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. 1961 Sep 1;114(3):385–398. doi: 10.1084/jem.114.3.385

IMMUNOCHEMICAL STUDIES OF TWENTY MOUSE MYELOMA PROTEINS: EVIDENCE FOR TWO GROUPS OF PROTEINS SIMILAR TO GAMMA AND BETA-2A GLOBULINS IN MAN

John L Fahey 1
PMCID: PMC2137465  PMID: 13697902

Abstract

The serum myeloma proteins associated with 20 mouse plasma cell tumors in C3H or BALB/c mice that had proved transplantable were characterized by electrophoretic and immunochemical techniques. Although the myeloma proteins ranged in electrophoretic mobility from gamma to alpha globulins, they could be divided into two groups, the gamma type and the beta type myeloma globulins, on the basis of characteristic immunochemical properties. Gamma type myeloma proteins (5563, MPC-11) showed a close immunochemical relationship to normal mouse gamma globulins. Eighteen beta type mouse myeloma proteins migrated as beta or alpha globulins on zone electrophoresis. These proteins shared common antigenic features which permitted their recognition, separate from gamma myeloma proteins. The beta type myeloma proteins were shown to be related to a beta globulin component present in normal serum. Strain differences were observed for the normal beta globulin component believed to be formed in plasma cells. The proteins formed in mouse plasma cells were found to be antigenically complex. Shared antigenic determinants as well as distinctive antigenic determinants were detected when representative myeloma proteins were purified and compared by the Ouchterlony double diffusion technique. The myeloma proteins associated with each of the transplantable plasma cell tumors in mice are regarded as distinctive and characteristic products of plasma cell metabolism. The variety of myeloma globulins was similar for plasma cell tumors arising in C3H as well as in BALB/c mice, indicating that differences in mouse strains would not account for the differences among the myeloma globulins. These differences, however, may be due to differences among the normal plasma cells from which the malignant cells are derived. If this is so, the variety of myeloma globulins reflect the variety of plasma cells present normally.

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Selected References

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  1. CLAUSEN J., HEREMANS J., HEREMANS M. T., RASK-NIELSEN R. Immunoelectrophoretic studies of serums from mice carrying two transplantable plasma-cell leukemias. J Natl Cancer Inst. 1959 Jan;22(1):57–81. [PubMed] [Google Scholar]
  2. FAHEY J. L., HORBETT A. P. Human gamma globulin fractionation on anion exchange cellulose columns. J Biol Chem. 1959 Oct;234:2645–2651. [PubMed] [Google Scholar]
  3. FAHEY J. L., POTTER M. Bence Jones proteinuria associated with a transplantable mouse plasma-cell neoplasm. Nature. 1959 Aug 22;184(Suppl 9):654–655. doi: 10.1038/184654a0. [DOI] [PubMed] [Google Scholar]
  4. FAHEY J. L., POTTER M., GUTTER F. J., DUNN T. B. Distinctive myeloma globulins associated with a new plasma cell neoplasm of strain C3H mice. Blood. 1960 Jan;15:103–113. [PubMed] [Google Scholar]
  5. GRABAR P., FAUVERT R., BURTIN P., HARTMANN L. Etude sur les protéines du myélome. II. L'analyse immuno-électrophorétique des sérums de 30 malades. Rev Fr Etud Clin Biol. 1956 Feb;1(2):175–187. [PubMed] [Google Scholar]
  6. KORNGOLD L., LIPARI R. Multiple-myeloma proteins. I. Immunological studies. Cancer. 1956 Jan-Feb;9(1):183–192. doi: 10.1002/1097-0142(195601/02)9:1<183::aid-cncr2820090119>3.0.co;2-x. [DOI] [PubMed] [Google Scholar]
  7. MERWIN R. M., ALGIRE G. H. Induction of plasma-cell neoplasms and fibrosarcomas in BALB/c mice carrying diffusion chambers. Proc Soc Exp Biol Med. 1959 Jul;101(3):437–439. doi: 10.3181/00379727-101-24970. [DOI] [PubMed] [Google Scholar]
  8. MULLER-EBERHARD H. J., KUNKEL H. G. The carbohydrate of gamma-globulin and myeloma proteins. J Exp Med. 1956 Aug 1;104(2):253–269. doi: 10.1084/jem.104.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. OSSERMAN E. F. A modified technique of immunoelectrophoresis facilitating the identification of specific precipitin arcs. J Immunol. 1960 Jan;84:93–97. [PubMed] [Google Scholar]
  10. POTTER M., FAHEY J. L., PILGRIM H. I. Abnormal serum protein and bone destruction in transmissible mouse plasma cell neoplasm (multiple myeloma). Proc Soc Exp Biol Med. 1957 Feb;94(2):327–333. doi: 10.3181/00379727-94-22035. [DOI] [PubMed] [Google Scholar]
  11. POTTER M., FAHEY J. L. Studies on eight transplantable plasma-cell neoplasms of mice. J Natl Cancer Inst. 1960 May;24:1153–1165. [PubMed] [Google Scholar]
  12. RASK-NIELSEN R., GORMSEN H., CLAUSEN J. A transplantable plasma-cell leukemia in mice associated with the production of beta-paraprotein. J Natl Cancer Inst. 1959 Mar;22(3):509–541. [PubMed] [Google Scholar]
  13. SLATER R. J., WARD S. M., KUNKEL H. G. Immunological relationships among the myeloma proteins. J Exp Med. 1955 Jan 1;101(1):85–108. doi: 10.1084/jem.101.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WIEME R. J. An improved technique of agar-gel electrophoresis on microscope slides. Clin Chim Acta. 1959 May;4(3):317–321. doi: 10.1016/0009-8981(59)90096-8. [DOI] [PubMed] [Google Scholar]

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