Abstract
Peptides I and II, two metabolically active peptides from porcine pituitary glands, stimulated antibody production in rabbits. The antiserum obtained had antibodies which interacted with peptides I and II from porcine pituitaries, peptides I and II from human pituitaries, and fraction H prepared by Rudman from hog pituitaries. None of the other pituitary preparations tested showed any interaction with peptides I and II. All three preparations stimulated the release of free fatty acids from rabbit adipose tissue in vitro. Antiserum inhibited this biological action to a different extent in each of the three preparations. Following an intravenous injection of peptides I and II there was a rapid rise in the plasma concentration of free fatty acids in rabbits. Injection of antiserum together with peptides I and II failed to block the rapid increase in free fatty acids. However, an injection of at least ten times the minimal amount of peptides I and II which raised the plasma free fatty acid concentration produced no increase in a rabbit with a high antibody titer to these peptides.
Full Text
The Full Text of this article is available as a PDF (939.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ASTWOOD E. B., BARRETT R. J., FRIESEN H. Two metabolically active peptides from porcine pituitary glands. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1525–1530. doi: 10.1073/pnas.47.10.1525. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DI GIROLAMO M., RUDMAN D., REID M. B., SEIDMAN F. Effect of pituitary hormones upon serum free fatty acid concentration of the rabbit. Endocrinology. 1961 Mar;68:457–465. doi: 10.1210/endo-68-3-457. [DOI] [PubMed] [Google Scholar]
- DOLE V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest. 1956 Feb;35(2):150–154. doi: 10.1172/JCI103259. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FERGUSON K. A., WALLACE A. L. Starch-gel electrophoresis of anterior pituitary hormones. Nature. 1961 May 13;190:629–630. doi: 10.1038/190629a0. [DOI] [PubMed] [Google Scholar]
- HAYASHIDA T., LI C. H. A comparative immunological study of pituitary growth hormone from various species. Endocrinology. 1959 Dec;65:944–956. doi: 10.1210/endo-65-6-944. [DOI] [PubMed] [Google Scholar]
- POULIK M. D. Starch gel electrophoresis in a discontinous system of buffers. Nature. 1957 Dec 28;180(4600):1477–1479. doi: 10.1038/1801477a0. [DOI] [PubMed] [Google Scholar]
- READ C. H., BRYAN G. T. The immunological assay of human growth hormone. Recent Prog Horm Res. 1960;16:187–218. [PubMed] [Google Scholar]
- RUDMAN D., REID M. B., SEIDMAN F., DI GIROLAMO M., WERTHEIM A. R., BERN S. Purification and properties of a component of the pituitary gland which produces lipemia in the rabbit. Endocrinology. 1961 Feb;68:273–280. doi: 10.1210/endo-68-2-273. [DOI] [PubMed] [Google Scholar]
- STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]