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. 1962 Jan 1;115(1):1–12. doi: 10.1084/jem.115.1.1

OBSERVATIONS ON THE FINE STRUCTURE OF MATURE HERPES SIMPLEX VIRUS AND ON THE COMPOSITION OF ITS NUCLEOID

M A Epstein 1
PMCID: PMC2137471  PMID: 13890435

Abstract

The fine structure and composition of mature herpes virus have been investigated in thin sections by electron microscopy. The virus was grown in cultured HeLa cells and was collected with them. Tests for the biological activity of the infected cultures were included in the first half of the work. Preparations were fixed with both osmium and permanganate, and were embedded either in methacrylate or in aquon, a water-miscible fraction of a commercially available epoxy resin. In further experiments material fixed with permanganate was subjected to the action of specific nucleases or control medium before embedding. All the preparations showed numerous uniform particles around and between the cells and this was paralleled by considerable biological activity where tests were made on samples of the culture fluids. Mature herpes virus has been found to be round and to measure, when dehydration shrinkage is avoided, about 165 mµ in diameter. The particle contained an eccentric round-ended rod-shaped, electron-opaque nucleoid lying in an inner zone of low density. A dense outer zone or viroplasm surrounded this, no membrane being present between the two zones. After permanganate fixation the particle was found to have an outer limiting membrane showing a triple-layered structure morphologically indistinguishable from that of the plasma membrane of the HeLa cells. The results of the digestion experiments show that herpes virus contains nucleic acid of deoxyribose type and that this is localized in the dense nucleoid. Both the findings and the methods used are discussed.

