Abstract
Further data have been presented showing that the specificity of the delayed hypersensitivity reaction in the guinea pig to hapten-protein conjugates involves to a considerable degree a contribution by the protein carrier. The carrier contribution is such that sensitization to guinea pig albumin-m-azobenzenesulfonate, for example, does not result in cross-reaction with conjugates of the same hapten with unrelated proteins such as ovalbumin or human gamma globulin, nor were cross-reactions observed between conjugates prepared with the same hapten, coupled to the same protein, but by two different chemical routes, such that the point of attachment of the hapten to the protein differed. It thus appears that in this system both hapten and carrier protein are necessary, but that neither alone is in general sufficient to stimulate the delayed sensitive cell. Desensitization experiments with cross-reacting hapten-protein conjugates have suggested the presence of a multiplicity of antigenic determinants participating in the elicitation of the delayed lesion, and of a concomitant development of a heterogeneity of specificities in the population of delayed sensitive cells in the sensitized animal. The data are discussed in terms of the apparent requirement of the delayed sensitivity mechanism for a larger functional antigenic determinant than that required for interaction with circulating antibodies. Some possible explanations for this difference, and some of its consequences, are discussed.
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Selected References
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- BENACERRAF B., GELL P. G. Studies on hypersensitivity. III. The relation between delayed reactivity to the picryl group of conjugates and contact sensitivity. Immunology. 1959 Jul;2:219–229. [PMC free article] [PubMed] [Google Scholar]
- BENACERRAF B., LEVINE B. B. Immunological specificity of delayed and immediate hypersensitivity reactions. J Exp Med. 1962 May 1;115:1023–1036. doi: 10.1084/jem.115.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FRANCIS G. E., MULLIGAN W., WORMALL A. The use of radioactive isotopes in immunological investigations. 9. The reactions of antisera to antigens containing multiple determinant groups. Biochem J. 1955 Jul;60(3):370–379. doi: 10.1042/bj0600370. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GELL P. G., BENACERRAF B. Studies on hypersensitivity. IV. The relationship between contact and delayed sensitivity: a study of the specificity of cellular immune reactions. J Exp Med. 1961 Mar 1;113:571–585. doi: 10.1084/jem.113.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goebel W. F., Avery O. T., Babers F. H. CHEMO-IMMUNOLOGICAL STUDIES ON CONJUGATED CARBOHYDRATE-PROTEINS : IX. THE SPECIFICITY OF ANTIGENS PREPARED BY COMBINING THEp-AMINOPHENOL GLYCOSIDES OF DISACCHARIDES WITH PROTEIN. J Exp Med. 1934 Oct 31;60(5):599–617. doi: 10.1084/jem.60.5.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landsteiner K., van der Scheer J. ON CROSS REACTIONS OF IMMUNE SERA TO AZOPROTEINS : II. ANTIGENS WITH AZOCOMPONENTS CONTAINING TWO DETERMINANT GROUPS. J Exp Med. 1938 Apr 30;67(5):709–723. doi: 10.1084/jem.67.5.709. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SALVIN S. B., SMITH R. F. Delayed hypersensitivity and the anamnestic response. J Immunol. 1960 May;84:449–457. [PubMed] [Google Scholar]
- UHR J. W., PAPPENHEIMER A. M., Jr Delayed hypersensitivity. III. Specific desensitization of guinea pigs sensitized to protein antigens. J Exp Med. 1958 Dec 1;108(6):891–904. doi: 10.1084/jem.108.6.891. [DOI] [PMC free article] [PubMed] [Google Scholar]