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. 1962 Oct 31;116(5):759–772. doi: 10.1084/jem.116.5.759

THE PRODUCTION OF RUNT DISEASE IN RATS THYMECTOMIZED AT BIRTH

Alan C Aisenberg 1, Barbara Wilkes 1, Byron H Waksman 1
PMCID: PMC2137556  PMID: 14011435

Abstract

Thymectomy of Sprague-Dawley rats on the 3rd day of life failed to influence the time of onset, incidence, clinical, or histologic picture of runt disease produced by the intraperitoneal injection of adult Long-Evans spleen cells. The fact that severe immunologic impairment of the host by thymectomy does not modify runt disease was felt to be consistent with the current view that the if direction of the immunologic event in this syndrome is graft versus host. Following the injection of 800 to 1000 million Long-Evans spleen cells into adult Sprague-Dawley rats, a severe illness comprised of dermatitis, gastrointestinal bleeding, arthritis, weight loss, and death ensued in 37 per cent of adults thymectomized neonatally and 13 per cent of normal controls. Histologic lesions were observed in 69 per cent of adequately thymectomized animals and 17 per cent of normal controls, and involved lymph nodes, spleen, liver, lungs, kidneys, joints, heart, and skin. The time of onset and the histologic and clinical pictures are consistent with the adult disease being a typical graft versus host reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON N. F., DELORME E. J., WOODRUFF M. F. Induction of runt disease in rats by injection of thoracic duct lymphocytes at birth. Transplant Bull. 1960 Jan;7:93–97. doi: 10.1097/00006534-196001000-00033. [DOI] [PubMed] [Google Scholar]
  2. BARNES D. W., FORD C. E., ILBERY P. L., KOLLER P. C., LOUTIT J. F. Tissue transplantation in the radiation chimera. J Cell Physiol Suppl. 1957 Dec;50(Suppl 1):123–138. doi: 10.1002/jcp.1030500409. [DOI] [PubMed] [Google Scholar]
  3. BILLINGHAM R. E., DEFENDIV, SILVERS W. K., STEINMULLER D. Quantitative studies on the induction of tolerance of skin homografts and on runt disease in neonatal rats. J Natl Cancer Inst. 1962 Feb;28:365–435. [PubMed] [Google Scholar]
  4. BILLINGHAM R. E., SILVERS W. K. The induction of tolerance of skin homografts in rats with pooled cells from multiple donors. J Immunol. 1959 Dec;83:667–679. [PubMed] [Google Scholar]
  5. BRENT L., BROWN J., MEDAWAR P. B. Skin transplantation immunity in relation to hypersensitivity. Lancet. 1958 Sep 13;2(7046):561–564. doi: 10.1016/s0140-6736(58)90202-2. [DOI] [PubMed] [Google Scholar]
  6. CASTERMANS A. Reevaluation of a pretreatment given to adult animals to modify their responsiveness to skin homografts. Transplant Bull. 1958 Oct;5(4):381–387. doi: 10.1097/00006534-195810000-00035. [DOI] [PubMed] [Google Scholar]
  7. CONGDON C. C., URSO I. S. Homologous bone marrow in the treatment of radiation injury in mice. Am J Pathol. 1957 Jul-Aug;33(4):749–767. [PMC free article] [PubMed] [Google Scholar]
  8. DAVIES A. J., DOAK S. M. Fate of homologous adult spleen cells injected into new-born mice. Nature. 1960 Aug 13;187:610–611. doi: 10.1038/187610b0. [DOI] [PubMed] [Google Scholar]
  9. DENKO J. D., SIMMONS E. L., WISSLER R. W. The histopathology of delayed death in irradiated mice treated with homologous cells. Radiat Res. 1959 Oct;11:557–571. [PubMed] [Google Scholar]
  10. EICHWALD E. J., LUSTGRAAF E. C., STRAINER M. Genetic factors in parabiosis. J Natl Cancer Inst. 1959 Dec;23:1193–1213. [PubMed] [Google Scholar]