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Selected References

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  1. BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
  2. CROUSE H. V., CORIELL L. L., BLANK H., SCOTT T. F. M. Cytochemical studies on the intranuclear inclusion of herpes simplex. J Immunol. 1950 Jul;65(1):119–128. [PubMed] [Google Scholar]
  3. EPSTEIN M. A. A diamond knife holder for microtomes designed to take glass knives. J Biophys Biochem Cytol. 1958 Sep 25;4(5):539–540. doi: 10.1083/jcb.4.5.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EPSTEIN M. A. An investigation into the purifying effect of a fluorocarbon on vaccinia virus. Br J Exp Pathol. 1958 Aug;39(4):436–446. [PMC free article] [PubMed] [Google Scholar]
  5. EPSTEIN M. A. Composition of the Rous virus nucleoid. Nature. 1958 Jun 28;181(4626):1808–1808. doi: 10.1038/1811808a0. [DOI] [PubMed] [Google Scholar]
  6. EPSTEIN M. A. Constitution of tumor viruses. Natl Cancer Inst Monogr. 1960 Sep;4:53–65. [PubMed] [Google Scholar]
  7. EPSTEIN M. A., HOLT S. J. Observations on the Rous virus; integrated electron microscopical and cytochemical studies of flucrocarbon purified preparations. Br J Cancer. 1958 Sep;12(3):363–369. doi: 10.1038/bjc.1958.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EPSTEIN M. A., HOLT S. J., POWELL A. K. The fine structure and composition of type 5 adenovirus; an integrated electron microscopical and cytochemical study. Br J Exp Pathol. 1960 Dec;41:567–576. [PMC free article] [PubMed] [Google Scholar]
  9. EPSTEIN M. A., HOLT S. J. Site and nature of adenovirus nucleic acid. Nature. 1960 Sep 17;187:1050–1051. doi: 10.1038/1871050a0. [DOI] [PubMed] [Google Scholar]
  10. EPSTEIN M. A. Observations on the Rous virus; purification and identification of the particles from solid tumours. Br J Cancer. 1958 Jun;12(2):248–255. doi: 10.1038/bjc.1958.30. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. EPSTEIN M. A. Observations on the fine structure of type 5 adenovirus. J Biophys Biochem Cytol. 1959 Dec;6:523–526. doi: 10.1083/jcb.6.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. EPSTEIN M. A., POWELL A. K. The isolation of type 5 adenovirus using a fluorocarbon; combined morphological and biological studies. Br J Exp Pathol. 1960 Dec;41:559–566. [PMC free article] [PubMed] [Google Scholar]
  13. EPSTEIN M. A. Some unusual features of fine structure observed in HeLa cells. J Biophys Biochem Cytol. 1961 Jun;10:153–162. doi: 10.1083/jcb.10.2.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. FARNHAM A. E., NEWTON A. A. The effect of some environmental factors on herpes virus grown in HeLa cells. Virology. 1959 Apr;7(4):449–461. doi: 10.1016/0042-6822(59)90073-x. [DOI] [PubMed] [Google Scholar]
  15. FINCK H. Epoxy resins in electron microscopy. J Biophys Biochem Cytol. 1960 Feb;7:27–30. doi: 10.1083/jcb.7.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. GIBBONS I. R. An embedding resin miscible with water for electron microscopy. Nature. 1959 Aug 1;184(Suppl 6):375–376. doi: 10.1038/184375b0. [DOI] [PubMed] [Google Scholar]
  17. LUFT J. H. Permanganate; a new fixative for electron microscopy. J Biophys Biochem Cytol. 1956 Nov 25;2(6):799–802. doi: 10.1083/jcb.2.6.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MORGAN C., ELLISON S. A., ROSE H. M., MOORE D. H. Structure and development of viruses as observed in the electron microscope. I. Herpes simplex virus. J Exp Med. 1954 Aug 1;100(2):195–202. doi: 10.1084/jem.100.2.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MORGAN C., ROSE H. M., HOLDEN M., JONES E. P. Electron microscopic observations on the development of herpes simplex virus. J Exp Med. 1959 Oct 1;110:643–656. doi: 10.1084/jem.110.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. NEWTON A., STOKER M. G. Changes in nucleic acid content of HeLa cells infected with herpes virus. Virology. 1958 Jun;5(3):549–560. doi: 10.1016/0042-6822(58)90044-8. [DOI] [PubMed] [Google Scholar]
  21. PALAY S. L., KARLIN L. J. An electron microscopic study of the intestinal villus. I. The fasting animal. J Biophys Biochem Cytol. 1959 May 25;5(3):363–372. doi: 10.1083/jcb.5.3.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. PEACHEY L. D. A device for staining tissue sections for electron microscopy. J Biophys Biochem Cytol. 1959 May 25;5(3):511–513. doi: 10.1083/jcb.5.3.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. PEREIRA H. G., KELLY B. Dose-response curves of toxic and infective actions of adenovirus in HeLa cell cultures. J Gen Microbiol. 1957 Oct;17(2):517–524. doi: 10.1099/00221287-17-2-517. [DOI] [PubMed] [Google Scholar]
  24. REISSIG M., MELNICK J. L. The cellular changes produced in tissue cultures by herpes B virus correlated with the concurrent multiplication of the virus. J Exp Med. 1955 Mar 1;101(3):341–352. doi: 10.1084/jem.101.3.341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. ROBERTSON J. D. The ultrastructure of cell membranes and their derivatives. Biochem Soc Symp. 1959;16:3–43. [PubMed] [Google Scholar]
  26. STOKER M. G., SMITH K. M., ROSS R. W. Electron microscope studies of HeLa cells infected with herpes virus. J Gen Microbiol. 1958 Oct;19(2):244–249. doi: 10.1099/00221287-19-2-244. [DOI] [PubMed] [Google Scholar]
  27. TAMM I., BABLANIAN R. On the role of ribonucleic acid in animal virus synthesis. II. Studies with ribonuclease. J Exp Med. 1960 Mar 1;111:351–368. doi: 10.1084/jem.111.3.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. WILDY P., RUSSELL W. C., HORNE R. W. The morphology of herpes virus. Virology. 1960 Oct;12:204–222. doi: 10.1016/0042-6822(60)90195-1. [DOI] [PubMed] [Google Scholar]

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