Abstract
DNA and thymine degradation on cultured human, mouse, and chick cells were studied. Significant increase in DNA-degrading activity was demonstrated in human embryonic cells killed by freeze-thawing, liver cells killed with mitomycin C, mouse embryonic cells infected with encephalomyocarditis virus, and in all cells killed by the lipovirus. Twelve other viral agents, actinomycin D, and 5-fluorodeoxyuridine failed to produce a similar increase. Thymidine-2-C14-labeled cultures, either live, killed, or infected by 19 different physical-chemical and biological agents, did not release detectable quantity of C14C2. Following infection with the lipovirus 20 to 60 per cent of the total radioactivity of thymidine-2-C14-labeled cultures was liberated as C14O2. It was postulated that the lipovirus introduced into the host cells the missing genetic information necessary for the synthesis of one or more enzymes responsible for the reductive catabolism of thymine.
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Selected References
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- CAMPBELL L. L., Jr Reductive degradation of pyrimidines. II. Mechanism of uracil degradation by Clostridium uracilicum. J Bacteriol. 1957 Feb;73(2):225–229. doi: 10.1128/jb.73.2.225-229.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CANELLAKIS E. S. Pyrimidine metabolism. I. Enzymatic pathways of uracil and thymine degradation. J Biol Chem. 1956 Jul;221(1):315–322. [PubMed] [Google Scholar]
- CHANG R. S., GEYER R. P., ANDRUS S. B. A lipogenic toxin released through the interaction of a new cytopathic agent (lipovirus) and cultured human cells. J Exp Med. 1962 May 1;115:959–966. doi: 10.1084/jem.115.5.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHANG R. S., HUMES M. The biological, immunological, and physicochemical characterization of a transmissible agent capable of inducing DNA and thymine degradation in cultured human cells. J Exp Med. 1962 May 1;115:937–958. doi: 10.1084/jem.115.5.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHANG R. S., LIEPENS H. Appearance of marked DNA-de-grading and thymine catabolic activities in a human cell infected with a transmissible agent. Proc Soc Exp Biol Med. 1961 May;107:138–141. doi: 10.3181/00379727-107-26559. [DOI] [PubMed] [Google Scholar]
- COHEN S. S. Virus-induced acquisition of metabolic function. Fed Proc. 1961 Jul;20:641–649. [PubMed] [Google Scholar]
- FOGH J., LUND R. O. Continuous cultivation of epithelial cell strain (FL) from human amniotic membrane. Proc Soc Exp Biol Med. 1957 Mar;94(3):532–537. doi: 10.3181/00379727-94-23003. [DOI] [PubMed] [Google Scholar]
- GEYER R. P., CHANG R. S. Bicarbonate as an essential for human cells in vitro. Arch Biochem Biophys. 1958 Feb;73(2):500–506. doi: 10.1016/0003-9861(58)90293-5. [DOI] [PubMed] [Google Scholar]
- HALL B. D., SPIEGELMAN S. Sequence complementarity of T2-DNA and T2-specific RNA. Proc Natl Acad Sci U S A. 1961 Feb 15;47:137–163. doi: 10.1073/pnas.47.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]