. doi: 10.1084/jem.107.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALVIN S. B., SMITH R. F. Delayed hypersensitivity in the development of circulating antibody; the effect of x-irradiation. J Exp Med. 1959 Apr 1;109(4):325–338. doi: 10.1084/jem.109.4.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHWARTZ R., STACK J., DAMESHEK W. Effect of 6-mercaptopurine on antibody production. Proc Soc Exp Biol Med. 1958 Oct;99(1):164–167. doi: 10.3181/00379727-99-24281. [DOI] [PubMed] [Google Scholar]
- UHR J. W., BRANDRISS M. W. Delayed hypersensitivity. IV. Systemic reactivity of guinea pigs sensitized to protein antigens. J Exp Med. 1958 Dec 1;108(6):905–924. doi: 10.1084/jem.108.6.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
- UPHOFF D. E. Alteration of homograft reaction by A-methopterin in lethally irradiated mice treated with homologous marrow. Proc Soc Exp Biol Med. 1958 Dec;99(3):651–653. doi: 10.3181/00379727-99-24450. [DOI] [PubMed] [Google Scholar]
- WERTMANN K., CRISLEY F. D., SARANDRIA J. L. Complement-fixing murine typhus antibodies in vitamin deficiency states. III. Riboflavin and folic acid deficiencies. Proc Soc Exp Biol Med. 1952 Jul;80(3):404–406. doi: 10.3181/00379727-80-19638. [DOI] [PubMed] [Google Scholar]