  11. HOWARD J. G., MICHIE D., SIMONSEN M. Splenomegaly as a host response in graft-versus-host disease. Br J Exp Pathol. 1961 Oct;42:478–485. [PMC free article] [PubMed] [Google Scholar]
  12. JANKOVIC B. D., WAKSMAN B. H., ARNASON B. G. Role of the thymus in immune ractions in rats. I. The immunologic response to bovine serum albumin (antibody formation, Arthus reactivity, and delayed hypersensitivity) in rats thymectomized or splenectomized at various times after birth. J Exp Med. 1962 Aug 1;116:159–176. doi: 10.1084/jem.116.2.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KAPLAN H. S., NAGAREDA C. S., ROSSTON B. B., BROWN M. B. Comparative activity of isologous versus homologous mouse bone marrow on lymphoid tumor production following irradiation. Proc Soc Exp Biol Med. 1958 Jun;98(2):384–387. doi: 10.3181/00379727-98-24051. [DOI] [PubMed] [Google Scholar]
  14. KOLLER P. C., DAVIES A. J., DOAK S. M. Radiation chimeras. Adv Cancer Res. 1961;6:181–289. doi: 10.1016/s0065-230x(08)60622-3. [DOI] [PubMed] [Google Scholar]
  15. NAJARIAN J. S., FELDMAN J. D. Induction of runt disease in adult mice by pre-sensitized homologous lymphoid cells. Proc Soc Exp Biol Med. 1962 May;110:16–21. doi: 10.3181/00379727-110-27407. [DOI] [PubMed] [Google Scholar]
  16. NISBET N. W., HESLOP B. F. Runt disease. Br Med J. 1962 Jan 20;1(5272):129–contd. doi: 10.1136/bmj.1.5272.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PORTER K. A. Immune hemolysis: a feature of secondary disease and runt disease in the rabbit. Ann N Y Acad Sci. 1960 May 31;87:391–402. doi: 10.1111/j.1749-6632.1960.tb23208.x. [DOI] [PubMed] [Google Scholar]
  18. SCHWARTZ E. E., UPTON A. C., CONGDON C. C. A fatal reaction caused by implantation of adult parental spleen tissue in irradiated F1 mice. Proc Soc Exp Biol Med. 1957 Dec;96(3):797–800. doi: 10.3181/00379727-96-23612. [DOI] [PubMed] [Google Scholar]
  19. SIMONSEN M. Graft versus host reactions. Their natural history, and applicability as tools of research. Prog Allergy. 1962;6:349–467. [PubMed] [Google Scholar]
  20. SIMONSEN M. The impact on the developing embryo and newborn animal of adult homologous cells. Acta Pathol Microbiol Scand. 1957;40(6):480–500. [PubMed] [Google Scholar]
  21. TRENTIN J. J. The immunological basis for induced tolerance to skin homografts in irradiated mice receiving bone marrow transfusions. Transplant Bull. 1957 Apr;4(2):74–76. [PubMed] [Google Scholar]
  22. TRENTIN J. J. Tolerance and homologous disease in irradiated mice protected with homologous bone marrow. Ann N Y Acad Sci. 1958 Oct 7;73(3):799–810. doi: 10.1111/j.1749-6632.1959.tb40859.x. [DOI] [PubMed] [Google Scholar]
  23. UPHOFF D. E. Genetic factors influencing irradiation protection by bone marrow. I. The F1 hybrid effect. J Natl Cancer Inst. 1957 Jul;19(1):123–130. [PubMed] [Google Scholar]
  24. WAKSMAN B. H., ARNASON B. G., JANKOVIC B. D. Role of the thymus in immune reactions in rats. III. Changes in the lymphoid organs of thymectomized rats. J Exp Med. 1962 Aug 1;116:187–206. doi: 10.1084/jem.116.2.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WOODRUFF M. F., SPARROW M. Further observation on the induction of tolerance of skin homografts in rats. Transplant Bull. 1957 Oct;4(4):157–159. [PubMed] [Google Scholar]
  26. van BEKKUM D., VOS O., WEYZEN W. W. The pathogenesis of the secondary disease after foreign bone marrow transplantation in X-irradiated mice. J Natl Cancer Inst. 1959 Jul;23:75–89. [PubMed] [Google Scholar]

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