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. 1962 Jan 31;115(2):439–452. doi: 10.1084/jem.115.2.439

DESTRUCTION OF EPIDERMAL CELLS IN VITRO BY AUTOLOGOUS SERUM FROM NORMAL ANIMALS

Paul I Terasaki 1, Charles C Chamberlain 1
PMCID: PMC2137489  PMID: 14039682

Abstract

Sera and plasma from normal rats and rabbits were shown to be extremely toxic in vitro to autologous epidermal cells. On the other hand, mouse sera and newborn rat sera were innocuous to autologous epidermal cells. Viability of cells was assessed by the method of eosin dye exclusion upon 2 hour incubation at 37°C. Testicular cells were also killed by autologous sera, but polymorphonuclear leukocytes, lymphocytes, and lymph node cells were not affected. Autotoxicity of sera could be destroyed by the depletion of complement components with an antigen-antibody precipitate, heat, zymosan, and NH3. Moreover, activity of sera could be absorbed out by epidermal cells, though not by lymph node cells or erythrocytes. Such absorption of toxicity was not individual-specific since homologous epidermal cells also absorbed toxicity, and in addition, were killed by fresh normal serum. Enzyme inhibitors such as soybean trypsin inhibitor and ε-amino-n-caproic acid did not affect the activity of fresh autologous serum. It is suggested that a natural barrier exists between the basal cells of the epidermis and the plasma which prevents the autodestructive process under normal conditions. Any injury to this barrier may than lead to necrosis and death of the epidermis as seen in various pathological conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALKJAERSIG N., FLETCHER A. P., SHERRY S. xi-Aminocaproic acid: an inhibitor of plasminogen activation. J Biol Chem. 1959 Apr;234(4):832–837. [PubMed] [Google Scholar]
  2. BILLINGHAM R. E., REYNOLDS J. Transplantation studies on sheets of pure epidermal epithelium and on epidermal cell suspensions. Br J Plast Surg. 1952 Apr;5(1):25–36. doi: 10.1016/s0007-1226(52)80004-9. [DOI] [PubMed] [Google Scholar]
  3. CHYTILOVA M., KULHANEK V. Skin autoantibodies and unsuccessful autotransplantation following immunization with freeze-dried autologous skin. Transplant Bull. 1960 Jul;26:109–110. [PubMed] [Google Scholar]
  4. CODE C. F. Histamine in blood. Physiol Rev. 1952 Jan;32(1):47–65. doi: 10.1152/physrev.1952.32.1.47. [DOI] [PubMed] [Google Scholar]
  5. COFFIELD K. J., SPAET T. H. Studies on the normal serum panagglutinin active against trypsinated human erythrocytes. V. The nonidentity of the agglutinin with serum trypsin inhibitor and the components of complement. J Lab Clin Med. 1959 Dec;54:871–875. [PubMed] [Google Scholar]
  6. DIXON F. J., FELDMAN J. D., VAZQUEZ J. J. Experimental glomerulonephritis. The pathogenesis of a laboratory model resembling the spectrum of human glomerulonephritis. J Exp Med. 1961 May 1;113:899–920. doi: 10.1084/jem.113.5.899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FREUND J., LIPTON M. M., THOMPSON G. E. Aspermatogenesis in the guinea pig induced by testicular tissue and adjuvants. J Exp Med. 1953 May;97(5):711–726. doi: 10.1084/jem.97.5.711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GOLUBOW J., MARTIN C. J., AXELROD A. E. Proteolytic enzyme system of skin. VI. Enzyme patterns in various animal species. Proc Soc Exp Biol Med. 1959 Jan;100(1):142–146. doi: 10.3181/00379727-100-24553. [DOI] [PubMed] [Google Scholar]
  9. HANKS J. H., WALLACE J. H. Determination of cell viability. Proc Soc Exp Biol Med. 1958 May;98(1):188–192. doi: 10.3181/00379727-98-23985. [DOI] [PubMed] [Google Scholar]
  10. HIRSCH J. G. Comparative bactericidal activities of blood serum and plasma serum. J Exp Med. 1960 Jul 1;112:15–22. doi: 10.1084/jem.112.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. ISHIZAKA K., ISHIZAKA T., CAMPBELL D. H. The biological activity of soluble antigen-antibody complexes. II. Physical properties of soluble complexes having skin-irritating activity. J Exp Med. 1959 Feb 1;109(2):127–143. doi: 10.1084/jem.109.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KERBY G. P. Methods for the study of surviving leukocytes. D. Lysozyme technic for measuring cell damage. Methods Med Res. 1958;7:140–141. [PubMed] [Google Scholar]
  13. LANDY M., MICHAEL J. G., TRAPANI R. J., ACHINSTEIN B., WOODS M. W., SHEAR M. J. An antibody-complement system in normal serum lethal to mouse tumor cells. Cancer Res. 1960 Oct;20:1279–1291. [PubMed] [Google Scholar]
  14. PUCK T. T., CIECIURA S. J., ROBINSON A. Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med. 1958 Dec 1;108(6):945–956. doi: 10.1084/jem.108.6.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ROSENTHAL M. C., SCHWARTZ L. I. Reversible agglutination of trypsin treated erythrocytes by normal human sera. Proc Soc Exp Biol Med. 1951 Apr;76(4):635–638. doi: 10.3181/00379727-76-18581. [DOI] [PubMed] [Google Scholar]
  16. ROSENTHAL S. A., BAER R. L., HAGEL B. Failure to sensitize to autologous skin. Proc Soc Exp Biol Med. 1958 Feb;97(2):279–281. doi: 10.3181/00379727-97-23715. [DOI] [PubMed] [Google Scholar]
  17. TERASAKI P. I., ESAIL M. L., CANNON J. A., LONGMIRE W. P., Jr Destruction of lymphocytes in vitro by normal serum from common laboratory animals. J Immunol. 1961 Oct;87:383–395. [PubMed] [Google Scholar]
  18. TERASAKI P. I., McCLELLAND J. D., CANNON J. A., LONGMIRE W. P., Jr Antibody response to homografts. VII. The role of complement in cytotoxicity of serum from homografted rabbits. J Immunol. 1961 Jul;87:39–45. [PubMed] [Google Scholar]
  19. UNGAR G., HAYASHI H. Enzymatic mechanisms in allergy. Ann Allergy. 1958 Sep-Oct;16(5):542–581. [PubMed] [Google Scholar]
  20. VOISIN G., MAURER P. Sur une dermatose expérimentale apparue chez des lapins traités par des extraits de peau homologue. Sem Hop. 1955 May 30;31(33):1909–1912. [PubMed] [Google Scholar]
  21. WAALKES T. P., WEISSBACH H., BOZICEVICH J., UDENFRIEND S. Serotonin and histamine release during anaphylaxis in the rabbit. J Clin Invest. 1957 Jul;36(7):1115–1120. doi: 10.1172/JCI103507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. WEIGLE W. O., COCHRANE C. G., DIXON F. J. Anaphylactogenic properties of soluble antigen-antibody complexes in the guinea pig and rabbit. J Immunol. 1960 Nov;85:469–477. [PubMed] [Google Scholar]
  23. WITEBSKY E., ROSE N. R. Studies on organ specificity. IV. Production of rabbit thyroid antibodies in the rabbit. J Immunol. 1956 Jun;76(6):408–416. [PubMed] [Google Scholar]

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