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. 1962 Feb 28;115(3):671–683. doi: 10.1084/jem.115.3.671

STUDIES ON THE BINDING BETWEEN STREPTOCOCCAL M PROTEIN AND ANTIBODY

Howard M Grey 1
PMCID: PMC2137498  PMID: 13901773

Abstract

A method for detecting type-specific antibody to type 12 β-hemolytic streptococci is described. The method is based on the utilization of a partially purified I131 labeled streptococcal acid extract and the solubility of this extract in 40 per cent saturated ammonium sulfate as compared to the insolubility of antibody and antigen-antibody complexes at this salt concentration. The salient features of this technique are: (a) When absorbed sera were used, no non-type-specific reaction with antigen occurred. (b) The sensitivity of the system allowed detection of type-specific antibody in hyperimmune antisera that had been diluted 1200-fold. (c) The method does not rely on secondary manifestations of antigen-antibody interaction for the detection of antibody. (d) As little as 0.03 µg of M12 protein was detected when soluble unlabeled M protein was used to inhibit the reaction between I131 M12 protein and anti-M12 antibody. (e) The kinetics of the reaction between M protein and anti-M antibody can be studied, thereby making information available as to the quality as well as the quantity of antibody produced in this antigen-antibody system.

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Selected References

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  1. BONE M., BRAUDE A. I., KLEINMAN H. Complement-fixing antibody response to M-protein of nephritogenic streptococci in glomerulonephritis. J Lab Clin Med. 1957 Nov;50(5):705–711. [PubMed] [Google Scholar]
  2. EKSTEDT R. D., STOLLERMAN G. H. Factors affecting the chain length of group A streptococci. II. Quantative M-anti-M relationships in the long chain test. J Exp Med. 1960 Oct 1;112:687–698. doi: 10.1084/jem.112.4.687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
  4. HALBERT S. P., SWICK L., SONN C. The use of precipitin analysis in agar for the study of human streptococcal infections. II. Ouchterlony and Oakley technics. J Exp Med. 1955 May 1;101(5):557–576. doi: 10.1084/jem.101.5.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hirst G. K., Lancefield R. C. ANTIGENIC PROPERTIES OF THE TYPE-SPECIFIC SUBSTANCE DERIVED FROM GROUP A HEMOLYTIC STREPTOCOCCI. J Exp Med. 1939 Feb 28;69(3):425–445. doi: 10.1084/jem.69.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LANCEFIELD R. C., PERLMANN G. E. Preparation and properties of type-specific M antigen isolated from a group A, type 1 hemolytic streptococcus. J Exp Med. 1952 Jul;96(1):71–82. doi: 10.1084/jem.96.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LANNI F., DILLON M. L., BEARD J. W. Determination of small quantities of nitrogen in serological precipitates and other biological materials. Proc Soc Exp Biol Med. 1950 May;74(1):4–7. doi: 10.3181/00379727-74-17791. [DOI] [PubMed] [Google Scholar]
  8. Lancefield R. C. THE ANTIGENIC COMPLEX OF STREPTOCOCCUS HAEMOLYTICUS : I. DEMONSTRATION OF A TYPE-SPECIFIC SUBSTANCE IN EXTRACTS OF STREPTOCOCCUS HAEMOLYTICUS. J Exp Med. 1928 Jan 1;47(1):91–103. doi: 10.1084/jem.47.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lancefield R. C., Todd E. W. ANTIGENIC DIFFERENCES BETWEEN MATT HEMOLYTIC STREPTOCOCCI AND THEIR GLOSSY VARIANTS. J Exp Med. 1928 Nov 30;48(6):769–790. doi: 10.1084/jem.48.6.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. ROTHBARD S. Protective effect of hyaluronidase and type-specific anti-M serum on experimental group A streptococcus infection in mice. J Exp Med. 1948 Sep 1;88(3):325–342. doi: 10.1084/jem.88.3.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rothbard S. BACTERIOSTATIC EFFECT OF HUMAN SERA ON GROUP A STREPTOCOCCI : II. COMPARATIVE BACTERIOSTATIC EFFECT OF NORMAL WHOLE BLOOD FROM DIFFERENT ANIMAL SPECIES IN THE PRESENCE OF HUMAN CONVALESCENT SERA. J Exp Med. 1945 Aug 1;82(2):107–118. doi: 10.1084/jem.82.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Swift H. F., Wilson A. T., Lancefield R. C. TYPING GROUP A HEMOLYTIC STREPTOCOCCI BY M PRECIPITIN REACTIONS IN CAPILLARY PIPETTES. J Exp Med. 1943 Aug 1;78(2):127–133. doi: 10.1084/jem.78.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TALMAGE D. W., BAKER H. R., AKESON W. The separation and analysis of labelled antibodies. J Infect Dis. 1954 Mar-Apr;94(2):199–212. doi: 10.1093/infdis/94.2.199. [DOI] [PubMed] [Google Scholar]
  14. TALMAGE D. W., MAURER P. H. I131-Labelled antigen precipitation as a measure of quantity and quality of antibody. J Infect Dis. 1953 May-Jun;92(3):288–300. doi: 10.1093/infdis/92.3.288. [DOI] [PubMed] [Google Scholar]
  15. TALMAGE D. W. The primary equilibrium between antigen and antibody. Ann N Y Acad Sci. 1957 Aug 30;70(1):82–93. doi: 10.1111/j.1749-6632.1957.tb35380.x. [DOI] [PubMed] [Google Scholar]
  16. Todd E. W., Lancefield R. C. VARIANTS OF HEMOLYTIC STREPTOCOCCI; THEIR RELATION TO TYPE-SPECIFIC SUBSTANCE, VIRULENCE, AND TOXIN. J Exp Med. 1928 Nov 30;48(6):751–767. doi: 10.1084/jem.48.6.751. [DOI] [PMC free article] [PubMed] [Google Scholar]

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