Abstract
1. If quantities of bovine serum albumin or bovine gamma globulin in the range of 2 to 4 mg are inoculated intracorneally into rabbits, a biphasic reaction occurs in the cornea. 2. The primary reaction, which becomes manifest approximately 3 days after inoculation and lasts several days, is characterized by a diffuse clouding of the cornea. During this period, no antibody can be demonstrated either by serological or histological techniques. The animals react with a delayed type of skin reaction and the sensitivity can be passively transferred to normal guinea pigs with the cells of pooled lymph nodes or buffy coats. The corneal reaction is therefore considered a manifestation of delayed sensitivity. 3. The secondary reaction in the cornea, usually occurring about the 14th day after inoculation is the "Wessely phenomenon." This reaction is characterized by a precipitation of immune complexes in the cornea resulting in a visible annular corneal opacity. Circulating antibody can be readily demonstrated by both serological and histological techniques and the animals demonstrate typical Arthus skin sensitivity. 4. Generally intervening between these two phases of corneal activity is a stage in which the inoculated eye appears essentially normal. This stage is eliminated if both corneas are inoculated initially.
Full Text
The Full Text of this article is available as a PDF (1.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BREEBAART A. C., JAMES-WITTE J. Studies on experimental corneal allergy. Am J Ophthalmol. 1959 Jul;48(1 Pt 1):37–47. doi: 10.1016/0002-9394(59)90283-1. [DOI] [PubMed] [Google Scholar]
- GERMUTH F. G., Jr, MAUMENEE A. E., PRATT-JOHNSON J., SENTERFIT L. B., VAN ARNAM C. E., POLLACK A. D. Observations on the site and mechanisms of antigen-antibody interaction in anaphylactic hypersensitivity. Am J Ophthalmol. 1958 Nov;46(5 Pt 2):282–287. doi: 10.1016/0002-9394(58)90809-2. [DOI] [PubMed] [Google Scholar]
- KECKAROVSKI A. A contribution to the experimental study of the allergic processes of the anterior segment of the eye. Br J Ophthalmol. 1958 May;42(5):288–295. doi: 10.1136/bjo.42.5.288. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MORAWIECKI J. Präzipitationserscheinungen in der lebenden Hornhaut bei Antigen-Antikörperreaktionen. Ophthalmologica. 1956 Oct;132(4):236–243. doi: 10.1159/000302931. [DOI] [PubMed] [Google Scholar]
- PARKS J. J., LEIBOWITZ H. M., MAUMENEE A. E. The effect of route of inoculation upon development of antibody in rabbits. J Immunol. 1961 Aug;87:199–204. [PubMed] [Google Scholar]
- SALVIN S. B. Occurrence of delayed hypersensitivity during the development of Arthus type hypersensitivity. J Exp Med. 1958 Jan 1;107(1):109–124. doi: 10.1084/jem.107.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALVIN S. B., SMITH R. F. The specificity of allergic reactions. I. Delayed versus Arthus hypersensitivity. J Exp Med. 1960 Apr 1;111:465–483. doi: 10.1084/jem.111.4.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHWAB F. Untersuchungen über den Antikörpergehalt (Präcipitine) der Hornhaut nach gleichzeitiger Einverleibung verschiedener Antigene; ein Beitrag zur Frage der lokalen Antikörperbildung in der Hornhaut. Albrecht Von Graefes Arch Ophthalmol. 1959;160(6):592–627. [PubMed] [Google Scholar]
- WITMER R. H. Antibody formation in rabbit eye studied with fluorescein-labeled antibody. AMA Arch Ophthalmol. 1955 Jun;53(6):811–816. doi: 10.1001/archopht.1955.00930010819005. [DOI] [PubMed] [Google Scholar]