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. 1962 Jan 1;115(1):63–82. doi: 10.1084/jem.115.1.63

THE LOCALIZATION OF IN VIVO BOUND COMPLEMENT IN TISSUE SECTIONS

P J Lachmann 1, H J Müller-Eberhard 1, H G Kunkel 1, F Paronetto 1
PMCID: PMC2137475  PMID: 14461382

Abstract

A technique has been described for the demonstration of a human complement component by an immunofluorescent method. The component detected is β1C-globulin, a moiety of the third complement component, which has previously been obtained in pure form and to which a specific antiserum has been prepared. It has been shown in a model system that the binding of β1C-globulin as shown by immunofluorescence is strictly equivalent to complement fixation as assessed by standard serological methods. This technique has been applied to the detection of in vivo bound complement in pathological human tissues. It was found that in vivo complement binding occurs in the lesions of several human diseases, but not elsewhere in the same tissues. In a rather limited survey of diseases that has been carried out, in vivo complement binding was found particularly in systemic L.E., various nephritides, and amyloidosis, as well as in single cases of some other diseases. The spectrum of in vivo complement binding has been compared with that of γ-globulin binding (7S and 19S types) and with the demonstration of in vitro complement fixation and rheumatoid factor fixation. It was distinct from each of these. Rheumatoid factor fixation, detected by anti-19S antiserum showed promise as a method for the detection of antigen-antibody complexes and aggregated γ-globulin in tissue sections. The interpretation of these findings in regard to the nature of the binding sites, and their possible significance in regard to pathogenic mechanisms have been discussed.

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Selected References

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  1. CHRISTIAN C. L. Studies of aggregated gamma-globulin. I. Sedimentation, electrophoretic and anticomplementary properties. J Immunol. 1960 Jan;84:112–116. [PubMed] [Google Scholar]
  2. CHRISTIAN C. L. Studies of aggregated gamma-globulin. II. Effect in vivo. J Immunol. 1960 Jan;84:117–121. [PubMed] [Google Scholar]
  3. DIXON F. J., VAZQUEZ J. J. Immunohistochemical analysis of amyloid by the fluorescence technique. J Exp Med. 1956 Nov 1;104(5):727–736. doi: 10.1084/jem.104.5.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DIXON F. J., VAZQUEZ J. J., WEIGLE W. O., COCHRANE C. G. Pathogenesis of serum sickness. AMA Arch Pathol. 1958 Jan;65(1):18–28. [PubMed] [Google Scholar]
  5. EDELMAN G. M., KUNKEL H. G., FRANKLIN E. C. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. doi: 10.1084/jem.108.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FREEDMAN P., PETERS J. H., KARK R. M. Localization of gamma-globulin in the diseased kidney. Arch Intern Med. 1960 Apr;105:524–535. doi: 10.1001/archinte.1960.00270160022005. [DOI] [PubMed] [Google Scholar]
  7. GOLDWASSER R. A., SHEPARD C. C. Staining of complement and modification of fluorescent antibody procedures. J Immunol. 1958 Feb;80(2):122–131. [PubMed] [Google Scholar]
  8. Hochwald G. M., Thorbecke G. J., Asofsky R. SITES OF FORMATION OF IMMUNE GLOBULINS AND OF A COMPONENT OF C'(3): I. A NEW TECHNIQUE FOR THE DEMONSTRATION OF THE SYNTHESIS OF INDIVIDUAL SERUM PROTEINS BY TISSUES IN VITRO. J Exp Med. 1961 Sep 30;114(4):459–470. doi: 10.1084/jem.114.4.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. ISHIZAKA K., ISHIZAKA T. Biologic activity of aggregated gamma-globulin. II. A study of various methods for aggregation and species differences. J Immunol. 1960 Aug;85:163–171. [PubMed] [Google Scholar]
  10. ISHIZAKA T., ISHIZAKA K. Biological activities of aggregated gamma globulin. I. Skin reactive and complement-fixing properties of heat denatured gamma globulin. Proc Soc Exp Biol Med. 1959 Aug-Sep;101:845–850. doi: 10.3181/00379727-101-25116. [DOI] [PubMed] [Google Scholar]
  11. KAPLAN M. H., DALLENBACH F. D. Immunologic studies of heart tissue. III. Occurrence of bound gamma globulin in auricular appendages from rheumatic hearts. Relationship to certain histopathologic features of rheumatic heart disease. J Exp Med. 1961 Jan 1;113:1–16. doi: 10.1084/jem.113.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KLEIN P., BURKHOLDER P. [A method for fluorescence-optic demonstration of complement fixation and its use in the histo-immunological demonstration of experimental kidney anaphylaxis]. Dtsch Med Wochenschr. 1959 Nov 6;84:2001–2004. doi: 10.1055/s-0028-1114563. [DOI] [PubMed] [Google Scholar]
  13. MARSHALL J. D., EVELAND W. C., SMITH C. W. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898–900. doi: 10.3181/00379727-98-24222. [DOI] [PubMed] [Google Scholar]
  14. MELLORS R. C., ORTEGA L. G. Analytical pathology. III. New observations on the pathogenesis of glomerulonephritis, lipid nephrosis, periarteritis nodosa, and secondary amyloidosis in man. Am J Pathol. 1956 May-Jun;32(3):455–499. [PMC free article] [PubMed] [Google Scholar]
  15. MELLORS R. C., ORTEGA L. G., HOLMAN H. R. Role of gamma globulins in pathogenesis of renal lesions in systemic lupus erythematosus and chronic membranous glomerulonephritis, with an observation on the lupus erythematosus cell reaction. J Exp Med. 1957 Aug 1;106(2):191–202. doi: 10.1084/jem.106.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MULLER-EBERHARD H. J. Isolation and description of proteins related to the human complement system. Acta Soc Med Ups. 1961;66:152–170. [PubMed] [Google Scholar]
  17. MULLER-EBERHARD H. J., KUNKEL H. G. Isolation of a thermolabile serum protein which precipitates gamma-globulin aggregates and participates in immune hemolysis. Proc Soc Exp Biol Med. 1961 Feb;106:291–295. doi: 10.3181/00379727-106-26313. [DOI] [PubMed] [Google Scholar]
  18. MULLER-EBERHARD H. J., NILSSON U., ARONSSON T. Isolation and characterization of two beta1-glycoproteins of human serum. J Exp Med. 1960 Feb 1;111:201–215. doi: 10.1084/jem.111.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Müller-Eberhard H. J., Nilsson U. RELATION OF A beta(1)-GLYCOPROTEIN OF HUMAN SERUM TO THE COMPLEMENT SYSTEM. J Exp Med. 1960 Jan 31;111(2):217–234. doi: 10.1084/jem.111.2.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. ROSS A., LEPOW I. H. Studies on immune cellular injury. I. Cytotoxic effects of antibody and complement. J Exp Med. 1960 Dec 1;112:1085–1106. doi: 10.1084/jem.112.6.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  22. TURK J. L. Immune-adherence with soluble antigens. Immunology. 1958 Oct;1(4):305–314. [PMC free article] [PubMed] [Google Scholar]
  23. VAZQUEZ J. J., DIXON F. J. Immunohistochemical analysis of lesions associated with fibrinoid change. AMA Arch Pathol. 1958 Oct;66(4):504–517. [PubMed] [Google Scholar]
  24. VOGT A., KOCHEM H. G. [Histo-serological studies with fluorescein-labeled anticomplement. Demonstration of complement-binding substances in amyloid]. Z Zellforsch Mikrosk Anat. 1960;52:640–652. [PubMed] [Google Scholar]

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