Abstract
Intracellular accumulation of nitrate, indicative of the operation of an active nitrate transport system, has been measured in intact cells of the cyanobacterium Anacystis nidulans. The ability of the cells to accumulate nitrate was effectively hindered by either ammonium addition or selective inhibition of CO2 fixation by DL-glyceraldehyde, with the effect of either compound being prevented by previously blocking ammonium assimilation. The results support the contention that nitrate utilization in cyanobacteria is regulated at the level of nitrate transport through the concerted action of ammonium assimilation and CO2 fixation.
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Selected References
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- Gibson J. Nutrient transport by anoxygenic and oxygenic photosynthetic bacteria. Annu Rev Microbiol. 1984;38:135–159. doi: 10.1146/annurev.mi.38.100184.001031. [DOI] [PubMed] [Google Scholar]
- Herrero A., Flores E., Guerrero M. G. Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp. strain 7119, and Nostoc sp. strain 6719. J Bacteriol. 1981 Jan;145(1):175–180. doi: 10.1128/jb.145.1.175-180.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lara C., Romero J. M. Distinctive Light and CO(2)-Fixation Requirements of Nitrate and Ammonium Utilization by the Cyanobacterium Anacystis nidulans. Plant Physiol. 1986 Jun;81(2):686–688. doi: 10.1104/pp.81.2.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meeks J. C., Wycoff K. L., Chapman J. S., Enderlin C. S. Regulation of expression of nitrate and dinitrogen assimilation by anabaena species. Appl Environ Microbiol. 1983 Apr;45(4):1351–1359. doi: 10.1128/aem.45.4.1351-1359.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paschinger H. DCCD induced sodium uptake by Anacystis nidulans. Arch Microbiol. 1977 Jun 20;113(3):285–291. doi: 10.1007/BF00492037. [DOI] [PubMed] [Google Scholar]
- Peschek G. A., Czerny T., Schmetterer G., Nitschmann W. H. Transmembrane Proton Electrochemical Gradients in Dark Aerobic and Anaerobic Cells of the Cyanobacterium (Blue-Green Alga) Anacystis nidulans: Evidence for Respiratory Energy Transduction in the Plasma Membrane. Plant Physiol. 1985 Sep;79(1):278–284. doi: 10.1104/pp.79.1.278. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Romero J. M., Lara C., Guerrero M. G. Dependence of nitrate utilization upon active CO2 fixation in Anacystis nidulans: a regulatory aspect of the interaction between photosynthetic carbon and nitrogen metabolism. Arch Biochem Biophys. 1985 Mar;237(2):396–401. doi: 10.1016/0003-9861(85)90291-7. [DOI] [PubMed] [Google Scholar]
- Thayer J. R., Huffaker R. C. Determination of nitrate and nitrite by high-pressure liquid chromatography: comparison with other methods for nitrate determination. Anal Biochem. 1980 Feb;102(1):110–119. doi: 10.1016/0003-2697(80)90325-5. [DOI] [PubMed] [Google Scholar]